Mass Production of Entomopathogenic Fungi (Metarhizium anisopliae) using Different Grains as a Substrate

Agale, S.V.; Gopalakrishnan, Subramaniam; Ambhure, K.G.; Chandravanshi, Hemkant; Gupta, Rajeev; Wani, S P

doi:10.20546/ijcmas.2018.701.268

Cited by 9 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The research was designed in a randomized block design with 3 treatments, namely (1) botanical pesticides (a mixture of neem oil: citronella oil: clove oil) with the ratio of 1: 1: 1; (2) biological pesticides from B. bassiana and ( 3) control (water) and nine replications. B. bassiana, first being propagated or grown on Potato Dextrose Agar (PDA) medium, then on corn medium for about two weeks [16,17]. Meanwhile, the botanical pesticide formulation is carried out by mixing neem oil, citronella essential oil, clove essential oil in the ratio of 1: 1: 1 and was added with 2% emulsifier (Tween 80), in order to be a water soluble solution.…”

Section: Methodsmentioning

confidence: 99%

Effect of biopesticides against stem borer (Lophobaris piperis) and Thrips sp. on pepper (Piper nigrum)

Kardinan

Maris

2021

E3S Web Conf.

View full text Add to dashboard Cite

One of the pepper crop production obstacles is the presence of pests. Several pests that often attack pepper plants in the Sukamulya Experimental Station is pepper stem borer (Lophobaris piperis) and Thrips sp. We studied the effect of biopesticides of botanical pesticides and biological pesticides at the Sukamulya Experimental Station, Sukabumi – West Java in 2020 against the pepper stem borer L. piperis and Thrips sp. in pepper plantation. The research was designed in a randomized block with three treatments, namely (1) botanical pesticides, (2) biological pesticides and (3) control (water), and nine replications. The treatment application is carried out once a month, done immediately after observing the intensity of the pest attack. The results showed that the attack of pepper stem borer was low (below 10%), so the effect of the treatment could not be seen significantly. The Thrips attack was high enough, it was seen that there was the ability of botanical pesticides to reduce the intensity of the attack. Meanwhile, the biological pesticide had not yet shown its ability to reduce the intensity of Thrips sp.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of biopesticides against stem borer (Lophobaris piperis) and Thrips sp. on pepper (Piper nigrum)

Kardinan

Maris

2021

E3S Web Conf.

View full text Add to dashboard Cite

show abstract

“…Similarly, Agale et al [19] used ten different substrates, including chickpea, pigeon pea, black gram, maize, sorghum, soybean, rice, wheat, ground nut, and green gram, as well as two media, PDA and SDA, for the mass production of the entomopathogenic fungus M. anisopliae. The results showed that the highest conidial count (67.6×10…”

Section: Lecanicillium Lecanii Hirsutella Thompsonii Andmentioning

confidence: 99%

“…The results revealed that maximum yield 278.75×10 , 171.75×10 6 , 34.25×10 6 and 39.00×10 6 spores per ml) [15]. similarly, Agale [19], were used ten different substrates (chickpea, pigeon pea, black gram, maize, sorghum, soybean, rice, wheat, ground nut and green gram) and two media like PDA and SDA for mass production of entomopathogenic fungi M. anisopliae. The result revealed that significantly highest conidial count (67.6 × 10 3 spores/ml) was observed on green gram followed by sorghum in 10…”

Section: Lecanicillium Lecanii Hirsutella Thompsonii Andmentioning

confidence: 99%

Evaluating the Stability of Post Mushroom Substrate (PMS) and Other Agro-wastes for Mass Production of Entomopathogenic Fungi

Ranadev

Nagaraju

Kumari

et al. 2023

CJAST

View full text Add to dashboard Cite

Aim: The study was undertaken to evaluate agro-wastes (Post mushroom substrate, Sugarcane bagasse, rice husk and sorghum grains) for mass production of entomopathogenic fungi like, I. fumosoroseus:MT997932, B. bassiana: MT997933, L. lecanii: MT997935 and H. thompsonii: MT997936 by solid stat fermentation. Place and Duration of Work: The study was carried out in the Department of Agricultural Microbiology, GKVK, University of Agricultural Sciences, Bangalore during 2019-20. Methodology: The substrates were dried, chopped and sieved through 2mm sieve. 100g of all substrates were sterilized and moistened to 60% by adding sterile distilled water fallowed by addition of 5ml of spore suspension of fungal isolates. Treatments T5. T6, T7, T8 and T10 were fortified with adding 10ml of molasses. Observation like, growth and spore production were taken at 7, 14 and 21 days after inoculation. Results: The growth and spore production of entomopathogenic fungal isolates were observed to be increased accordance with the incubation period. Among four substrates maximum mycelial growth and spore production of all the isolates (×109 spores g-1) was observed in sorghum grains (T4:T8) fallowed by treatment T7 (SMS+10% molasses) on 21 DAI. Whereas, sugarcane bagasse and paddy husk substrates were not supported the satisfactory growth and spore production besides the addition of molasses (×107 spores g-1). The addition of molasses has positively influenced the growth and spore production of entomogenus isolates in all treatments. Conclusion: Based on results, it is evidenced that even though cereal grains are best option for mass production, PMS fortified with molasses will become a better substrate for mass production and reduce the load of using food grains as substrate.

show abstract

“…Considerados microorganismos versáteis e seguros ao meio ambiente, os fungos são responsáveis por aproximadamente 80 % das doenças entomopatológicas em insetos (AGALE et al, 2018;ROBBS e BITTENCOURT, 1998).…”

Section: Modo De Ação Dos Fungos Entomopatógenosunclassified

Fungos: Biodiversidade E Biotecnologia, Um Breve Panorama

Neto¹

2019

Principais Grupos E Aplicações Biotecnológicas Dos Fungos

View full text Add to dashboard Cite

Mass Production of Entomopathogenic Fungi (Metarhizium anisopliae) using Different Grains as a Substrate

Cited by 9 publications

References 5 publications

Effect of biopesticides against stem borer (Lophobaris piperis) and Thrips sp. on pepper (Piper nigrum)

Effect of biopesticides against stem borer (Lophobaris piperis) and Thrips sp. on pepper (Piper nigrum)

Evaluating the Stability of Post Mushroom Substrate (PMS) and Other Agro-wastes for Mass Production of Entomopathogenic Fungi

Fungos: Biodiversidade E Biotecnologia, Um Breve Panorama

Contact Info

Product

Resources

About