Mass production of Pinellia ternata multiple egg-shaped micro-tubers (MESMT) through optimized growth conditions for use in ethnomedicine

Kim, Yonggoo; Komakech, Richard; Choi, Ji Eun; Lee, Ka Youn; Lee, Tae Kyoung; Kim, Ki‐Hyun; Moon, Byeong Cheol; Kim, Suk Won; Kang, Young Min

doi:10.1007/s11240-019-01720-5

Cited by 8 publications

(6 citation statements)

References 42 publications

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“…Studies using plant hormones to increase biomass production or enlarge plant roots have also been performed for Polygoum multiflora, which is a herbal plant whose roots are also used in traditional medicine [33]. In addition, studies have been carried out on the thickening of roots by hormone processing and biomass enhancement based on sugar content [34]. Our results regarding the reproduction of R. glutinosa roots for three types of seedlings (SR, CR, and CS) are as follows.…”

Section: Discussionmentioning

confidence: 96%

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Verification of the Field Productivity of Rehmannia glutinosa (Gaertn.) DC. Developed Through Optimized In Vitro Culture Method

Kim

Komakech

Jeong

et al. 2020

Plants

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Rehmannia glutinosa (Gaertn.) DC is a perennial plant belonging to the family Scropulariidae. The root of R. glutinosa is used in oriental medicine and mainly grown using rootstock rather than seed cultivation, which gives rise to several problems including root rot, and results in a low productivity and poor quality. To solve the challenges involved in R. glutinosa seed cultivation, our team previously used the formative features and genetic analysis of R. glutinosa to determine the optimal in vitro tissue culture conditions for producing sterile culture seedlings and rootstocks of R. glutinosa. The aim of the present study was to identify differences between R. glutinosa standard rootstock seedlings (SR), R. glutinosa culture rootstock seedlings (CR), and culture seedlings (CS) under field conditions. The reproductive characteristics of the aerial part were more robust while the area and length of leaves were smaller for SR than those for CR and CS. The characteristic that differed the most in SR was flowering, which did not occur in CR and CS. In addition, the fresh and dry weights of the subterranean parts of CR and CS were two-fold greater than those of SR. Fourier transform near-infrared (FT-NIR) analysis showed only slight differences between the chemical constituents of SR and its culture products, which was confirmed by measuring the content of catalpol, an indexing substance. Catalpol had a reduced content in the culture products compared to SR. However, this difference was not significant. Our findings will be useful for the identification of the best seedling type of R. glutinosa to enable its mass production.

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Section: Discussionmentioning

confidence: 96%

“…These differences result from treatment with plant hormones. The adventitious root originates from mature cells that have re-acquired cell division activity within various tissues [33,34]. These meristematic cells develop into calyptrogen in a way that is similar to the development of cells in the lateral root.…”

Section: Discussionmentioning

confidence: 99%

Verification of the Field Productivity of Rehmannia glutinosa (Gaertn.) DC. Developed Through Optimized In Vitro Culture Method

Kim

Komakech

Jeong

et al. 2020

Plants

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“…There was no clear trend observed on the effects of root tuber biomass upon biochar inclusion for both CDG and lateritic soil. The growth in tuber biomass of P. ternata in soil (regardless of biochar application rate) is more than the growth of the same species in a chemical growth media (Kim et al, 2020). Only in case of biochar application in CDG, the tuber growth was more or comparable to genetically modified P. ternata reported by Juneidi et al (2020).…”

Section: Effects Of Biochar On Root Tuber Biomass Root Length and Tub...mentioning

confidence: 66%

Influence of biochar on improving hydrological and nutrient status of two decomposed soils for yield of medicinal plant - Pinellia ternata

