Mass production of potato shoots by liquid culture

김재훈,; 최은경,; 김정국,

doi:10.5010/jpb.2009.36.1.001

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…즉 조직배양 감자 줄기와 잎이 크고 굵으면 이들로부터 크고 튼실한 많은 수의 소 괴경를 얻을 수 있다 Hwang and Lee 2008). 김 등은 (Kim et al 2009) 액체배지가 들어 있는 소 형 유리바이오리액터 배양기에서 감자줄기를 대량으로 생산하는 방법을 보고하였다. 액체배지가 들어있는 바이 오리액터 배양기에서 감자 소괴경을 생산하는 방법은 기 본적으로 고체배지와 같다.…”

Section: 서 론unclassified

Mass production of potato microtubers by bioreactor culture

김재훈¹,

최은경²,

오승철³

et al. 2010

Journal of Plant Biotechnology

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Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek) shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and 20℃ were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then, greened microtubers were harvested after one week culture under light condition.

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Section: 서 론unclassified

Mass production of potato microtubers by bioreactor culture

김재훈¹,

최은경²,

오승철³

et al. 2010

Journal of Plant Biotechnology

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“…Boersig & Wagner (1988) suggested the use of aeroponics for seed potato production. South Korean studies (Kang et al, 1996;Kim et al, 1997;Chang et al, 2000) led to the transformation to mini-tuber production in solution culture. Studies were conducted in different countries, i.e., Spain (Muro et al, 1997;Farran & Mingo-Castel, 2006;Tierno et al, 2014), Belgium (Rolot & Seutin, 1999), Brazil (Corrêa et al, 2007), Peru (Mateus-Rodriguez et al, 2012), India (Bucksetha et al, 2016), and Kenya (Mbiyu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Pre-Basic Seed Potato Production in Egypt: Comparative Studies on Potato Nutrition in Aeroponics

Khalil,

Samy,

Abd El Halem

et al. 2023

Egypt. J. Bot.

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T HE PRODUCTION of potato pre-basic seed in aeroponic cultures was compared using three different nutrient solutions. Significant differences were recorded in the leaf content of elements P, K, Mg, Fe, B, Mn, and Zn, but not N or Ca, during vegetative growth. The differences between the main effects of the three tested nutrient solution formulas in minituber yield characteristics did not reach the significance level. However, varieties differed significantly in vegetative growth parameters, leaf content of elements, and mini-tuber yield. The Cara variety gave the highest large mini-tuber yield (>18 mm size) per plant in both seasons (20.5 and 27, respectively). Furthermore, the Cara variety yielded the highest total mini-tubers per plant in the second season (39.1). In contrast, in the first season, Cara and Hermes gave the highest significant total mini-tuber yield per plant (29.1 and 28.5, respectively). Diamant recorded the lowest values of mini-tuber number and weight per plant. The interaction between Cara and the second nutrient solution formula resulted in the highest number of large minitubers per plant in both seasons (25.4 and 33.4, respectively). The results support the aeroponic culture's use in the local production of pre-basic seed potato mini-tubers.

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“…기내 식물체의 뿌리 비대는 번식된 개체의 저장 및 운반이 양호하기 때 문에 또 다른 우수한 번식방법이 될 수 있다. 이러한 연 유로 감자에서는 뿌리 비대에 관한 다수의 연구 논문이 있으며 (Koda and Okazawa 1983;Khuri and Moorby 1995;1996;Donnelly et al, 2003), 이 기술을 이용한 대량번식으 로 실용화 되어 있다 (Kim et al 2009b). 기내 비대근 형성은 주로 단자엽 식물인 yam (Alizadeh et al 1998), Curcuma aromatica (Nayak 2000), Curcuma longa (Shirgurkar et al 2001)에서 보고되었고, 쌍자엽 식물에서 는 cassava (Medina et al 2007 형성율을 보였다 (Fig.…”

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Microtuber Formation from In Vitro Codonopsis lanceolata Plantlets by Sugar

Kim¹,

Moon²,

Choi³

2013

Journal of Plant Biotechnology

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In this experiment, we report for the first time mass propagation by in vitro mircrotuberization of Codonopsis lanceolata. We first examined the effect of cytokinins on multiple shoot induction. 2.0 mg·L -1 of kinetin not only gave the highest rate of shoot induction (19.1%) but also the elongation of shoot (17.1 mm). Secondly, we investigated the effect of sugars on in vitro microtuberization from nodal segments. The diameter of tuberous roots was enlarged in the half-strength MS medium supplemented with 145.9 mM sucrose. Histological analysis revealed that the number of parenchymatous cell containing starch grains increased in the tuberous roots. In addition, unlike in non-tuberous root, vascular bundles were scattered inner cortex layer. Thirdly, in order to preserve and stimulate the germination, microtubers were stored at 4℃ refrigerator during 9 months and then transplanted to the artificial soils (vermiculrite : peatmoss = 1:1 v/v), resulting that the rates of survival and germination were 75% and 70%, respectively. These results indicated that mass propagation of C. lanceolata was achieved by in vitro microtuber formation, suggesting that this protocol might be applied for not only the propagation of elite clones but also conservation of C. lanceolata germplasm.

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Mass production of potato shoots by liquid culture

Cited by 7 publications

References 23 publications

Mass production of potato microtubers by bioreactor culture

Mass production of potato microtubers by bioreactor culture

Pre-Basic Seed Potato Production in Egypt: Comparative Studies on Potato Nutrition in Aeroponics

Microtuber Formation from In Vitro Codonopsis lanceolata Plantlets by Sugar

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