Mass Mortality of Captive Southern Bluefin Tuna (Thunnus maccoyii) in April/May 1996 in Boston Bay, South Australia: A Complex Diagnostic Problem.

Munday, B. L.; Hallegraeff, Gustaaf M.

doi:10.3147/jsfp.33.343

Cited by 34 publications

(23 citation statements)

References 17 publications

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“…A likely scenario for mortality caused by Chattonella spp. is that fish die because of the direct toxic effect of oxygen radicals on the gills in combination with the cardiotoxic effects of brevetoxins (Ishimatsu et al, 1990;Hallegraeff et al, 1998;Munday and Hallegraeff, 1998). …”

Section: Chattonella Sppmentioning

confidence: 96%

The Effects of Harmful Algal Blooms on Aquatic Organisms

Landsberg

2002

Reviews in Fisheries Science

1,135

807

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Section: Chattonella Sppmentioning

confidence: 96%

The Effects of Harmful Algal Blooms on Aquatic Organisms

Landsberg

2002

Reviews in Fisheries Science

1,135

807

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“…During HAB events, fishery and aquaculture closures cause considerable loss to local economies. For example, a toxic dinoflagellate bloom of Alexandrium tamarense in Tasmania in 2012 cost the local economy $23 million AUD through the closure of shellfish farms and seafood product recall (Campbell, Hudson, Mcleod, Nicholls, & Pointon, ), and a Chattonella raphidophyte bloom in South Australia in 1996 killed $45 million AUD of aquaculture tuna fish (Munday & Hallegraeff, ).…”

Section: Introductionmentioning

confidence: 99%

Retrospective eDNA assessment of potentially harmful algae in historical ship ballast tank and marine port sediments

et al. 2019

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Microalgal bloom events can cause major ecosystem disturbances, devastate local marine economies, and endanger public health. Therefore, detecting and monitoring harmful microalgal taxa is essential to ensure effective risk management in waterways used for fisheries, aquaculture, recreational activity, and shipping. To fully understand the current status and future direction of algal bloom distributions, we need to know how populations and ecosystems have changed over time. This baseline knowledge is critical for predicting ecosystem responses to future anthropogenic change and will assist in the future management of coastal ecosystems. We explore a NGS metabarcoding approach to rapidly identify potentially harmful microalgal taxa in 63 historic and modern Australian marine port and ballast tank sediment samples. The results provide a record of past microalgal distribution and important baseline data that can be used to assess the efficacy of shipping guidelines, nutrient pollution mitigation, and predict the impact of climate change. Critically, eDNA surveys of archived sediments were able to detect harmful algal taxa that do not produce microscopic fossils, such as Chattonella, Heterosigma, Karlodinium, and Noctiluca. Our data suggest a potential increase in Australian harmful microalgal taxa over the past 30 years, and confirm ship ballast tanks as key dispersal vectors. These molecular mapping tools will assist in the creation of policies aimed at reducing the global increase and spread of harmful algal taxa and help prevent economic and public‐health problems caused by harmful algal blooms.

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“…Harmful algae belonging to the class Raphidophyceae are widespread and have been linked to mass mortalities of fish in many areas of the world (Odebrecht and Abreu 1995;Munday and Hallegraeff 1998), including Delaware's Inland Bays (DIB) (Bourdelais et al 2002). Using species-specific primers and probes, raphidophyte species from the DIB have been accurately enumerated by QPCR in simplex reactions Handy et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous enumeration of multiple raphidophyte species by quantitative real-time PCR: capabilities and limitations

Handy

Hutchins

Cary

et al. 2006

Limnol. Oceanogr. Methods

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Quantitative real-time PCR (QPCR) is a powerful method for species-specific detection and enumeration of harmful algal bloom (HAB) algae, but can be quite time-consuming and expensive for multiple species detection when only a single species is analyzed in each reaction. Rapid enumeration methods would be valuable for the investigation of multiple HAB events such as the mixed blooms of harmful raphidophyte species (Chattonella cf verruculosa, C. subsalsa, and Heterosigma akashiwo) that co-occur in the Delaware Inland Bays (DIB), USA. This technology uses multiple species-specific probes that fluoresce at different wavelengths and allows for the simultaneous detection and enumeration of more than one species, limited only by the detection capabilities of the instrument. The approach can cut analysis time and cost at least in half, depending on the number of species that can be accurately detected and enumerated in the mixture. Here, we compared multiprobing-or using a single primer set with species-specific probes-to multiplexing, in which species-specific primers and probes are used, targeting the 18S rDNA of 3 raphidophyte species and an internal standard. Although both methods were effective for multispecies detection compared to simplex results, multiplexing was more accurate than multiprobing for this particular group of species. We investigated the accuracy and sensitivity of multiplex/multiprobe QPCR using plasmids, genomic DNA from cultures, or DNA extracted from environmental samples as template. Smaller amplicon size and sequence heterogeneity between amplicons increased the accuracy of the results. We found that environmental samples of raphidophytes can be successfully multiplexed or multiprobed with only minimal losses in sensitivity. In addition, we explored probe reporter dyes and quenchers for their compatibility, robustness, and reproducibility in multitemplate detection, and we compared and evaluated the capabilities and limitations of this method for detecting and enumerating multiple phytoplankton species simultaneously.

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Mass Mortality of Captive Southern Bluefin Tuna (Thunnus maccoyii) in April/May 1996 in Boston Bay, South Australia: A Complex Diagnostic Problem.

Cited by 34 publications

References 17 publications

The Effects of Harmful Algal Blooms on Aquatic Organisms

The Effects of Harmful Algal Blooms on Aquatic Organisms

Retrospective eDNA assessment of potentially harmful algae in historical ship ballast tank and marine port sediments

Simultaneous enumeration of multiple raphidophyte species by quantitative real-time PCR: capabilities and limitations

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