Touyon

Bordoloi

2023

Journal of Hydrology and Hydromechanics

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The root tuber of Pinellia ternata has been used as a traditional therapeutic herbal medicine. It is reported to impart beneficial attributes in recovering COVID-19 patients. To meet an increasing demand of P. ternata, this study is intended to investigate the effects of biochar on the soil hydrological and agronomic properties of two decomposed soils (i.e., completely decomposed granite (CDG) and lateritic soil) for the growth of P. ternata. The plant was grown in instrumented pots with different biochar application rate (0%, 3% and 5%) for a period of three months. Peanut shell biochar inclusion in both soils resulted in reduction of soil hydraulic conductivity and increase in soil water retention capacity. These alterations in hydrological properties were attributed to measured change in total porosity, biochar intra pore and hydrophilic functional groups. The macro-nutrient (i.e., N, P, K, Ca, and Mg) concentration of both soils increased substantially, while the pH and cation exchange capacity levels in the amended soils were altered to facilitate optimum growth of P. ternata. The tuber biomass in biochar amended CDG at all amendment rate increases by up to 70%. In case of lateritic soil, the tuber biomass increased by 23% at only 5% biochar application rate. All treatments satisfied the minimum succinic acid concentration required as per pharmacopoeia standard index. The lower tuber biomass exhibits a higher succinic acid concentration regardless of the soil type used to grow P. ternata. The biochar improved the yield and quality of P. ternata in both soils.

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“…Therefore, a quantitative comparison of the underground and the aboveground biomasses was conducted. Most research on tissue culturing in Korea is related to the medicinal parts of Korean oriental medicine plants, which means that there has been previous research on root hypertrophy because it is mainly the roots that are used in traditional Korean medicine [ 21 , 22 ]. The results of the comparison between the SS and CS underground parts of P. multiflorum plants showed that the underground biomass of the CS samples was higher than that of the SS samples.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, sucrose is closely related to the development of food crop tubers [ 28 ]. Furthermore, the production of medicinal resources is related to the formation of tuber roots in Rehmannia glutinosa and Pinellia ternata [ 21 , 22 ]. Therefore, the formation of P. multiflorum tubers may also be partly owing to sucrose, and previous research on Korean oriental medicine plants [ 20 ] has suggested that this may explain why CS-derived plants have higher fresh weights than SS-derived plants.…”

Section: Discussionmentioning

confidence: 99%

Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (Polygonum multiflorum) Developed Using an In Vitro Culture Method

Kim

Komakech

Jeong

et al. 2020

Plants

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Polygonum multiflorum Thunb. is a perennial plant that belongs to Polygonaceae. Root tissues are the main plant parts used as medicinal herbs in Korean oriental medicine. The P. multiflorum tuber is well known for its medicinal properties in Korean oriental medicine, and it contains a number of useful substances (secondary metabolites of emodin, 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucoside (TSG), etc.) that are increasing in demand, as several studies show that they have beneficial effects on the human body. In this study, the production volumes and useful material content differences between cultured P. multiflorum seedlings (culture seedlings: CSs), which had been grown using a tissue culture technique under optimized conditions, and existing varieties in circulation (seed seedlings: SSs) were determined using a long-term field test. The growth characteristics of the underground parts were investigated by harvesting the tuberous roots (medicinal parts) after 1 year, and the results showed that the fresh and dry weights of the CS tubers were higher than those of the SS tubers. However, the SS rootlets had higher fresh and dry weights than the CS rootlets. A liquid chromatography-mass spectrometry component analysis of the P. multiflorum tubers and a Fourier transform near-infrared spectrophotometer analysis of the roots were undertaken. The results showed that the levels of TSG, which is a medicinal substance produced by P. multiflorum, were higher in the CSs than in the SSs, but the differences were not significant. The CS results from this study will inform future studies on the mass production of P. multiflorum in the field because the medicinal area was greater in CSs than in SSs.

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Mass production of Pinellia ternata multiple egg-shaped micro-tubers (MESMT) through optimized growth conditions for use in ethnomedicine

Cited by 8 publications

References 42 publications

Verification of the Field Productivity of Rehmannia glutinosa (Gaertn.) DC. Developed Through Optimized In Vitro Culture Method

Verification of the Field Productivity of Rehmannia glutinosa (Gaertn.) DC. Developed Through Optimized In Vitro Culture Method

Influence of biochar on improving hydrological and nutrient status of two decomposed soils for yield of medicinal plant - Pinellia ternata

Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (Polygonum multiflorum) Developed Using an In Vitro Culture Method

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