Mass propagation and essential oil analysis of Artemisia vulgaris

Govindaraj, Sujatha; Kumari, B. D. Ranjitha; Cioni, Pier Luigi; Flamini, Guido

doi:10.1263/jbb.105.176

Cited by 51 publications

(25 citation statements)

References 20 publications

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“…The relative proportions of the components of the essential oil were derived from the normalised FID peak area, all relative response factors being taken as one. The chemical identity of each component was based on a match of its retention time with that of an authentic sample, comparing linear retention indices relative to the series of n-hydrocarbons, and on computer matching against commercial (NIST/EPA/NIH Mass Spectral Library) and home-made library mass spectra built up from pure substances and components of known oils and MS literature data (Chericoni et al 2004;Judzentiene and Buzelyte 2006;Govindaraj et al 2008). …”

Section: The Composition Of Essential Oilmentioning

confidence: 99%

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Production and characterisation of the intergeneric hybrids between Dendranthema morifolium and Artemisia vulgaris exhibiting enhanced resistance to chrysanthemum aphid (Macrosiphoniella sanbourni)

Deng

Chen

et al. 2009

Planta

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Aphids represent the most destructive of chrysanthemum pests to cultivation. Reliable variety sources of resistance and control methods are limited, so development of highly resistant breeding lines is desirable. An intergeneric hybrid between Dendranthema morifolium (chrysanthemum) variety 'Zhongshanjingui' and Artemisia vulgaris (mugwort) 'Variegata' was attempted. Most of the hybrid embryos aborted at an early developmental stage. Embryo rescue allowed the generation of hybrid plants, whose hybridity was confirmed by a combination of morphological, cytological and GISH analysis. The hybrids were vigorous, flowered normally, and their flower and leaf shape resembled those of the chrysanthemum more than those of the mugwort parent. The hybrids showed much higher resistance to chrysanthemum aphid (Macrosiphoniella sanbourni) than maternal chrysanthemum by inoculation test. The leaves of the hybrid developed a higher density of trichomes and secretory glands compared to the maternal chrysanthemum. GC-MS analysis revealed that approximately 51% of the essential oil in the hybrid leaves were monoterpenoids and sesquiterpenoids, while the proportion in the chrysanthemum was approximately 37%, and in the mugwort was approximately 90%. It is inferred that higher aphid resistance in the hybrid mainly owed to the leaf micromorphology and bioactive essential oil content.

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Section: The Composition Of Essential Oilmentioning

confidence: 99%

“…as major constituents (Judzentiene and Buzelyte 2006;Wang et al 2006;Govindaraj et al 2008). The aromatic perennial herb A. vulgaris 'Variegata' (''golden mugwort'') is a common ornamental plant in China, which is highly aphid-resistant (unpublished data), and thus represents a candidate source of improvement for chrysanthemum.…”

Section: Introductionmentioning

confidence: 99%

Production and characterisation of the intergeneric hybrids between Dendranthema morifolium and Artemisia vulgaris exhibiting enhanced resistance to chrysanthemum aphid (Macrosiphoniella sanbourni)

Deng

Chen

et al. 2009

Planta

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“…Camphor, eucalyptol, and terpinen-4-ol are major components of EOs from Artemisia feddei (Cha et al 2007), A. herba-alba (Mighri et al 2009), Artemisia haussknechtii (Jalali and Sereshti 2007), and Artemisia austriaca (Guvenalp et al 1998). Eucalyptol, germacrene D, camphor, and caryophyllene are the major compounds of EOs from A. annua (Goel et al, 2008;Padalia et al, 2011), A. vulgaris (Govindaraj et al 2008;Williams et al 2012), Artemisia verlotiorum (Chericoni et al 2004), and Artemisia parviflora (Rana et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Artemisia absinthium-borne compounds as novel larvicides: effectiveness against six mosquito vectors and acute toxicity on non-target aquatic organisms

