Mass propagation through direct and indirect organogenesis in three species of genus Zephyranthes and ploidy assessment of regenerants through flow cytometry

Syeed, Rukaya; Mujib, A.; Malik, Moien Qadir; Mamgain, Jyoti; Ejaz, Bushra; Gulzar, Basit; Zafar, Nadia

doi:10.1007/s11033-020-06083-1

Cited by 26 publications

(13 citation statements)

References 45 publications

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“…In addition, compared with TDZ and NAA, suitable KT concentration showed more shoots per explants during the adventitious buds induction of Korean pine, and above or below the optimal dose of KT did not show any improvement in parameters of adventitious buds, which is in line with previous studies that exceeding optimal KT levels inhibits bud proliferation (Akbaş et al 2009;Arab et al 2014). TDZ is a phenyl urea-type cytokinin that mimics the activity of both auxin and cytokinin, which are the most preferably used cytokinins in some woody plant regeneration systems (Dewir et al 2018;Rukaya et al 2021). Previous studies described TDZ was used solely for indirect organogenesis and proved more effective for the shoot induction process in a range of plants in vitro (Khanam et al 2018;Wu et al 2020;Syeed et al 2021).…”

Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantssupporting

confidence: 85%

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

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The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was successfully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg • L −1 kinetin (KT) in combination 0.5 mg • L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg • L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg • L −1 ), and activated charcoal (AC; 1 g • L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg•L −1 NAA and 0.5 or 1 mg • L −1 indole-3-butyric acid (IBA).Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.

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Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantssupporting

confidence: 85%

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantssupporting

confidence: 85%

“…TDZ is a phenyl urea-type cytokinin that mimics the activity of both auxin and cytokinin, which are the most preferably used cytokinins in some woody plant regeneration systems (Dewir et al 2018;Rukaya et al 2021). Previous studies described TDZ was used solely for indirect organogenesis and proved more effective for the shoot induction process in a range of plants in vitro (Khanam et al 2018;Wu et al 2020;Syeed et al 2021). However, in our study, TDZ in combination with KT was more conducive to the direct adventitious buds formation of Korean pine, which can be explained by the fact that TDZ is highly stable and regulates endogenous hormones, and shows the activity of both auxins as well as cytokinin, similarly mentioned in the literature (Dewir et al 2018;Dey et al 2012) also found that TDZ appears to stimulate cells in the apical meristem to divide, multiply, and develop so that bud differentiation occurred.…”

Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantsmentioning

confidence: 99%

Direct in Vitro Organogenesis From Sprouted Seeds of a Highly Economical and Ecological Valued Tree, Korean Pine

Liang

Bai

et al. 2021

Preprint

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The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was success fully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg · L −1 kinetin (KT) in combination 0.5 mg · L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg · L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg · L −1 ), and activated charcoal (AC; 1 g · L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg·L −1 NAA and 0.5 or 1 mg · L −1 indole-3-butyric acid (IBA). Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.

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“…The structure of the mature embryo sac in Z. candida is described and the behaviours of the synergids, the central cell and the antipodal cells are discussed (Ao et al, 20020). Mass propagation through direct and indirect organogenesis in three species of genus Zephyranthes and ploidy assessment of regenerants through flow cytometry were studied (Syeed et al, 2021). Effects of leaf area index and degree of canopy cover of green turf and ground cover plants on rainwater interception in Z. candida was examined by Zhang et al (2018).…”

Section: Introductionmentioning

confidence: 99%

Micromorphology and anatomy of the flower of Zephyranthes candida (Amaryllidaceae)

Fishchuk

2021

Regul. Mech. Biosyst.

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The use of morphological features of flowers in the taxonomy of plants is becoming increasingly important. The structure of the Zephyranthes candida (Lindl.) Herb. flowers on permanent cross-sectional and longitudinal sections was studied using a light microscope. The genus Zephyranthes belongs to the subtribe Hippeastrinae Walp. tribe Hippeastreae Sweet., family Amaryllidaceae s.l. Microscopic studies of the flower are considered as a tool to identify hitherto unknown structural adaptations of plants to specialized pollination methods and to elucidate the first stages of fruit morphogenesis, as many features of the fruit appear at the flower stage. The morphometric parameters, morphology, anatomy, and vascular anatomy of the ovary were described by using the flower’s transverse sections. Ten flowers of Z. candida were sectioned using standard methods of Paraplast embedding and serial sectioning at 20 μm thickness. Sections were stained with Safranin and Astra Blau and mounted in Eukitt. It was found that in the studied species the tepals have multi-bundle traces of 10–12 leading bundles. We consider the gynoecium of the studied species to be eusincarpous. The vascular system of the inferior ovary consists of three dorsal and three septal bundles, paired ventral bundles of carpels, which form ovule traces. For the first time, the presence of the following gynoecium zones was detected: a synascidiate structural zone with a height of about 360 μm and a fertile symplicate structural zone with a height of about 1560 μm and a hemisymplicate zone of 480 μm. Septal nectaries appear in the hemisymplicate zone and open with nectary split at the base of the style, the total height of the septal nectary is 760 μm. The ovary roof is 280 μm. Bifurcated dorsal and septal bundles of carpels have been identified, which can be considered as adaptations of the early stages of fruit morphogenesis to opening. Anatomical features of the ovary of Z. candida are numerous vascular bundles in the pericarpium, non-lignified endocarp at the flower stage, we consider as adaptations to the formation of juicy fruit. New data on the anatomical structure of the flower are a significant addition to the information on antecological and post-anthetic features of the studied species. Also, these data can be used in the construction of parsimony branches of the family Amaryllidaceae.

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Mass propagation through direct and indirect organogenesis in three species of genus Zephyranthes and ploidy assessment of regenerants through flow cytometry

Cited by 26 publications

References 45 publications

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Direct in Vitro Organogenesis From Sprouted Seeds of a Highly Economical and Ecological Valued Tree, Korean Pine

Micromorphology and anatomy of the flower of Zephyranthes candida (Amaryllidaceae)

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