Mass Production of Entomopathogenic Nematodes- A Review

Devi, Gitanjali

doi:10.22161/ijeab/3.3.41

Cited by 11 publications

(7 citation statements)

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“…There are many lepidopteron, coleopteran and dipteran insect hosts that have been used for in vivo production of EPNs (Devi 2018). The host used in this study was T. castaneum, a member of the order coleoptera and the same family of Tenebrio molitor (Tenibrionidae), which were used by many researchers previously (Ehlers andShapiro-Ilan 2005, Devi 2018) for in vivo EPNs production.…”

Section: Discussionmentioning

confidence: 99%

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Mass production of the nematode Acrobeloides longiuterus using Tribolium castaneum and artificial solid media

2021

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Free-living nematode Acrobeloides longiuterus (Rhabditida: Cephalobidae) exhibits a potential to kill some insect pests. Mass production of this species is a requirement for use it in pest management programs. Tribolium castaneum has been used as a primary host for this nematode as an alternative for Galleria mellonella. Use of artificial media is another option for mass culturing and such recipes based on soy flour are available. Production of A. longiuterus using cost effective method and easily available insect host is important in setting up of small-scale production unit. Therefore, this study has the objectives of evaluating the production feasibility of A. longiuterus on T. castaneum larvae, pupae and adults as in vivo production method. Further, feasibility of using different solid media such as soy flour, palmyra tuber flour, corn flour, black gram flour and dhal flour with other basic ingredients as in vitro conditions system was evaluated. Results revealed that pupa of T. castaneum yielded the highest number of infective juveniles (36112 IJs/ pupa) compared to other life stages tested. In vitro production of A. longiuterus on soy flour and black gram flour media yielded 21530 and 16538 IJs/20g, respectively. Pathogenicity against T. castaneum was shown up to 93% by the infective juveniles produced from the in vitro cultures. In conclusion, T. castaneum is an alternative insect that can be used as a host to produce the A. longiuterus. In addition, soy flour and black gram flour can be used as the sources for this nematode production without losing their entomopathogenicity.

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Section: Discussionmentioning

confidence: 99%

“…Sterilized medium is inoculated with bacteria followed by the inoculation of EPNs. Infective juveniles could be collected from the second week after inoculation of EPNs and collected IJs could be stored in water (Devi 2018). House et al (1965) developed a dog food based medium for commercial scale production of EPNs.…”

Section: Introductionmentioning

confidence: 99%

Mass production of the nematode Acrobeloides longiuterus using Tribolium castaneum and artificial solid media

2021

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“…Mass rearing is carried out in large bioreactors and managed to reduce costs and produce large quantities of ENs, ranging from 50,000 to 100,000 L in culture media. This approach is already used in Europe, North America, and Asia [ 57 ]. For the specific use of ENs in pest management programs, it should be noted that, although nematodes are easily produced both in vivo and in vitro in various complex semi-solid organic media, the cost of mass production using these methods represents a major constraint to their commercialization [ 57 ].…”

Section: En–fruit Fly Interactionmentioning

confidence: 99%

Can Entomopathogenic Nematodes and Their Symbiotic Bacteria Suppress Fruit Fly Pests? A Review

Toledo

Morán-Aceves²,

Ibarra

et al. 2023

Microorganisms

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Fruit flies (Diptera: Tephritidae) are serious pests that affect fruit production and marketing. Both third instar larvae and pupae are biological stages that persist in the soil until adult emergence. Entomopathogenic nematodes (ENs) are biological control agents that are used to control agricultural pests in greenhouse or field conditions. Several studies have been carried out under laboratory and field conditions showing how ENs can be applied within an area-wide integrated pest management approach to control fruit fly species in orchards and backyard fruit trees. In this review, we analyze how soil physical characteristics and biotic factors affect the performance of these biological control agents. Of the reviewed papers, more than half evaluated the influence of soil texture, humidity, temperature, and other factors on the performance of infective juveniles (IJs). Abiotic factors that significantly influence the performance of IJs are temperature, humidity, and texture. Among the biotic factors that affect IJs are fungi, bacteria, mites, insects, and earthworms. We conclude that ENs have the potential to be applied in the drip area of fruit trees that are infested by fruit flies and contribute to their suppression. This approach, in conjunction with an area-wide pest management approach, may contribute to pest suppression and increase the sustainability of agroecosystems.

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“…Contudo, a produção de galerias desses insetos em meio a dieta e fortes casulos, o custo de multiplicação e a fragilidade dos cadáveres são algumas desvantagens (MONTEIRO et al, 2014;DEVI et al, 2018).…”

Section: Produção In Vivounclassified

Scirtothrips dorsalis E PROSPECÇÃO DE SEU DESENVOLVIMENTO EM CONDIÇÃO TÉRMICA DE DOIS CÓRREGOS, SP

Pessoa¹,

Moriya²,

Mingoti³

et al. 2022

Entomologia: Estudos Sobre a Biodiversidade, Fisiologia, Controle E Importância Médica Dos Insetos 2

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Mass Production of Entomopathogenic Nematodes- A Review

Abstract: Abstract-Utilization of entomopathogenic nematodes (EPNs

Cited by 11 publications

References 50 publications

Mass production of the nematode <em>Acrobeloides longiuterus</em> using <em>Tribolium castaneum</em> and artificial solid media

Mass production of the nematode <em>Acrobeloides longiuterus</em> using <em>Tribolium castaneum</em> and artificial solid media

Can Entomopathogenic Nematodes and Their Symbiotic Bacteria Suppress Fruit Fly Pests? A Review

Scirtothrips dorsalis E PROSPECÇÃO DE SEU DESENVOLVIMENTO EM CONDIÇÃO TÉRMICA DE DOIS CÓRREGOS, SP

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