Mass-Production of Biocatalyst-Entrapping Alginate Gel Particles by a Forced Oscillation Method

Gotoh, Takehiko; Unno, Hajime; Shiragami, Naohiro; Honda, Haruo; Naoki, Y.

doi:10.1080/00986449308936125

Cited by 6 publications

(2 citation statements)

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“…He succeeded in producing monodisperse beads of 20-80 µm, but cell concentrations were low due to their goal of having one single cell per bead. Gotoh (1993) immobilized yeast with low concentrations, and Hulst et al (1985) investigated bead characteristics with yeast and plant cells working with concentration smaller than 2.3 g dry wt/L, which corresponds to a volume fraction of approximately 0.7%. In this concentration range and with nozzle diameters of 0.8 mm no influence of the suspended cells on the size distribution of the beads could be detected.…”

Section: Jet Breakup Phenomena and Rheological Description Of Suspensmentioning

confidence: 99%

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Immobilization of Highly Concentrated Cell Suspensions Using the Laminar Jet Breakup Technique

Brandenberger

Widmer

1999

Biotechnol. Prog.

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The controlled breakup of a disturbed jet is a well-known technique for monodisperse particle production and has been well-investigated for Newtonian and viscoelastic fluids. For the immobilization of cells in beads of a hydrogel it has been observed that there is a maximum cell concentration beyond which a monodisperse bead distribution cannot be reached. Higher concentrations lead to irregular jet breakup and thus to droplet coalescence forming beads with double or triple volume. This maximum cell concentration has been investigated for different operating conditions using latex beads of different diameters as a model substance for cells and baker's yeast as a comparison. The maximum cell concentration where a monodisperse size distribution could still be achieved was increased using an electrostatic droplet dispersion system.

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Section: Jet Breakup Phenomena and Rheological Description Of Suspensmentioning

confidence: 99%

“…For higher productivity and smaller monodisperse bead diameters the laminar jet breakup technique is used (Hulst et al, 1985;Gotoh et al, 1993;Seifert and Phillips, 1997). The jet breakup phenomenon is well-described (Eggers, 1997), but little is known about the dynamics of suspensions.…”

Section: Introductionmentioning

confidence: 99%