Govindarajan

Benelli

2016

Parasitol Res

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The eco-friendly control of mosquito vectors is a crucial challenge of public health importance. Here we evaluated the larvicidal potential of Artemisia absinthium essential oil (EO) and its three major chemical constituents against six mosquito vectors: Anopheles stephensi, Anopheles subpictus, Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Culex tritaeniorhynchus. The EO was obtained by leaf hydrodistillation. Its chemical composition was analyzed using gas chromatography-mass spectrometry. Major components were (E)-β-farnesene (31.6 %), (Z)-en-yn-dicycloether (11.12 %), and (Z)-β-ocimene (27.8 %). The EO was toxic effect against larval populations of An. stephensi, An. subpictus, Ae. aegypti, A e . a l b o p i c t u s, C x . q u i n q u e f a s c i a t u s , and C x . tritaeniorhynchus, with LC 50 values of 41. 85, 52.02, 46.33, 57.57, 50.57, and 62.16 μg/ml. (E)-β-farnesene, (Z)-en-yndicycloether, and (Z)-β-ocimene were highly effective on An. stephensi (LC 50 = 8.13, 16.24 and 25.84 μg/ml) followed by An. subpictus (LC 50 = 10.18, 20.99, and 30.86 μg/ml), Ae. aegypti (LC 50 = 8.83,17.66, and 28.35 μg/ml), Ae. albopictus (LC 50 = 11.38,23.47, and 33.72 μg/ml), Cx. quinquefasciatus (LC 5 0 = 9.66, 19.76, and 31.52 μ g/ml), and Cx. tritaeniorhynchus (LC 50 = 12.51,25.88, and 37.13 μg/ml). Notably, the EO and its major compounds were safer to the non-target organisms Chironomous circumdatus, Anisops bouvieri and Gambusia affinis, with LC 50 values ranging from 207.22 to 4385 μg/ml. Overall, our results highlight that (E)-β-farnesene, (Z)-en-yn-dicycloether, and (Z)-β-ocimene from the A. absinthium EO represent promising eco-friendly larvicides against six key mosquito vectors with moderate toxicity against non-target organisms.

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“…Populations raised from seeds are characterised by morphological variation in plant height, leaf biomass and flowering dates, thus, resulting in low and variable yield of the active constituents (Elhag et al 1992). Micropropagation and organogenesis of different Artemisia species have been previously established by using several parts of plants, in order to obtain a large number of plants, such as A. scorpia (Aslam et al 2006), A. vulgaris L. (Govindaraj et al 2008), A. mutellina Vill. (Mazzetti and Donato 1998).…”

Section: Introductionmentioning

confidence: 99%

Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L.

Janarthanam

Rashmi²,

Sumathi³

2012

Plant Tissue Cult. & Biotech.

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An efficient plant regeneration protocol was developed for Artemisia annua, an aromatic medicinal herb. Nodal explants inoculated on MS supplemented with 4.44 μM BAP showed better growth response and produced 116.2 ± 0.1 microshoots of an average length 1.9 ± 0.3 cm after 35 days culture. The cluster of small shootlets were cultured on shoot elongation medium supplemented with 1.44 μM GA3 and 10% coconut milk (CM) showed shoot elongation up to 4.6 ± 0.7 cm. Roots were induced after transfer to half strength MS supplemented with 2.46 μM IBA produced 14.3 ± 0.2 roots with an average height of 4.3 ± 0.53 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 : 1 : 1).

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Mass propagation and essential oil analysis of Artemisia vulgaris

Cited by 51 publications

References 20 publications

Production and characterisation of the intergeneric hybrids between Dendranthema morifolium and Artemisia vulgaris exhibiting enhanced resistance to chrysanthemum aphid (Macrosiphoniella sanbourni)

Production and characterisation of the intergeneric hybrids between Dendranthema morifolium and Artemisia vulgaris exhibiting enhanced resistance to chrysanthemum aphid (Macrosiphoniella sanbourni)

Artemisia absinthium-borne compounds as novel larvicides: effectiveness against six mosquito vectors and acute toxicity on non-target aquatic organisms

Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L.

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