Mass production of axenic spores of the endomycorrhizal fungus Gigaspora margarita

Mertz, Stuart M.; Heithaus, J. J.; Bush, R.L.

doi:10.1016/s0007-1536(79)80024-8

Cited by 50 publications

(23 citation statements)

References 7 publications

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“…All depend on differential sedimentation to separate spores and organic debris from the heavier particles in a soil suspension (Gerdemann, 1955a). Variations of this are a floatation-adhesion technique (Sutton and Barron, 1972), a countercurrent floatation-bubbling system (Furlan and Fortin, 1975), and a separation by centrifugation on discontinuous sucrose gradients (Mertz et al, 1979) that has been proposed for large-scale spore separation. Unless exposure to high osmotic pressures is very short, spores can be damaged.…”

Section: Va Endophytesmentioning

confidence: 99%

“…They may then germinate again (Mosse, 1959) up to ten or more times (Koske, 1981a) if the original germ tube is removed. The spores are relatively resistant to desiccation and survive well at low temperatures (Tommerup and Kidby, 1979;Mertz et al, 1979). The mycelium also has an unusual ability to repair damaged sections (Gerdemann, 1955b) and to move cytoplasmic contents, forming septa to cut off empty, nonfunctional portions.…”

Section: Survival Of Fungi After Disappearance Of the Hostmentioning

confidence: 99%

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Ecology of Mycorrhizae and Mycorrhizal Fungi

Mosse

Stribley

LeTacon³

1981

Advances in Microbial Ecology

225

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Section: Va Endophytesmentioning

confidence: 99%

Section: Survival Of Fungi After Disappearance Of the Hostmentioning

confidence: 99%

Ecology of Mycorrhizae and Mycorrhizal Fungi

Mosse

Stribley

LeTacon³

1981

Advances in Microbial Ecology

225

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“…Walker and Koske and Glomus heterosporum Smith & Schenck) were isolated from the rhizosphere soils of natural ecosystem of Purna river saline tract of Amravati District. The isolation of AM spores was carried out by the wet-siewing and decanting technique (Gerdemann and Nicolson, 1963) and the isolated spores were identified on the basis of their morphological characters given in the identification manual of AM fungi (Schenk and Perez, 1990) and key of Mortan and Benny (1990).AMF spores of the most dominant species were purified and multiplied by following funnel technique (Mertz et al,1979). The pure culture of isolated AM fungi was used for inoculation for Glycine max.…”

Section: Methodsmentioning

confidence: 99%

Efficiency of Indigenous AMF Species on Growth of Soybean (Glycine max L.) under Varying Levels of Phosphorous in Saline Condition

2016

IJSR

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Abstract:The pot experiments were undertaken to study the relative efficiency of dominant AM Fungi (Glomus fasciculatum and Glomus heterosporum) recovered from natural undisturbed saline belt of Purna river sub basin. The pure inoculum of these species were treated individually and as a mixed inocula in combination with phosphoric fertilizers supplied as 25, 50, 75 and 100% (mg/kg P 2 O 5 /ha). The influence of AMF inoculation in different levels of 'P' fertilizer on% colonization, MIE % and P-uptake were calculated. Plevel of 75% was found to be better in compare to 100% and can be recommended as phosphoric dose for soybean plants.

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“…The disinfection process was modified from Mosse [36], Mertz et al [37], Daniels and Menge [38], and Bécard and Fortin [31]. Isolated propagules were first rinsed twice with sterilized distilled water after which they were disinfected.…”

Section: Am Fungal Propagule Disinfection Processmentioning

confidence: 99%

Maximizing the Shelf Life of Monoxenically Produced Carrier-Based Am Fungal Bio-Inocula for Maintaining Its Long-Term Viability

Rodrigues

Rodrigues²

2018

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Arbuscular mycorrhizal (AM) fungi are often applied as bio-inoculants due to their plant growth promoting benefits. The objectives of the present work were to maximize the shelf life of monoxenically produced carrier-based AM fungal bio-inocula of Rhizoglomus intraradices and Funneliformis mosseae. Shelf life of in vitro produced inoculum was studied by assessing the infectivity potential of in vitro produced inocula in an optimum carrier formulation (vermiculite: cow dung powder: wood powder: wood ash in the ratio of 20:8:2:1) during storage at three different temperatures viz., 4 °C, 25 °C and room temperature (RT). The re-germination potential of in vitro produced spores from carrier-based inocula to in vitro conditions was also examined. The in vitro produced inocula stored at 25 °C remained viable up to 6 mo in the organic carrier formulation. 100 % germination was recorded when the spores of both AM species were cultured back to in vitro conditions indicating high viability, and efficiency of the carrier formulation in maintaining vigour of in vitro produced propagules.

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Mass production of axenic spores of the endomycorrhizal fungus Gigaspora margarita

Cited by 50 publications

References 7 publications

Ecology of Mycorrhizae and Mycorrhizal Fungi

Ecology of Mycorrhizae and Mycorrhizal Fungi

Efficiency of Indigenous AMF Species on Growth of Soybean (Glycine max L.) under Varying Levels of Phosphorous in Saline Condition

Maximizing the Shelf Life of Monoxenically Produced Carrier-Based Am Fungal Bio-Inocula for Maintaining Its Long-Term Viability

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