Mass-marking of brown trout (Salmo trutta L.) larvae by alizarin: method and evaluation of stocking

Baer, Jan; Rösch, Roland

doi:10.1111/j.1439-0426.2007.01038.x

Cited by 42 publications

(60 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite variations in the quality and lasting of the marks, fluorescent signals were detected in both treatments and all the structures analyzed. These findings are in agreement with several studies that showed ARS effectiveness for immersion marking, as well as its potential in biological research and evaluation of stock enhancement programs (Bashey, 2004;Baer & Rösch, 2008;Crook et al, 2007;Liu et al, 2009).…”

Section: Discussionsupporting

confidence: 82%

“…The most commonly used were oxytetracycline (OTC), hydrochloride, calcein, alizarin complexone (AC) and alizarin red S (ARS). However, calcein, ARS and AC performed better than other chemicals because: marks can be detected and read clearly, the effect over survival rates was proven to be less negative, and all can be used in salt water (Tsukamoto et al, 1989;Van der Walt & Faragher, 2003;Taylor et al, 2005;Baer & Rösch, 2008;Liu et al, 2009). Even though previously performed experiments using calcein have obtained clear and long-lasting marks, this chemical compound is expensive and was found to be toxic when fishes were exposed to it for long periods of time (Brooks et al, 1994;Bumguardner & King, 1996;Frenkel et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

et al. 2013

View full text Add to dashboard Cite

Juveniles of pejerrey, Odontesthes bonariensis, were exposed to 0.1% Alizarin Red S (ARS) alone or with a previous immersion in 2.2% saline solution (Osmotic Induction, OI) to enhance the ARS marking method. Fish were marked in the field and immediately released in 1 m 3 cages in "La Salada de Monasterio" lagoon, Chascomús, Buenos Aires, Argentina. After 73 days, clear marks were observed in the otoliths, caudal fin rays and scales with both treatments, being the intensity of the signal in the scales of OI+ARS treated fish higher. On the other hand, no marks were observed in the control group on the same structures. Approximately one year post-treatment (385 days), only marks in caudal fin rays were found clearly in OI+ARS treated fish. After this period, no significant differences in total length or weight between marked or control fish were observed and the mortality ranged between 30-40 % in all cages. These results provide strong evidence for the potential applicability of this cost-effective marking technique in differentiation of wild and hatchery-produced pejerrey. The success in the caudal fin rays marking is also important because it is easy to do and does not require the sacrifice of fish.Juvenis de pejerrey, Odontesthes bonariensis, foram expostos a Vermelho de Alizarina S (ARS) 0,1% de duas formas, sozinho ou com uma imersão anterior em 2,2% de solução salina (Indução osmótica, IO) para melhorar o método de marcação ARS. Os procedimentos foram realizados no campo e os peixes foram liberados em gaiolas (tanques-rede) de 1 m 3 na lagoa "La Salada de Monasterio", Chascomús, Buenos Aires, Argentina. Após 73 dias, marcas claras foram observadas nos otólitos, raios da nadadeira caudal e escamas em ambos os tratamentos, sendo que a intensidade do sinal nas escalas de IO + ARS de peixe tratado foi superior. Por outro lado, não foram observados marcas no grupo controle sobre as mesmas estruturas. Aproximadamente um ano pós-tratamento (385 dias), apenas marcas nos raios da nadadeira caudal foram encontrados claramente nos peixes tratados com IO+ARS. Entre os peixes observados, após este período, não houve diferenças significativas no comprimento total ou peso entre o grupo controle e marcados, ademais, a mortalidade variou entre 30-40% em todas as gaiolas. Estes resultados fornecem fortes evidências e um grande potencial para aplicação desta técnica rentável de marcação que diferencia o pejerrey selvagem e o produzido em cativeiro. O sucesso na marcação dos raios da nadadeira caudal também é de grande importância, pois sua verificação é fácil e não requer o sacrifício de peixes.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The present study approves the method of mu ltip le marking of sea trout larvae with a 3h immersion in a 200 mg A RS L -1 solution without requiring prior osmotic induction. This corresponds with the results presented in previous studies where 100% effect ive ARS concentrations for batch marking of Salmo trutta L. larvae ranged between 100-150 mg ARS L -1 , which also kept fish mo rtality below 5% (Baer & Rösch, 2008;Caudron & Champigneulle, 2006, 2009Niva et al, 2005;Unfer & Pinter, 2013). At higher alizarin concentrations, up to 300 mg ARS L -1 , high fish mortality (about 95%) was observed immed iately after a 3h immersion (Baer & Rösch, 2008).…”

