Mass Propagation of Strawberry and New Alternatives for Some Horticultural Crops

Boxus,

doi:10.1007/978-94-011-2785-1_9

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…In fact, after a first industrial application in Germany (Westphalen and Billen 1976), in about 10 years the annual production of micropropagated strawberry plants reached 7.5 million. This high-quality material, used as stock mother plants, produced a large part of the billion conventional strawberry plants produced annually in the world (Boxus 1992).…”

Section: Introductionmentioning

confidence: 99%

Comparing nursery behavior, field plant yield and fruit quality of in vitro and in vivo propagated strawberry mother plants

Capocasa

Balducci

Marcellini

et al. 2018

Plant Cell Tiss Organ Cult

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In different countries, mostly in EU, rules for strawberry nursery propagation impose the use of micropropagation only to produce stock virus free plant followed by at least three nursery production cycles in the field before selling these plants to growers. This limit has been imposed by problems concerning true-to-type plants observed in micro-propagated plants of some cultivars, with higher genotypic and phenotypic instability, and the incorrect use of higher concentrations of cytokinin in the proliferation phase. This production system of certified plants has high economic and environmental costs without a final guarantee on the sanitary state of the plants. New knowledges on in vitro techniques and new cultivars with higher genetic stability can offer the opportunity to improve the use of micropropagation in strawberry nursery industry. With the aim to propose a new protocol for strawberry propagation, an experimental trial was set up to compare the behavior of frigo and micro-mother plants of cv. Alba, a well know commercial cultivar in EU, in two cycles of nursery runner production and the yield, to verify in two successive cycles the fruit quality of daughter plants grown in open field conditions. The results confirmed the equivalence of the two types of mother plants both in plant nursery production and subsequently in field fruit production. Therefore, the direct use of micro-mother plants for nursery productions of frigo-plants for growers offer an alternative option for large scale frigo-plant nursery production.

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Section: Introductionmentioning

confidence: 99%

Comparing nursery behavior, field plant yield and fruit quality of in vitro and in vivo propagated strawberry mother plants

Capocasa

Balducci

Marcellini

et al. 2018

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Micro-propagation of strawberry has been applying in large scale for commercial production since mid1970s [12]. In vitro techniques had first applied to strawberry meristems to generate large scale of plantlets using an efficient method of mass propagation [11,13]. Strawberries can be propagated in vitro condition by tissue culture methods whereas micro-propagation is a very useful technique to get improved plantlets.…”

Section: Introductionmentioning

confidence: 99%

Clonal Propagation of Strawberry (Fragaria x ananassa Duch.) through In vitro Runner Tip Culture through Incorporation of Growth Hormones

Badal

Shoyeb

Sarkar

et al. 2019

BJI

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Runner tips explants of strawberry give rise to multiple shoots when cultured on MS medium supplemented with different concentrations and combinations of BAP with KIN or NAA or GA3.The highest response of shoot multiplication was obtained on MS containing 2.5 mgl-1 BAP + 0.5 mgl-1 Kin + 0.5 mgl-1 GA3. The maximum frequency of rooting (83%) and highest number of roots (3.49) was produced in medium containing 1.0 mgl-1 IBA. The well grown rooted plantlets were acclimatized and successfully established in autoclaved vermiculate soil and as well as natural condition. Using our established protocol, it is also possible to provide large numbers of micropropagated plantlets of this cultivars to produce high quality strawberry fruit for commercial cultivation practices.

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“…Strawberry (Fragaria ananassa Duch) is propagated conventionally through runners but it is not feasible for mass multiplication. Alternative methods of propagation like micropropagation have tremendous potential and attempts have been made for commercially exploitation (Boxus, 1974;Boxus et al, 1977). In the process of mass multiplication, the initial step of surface sterilization of explants is one of the most crucial factors in plant tissue culture.…”

Section: Introductionmentioning

confidence: 99%

Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler

Diengngan,

Murthy,

Mahadevamma

2014

J. Hortic. Sci.

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Nodal segments of strawberry cv. Chandler were collected from the field and surface sterilization of the explants was carried out using various levels of decontaminants as different treatments viz. sodium hypochlorite (NaClO) 0.5% for 7, 5 and 3 min and mercuric chloride (Hg Cl2) 0.1% for 5 min, 3 min and 3 min 10 sec. The explants were excised and cultured for 3 weeks on the initiation medium supplemented with various levels of BAP (0.5, 1, 1.5 and 2mg/l) and its combination with GA (0.5 and 1mg/l). Sub culturing was done and finally rooting was initiated on the medium supplemented with various levels of IBA (0.5, 1, 1.5 and 2mg/l) for 4 weeks. The results obtained indicated that, among the decontaminants, treatment of explants with Hg Cl2 0.1% for 3 min 10 sec resulted in the minimum contamination and browning of explants. Further, maximum shoot proliferation percentage, shoots per explant and minimum number of days to shoot initiation was observed when explants were cultured on MS medium supplemented with 1.5 mg/l BAP over other concentrations of either BAP or in combination with GA. However, the maximum length of shoots was obtained in medium supplemented with 1.5 mg/l BAP + 0.5 mg/l GA. While, medium supplemented with 0.5 mg/l IBA supported highest percentage of rooting, the highest number of roots, maximum root length of microcuttings and minimum number of days to root initiation were observed in medium supplemented with 1mg/l of IBA.

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Mass Propagation of Strawberry and New Alternatives for Some Horticultural Crops

Cited by 7 publications

References 18 publications

Comparing nursery behavior, field plant yield and fruit quality of in vitro and in vivo propagated strawberry mother plants

Comparing nursery behavior, field plant yield and fruit quality of in vitro and in vivo propagated strawberry mother plants

Clonal Propagation of Strawberry (Fragaria x ananassa Duch.) through In vitro Runner Tip Culture through Incorporation of Growth Hormones

Effective Decontamination and Regeneration Protocol for in vitro Culture of Strawberry Cv. Chandler

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