Section: Discussionsupporting

confidence: 70%

“…This corresponds with the results presented in previous studies where 100% effect ive ARS concentrations for batch marking of Salmo trutta L. larvae ranged between 100-150 mg ARS L -1 , which also kept fish mo rtality below 5% (Baer & Rösch, 2008;Caudron & Champigneulle, 2006, 2009Niva et al, 2005;Unfer & Pinter, 2013). At higher alizarin concentrations, up to 300 mg ARS L -1 , high fish mortality (about 95%) was observed immed iately after a 3h immersion (Baer & Rösch, 2008). Different results were reported by Unfer & Pinter (2013), who recorded a post-marking mortality rate of brown trout larvae of 0.8 Ind./day during 38 days, after immersion in 300 mg A RS L -1 for 3h.…”

Section: Discussionsupporting

confidence: 67%

“…In recent years, alizarin red S (ARS) has been one of the most commonly used marking substances which has been applied to Salmo trutta L., immersed at different life stages, including, chronologically, embryos (Unfer & Pinter, 2013), alevins (Baer & Rösch, 2008;Caudron & Champigneulle, 2006, 2009Niva et al, 2005;Unfer & Pinter, 2013) and fry (Caudron & Champigneulle, 2009;Gerdeau x et al 2006;Unfer & Pinter, 2013). Only two papers make references to mult iple A RS marking of Salmo trutta L. otoliths (Caudron & Champigneulle, 2006, 2009.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Lejk¹,

Martyniak²

2018

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

This study presents a protocol of immersing sea trout Salmo trutta m. trutta L. larvae within an alizarin red S (ARS) solution to provide highly visible single and double fluorescent marks in their otoliths. The 14 day interval and temperature regime between the first and second batch marking sessions have been found to develop the proper distance between the ARS marks in the otoliths. The mean values of increment widths between the first and second ARS marks in the otoliths of sea trout marked twice within 14 day period ranged between 21.11-34.98 μm, depending on the year of the experiment. The distance between ARS marks corresponded to temperature during the period between first and second fish immersions which was 4.4ºC, 7.5ºC and 6.7ºC, for year 2014, 2015, and 2016 respectively. As a result, this method can be used as a routine procedure that provides clear and well-visible single and double alizarin marks in the otoliths and helps to distinguish fish originating from different stocking groups of wild-born individuals.

show abstract

River damming drives population fragmentation and habitat loss of the threatened Danube streber (Zingel streber): Implications for conservation

Brinker

Chucholl²,

Behrmann‐Godel

et al. 2018

Aquatic Conservation

View full text Add to dashboard Cite

1. The Danube streber, Zingel streber, is a threatened and data-deficient percid fish endemic to the Danube catchment. The study provides the first data on distribution, life history, and genetic structure of the species at the upstream limit of its historic distribution (south-western Germany). A 3-year surveyeffort with 143 fishing events identified several small, fragmentary populations covering only 7% of the historical range of the species. Census population sizes (N c ) of these subpopulations were estimated from mark-recapture data at <200 individuals. Effective population sizes (N e ), calculated from genetic data (microsatellite genotyping), were much smaller still, at <15 individuals, resulting in an N c /N e ratio of <0.25, strongly indicating that populations are seriously affected by genetic drift and inbreeding, and are thus facing a severe extinction risk. 3. Life-history parameters recorded during the study indicate a rapid life cycle, with both sexes probably attaining sexual maturity at the age of 1 year or older. Spawning commenced at the beginning of April and fecundity was low (~300-400 eggs per female). 4. Genetic analysis and mark-recapture data indicate that subpopulations of the streber live in effective isolation, separated by impassable weirs that significantly reduce genetic connectivity between subpopulations. 5. The species is rheophilic, and limited to sites with flow velocities of~0.7 m s -1 . Hydropower infrastructure may thus also have diminished the availability of suitable habitat by reducing flow rates. 6. Only 32% of the historical range of the Danube streber is now estimated to be morphologically suitable for the species. Furthermore, relevant parts of this range are located upstream of dams and are therefore not accessible for natural recolonization. 7. The availability and accessibility of suitable habitats seem to be factors limiting the size of the remaining subpopulations. 8. Conservation actions should address the restoration of degraded river habitats and increase the connectivity between isolated subpopulations of the Danube streber.

show abstract

Mass-marking of brown trout (Salmo trutta L.) larvae by alizarin: method and evaluation of stocking

Abstract: SummaryThis study describes otolith marking of brown trout (Salmo trutta L.) larvae by immersion in different solutions of alizarin red S (ARS). The best results were obtained after marking with ARS at a concentration of 150 mg L )1

Cited by 42 publications

References 35 publications

Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

Untitled

River damming drives population fragmentation and habitat loss of the threatened Danube streber (Zingel streber): Implications for conservation

Contact Info

Product

Resources

About