Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge

Breillatt, J.P.; Brantley, J. N.; Mazzone, H.M.; Martignoni, Mauro E.; Franklin, J. E.; Anderson, Norman G.

doi:10.1128/aem.23.5.923-930.1972

Applied Microbiology

1972

DOI: 10.1128/aem.23.5.923-930.1972

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Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge

J.P. Breillatt¹,

J. N. Brantley²,

H.M. Mazzone³

et al.

Abstract: Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a continuous-sample-flow-with-isopycnic-banding centrifuge in quantities up to 6 x 1013 polyhedral inclusion bodies per day. Continuousflow methods for S-p type purification have been evolved to deal with mass isolation of bioparticles.

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1978

1984

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Cited by 17 publications

References 7 publications

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Comparative properties of the inclusion body proteins of the nucleopolyhedrosis viruses ofNeodiprion sertifer andLymantria dispar

Brown

Kaczmarek

Dubois

et al. 1979

Archives of Virology

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A comparison was made on the properties of the inclusion body proteins of two insect viruses: the nucleopolyhedrosis viruses of the European pine sawfly, Neodiprion sertifer, Geoffroy, and the gypsy moth, Lymantria dispar, Linnaeus. The inclusion body proteins were characterized by the following parameters: amino acid composition, polyacrylamide gel electrophoresis in the absence and presence of sodium dodecyl sulfate--mercaptoethanol, isoelectric focusing, and alkaline protease activity. The properties of the inclusion body proteins of the two viruses were similar in many respects, but clear differences were observed. A principal difference was the absence of alkaline protease activity associated with the inclusion body proteins of N. sertifer nucleopolyhedrosis virus.

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Comparative properties of the inclusion body proteins of the nucleopolyhedrosis viruses ofNeodiprion sertifer andLymantria dispar

Brown

Kaczmarek

Dubois

et al. 1979

Archives of Virology

Self Cite

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Effects ofBlepharipa pratensis [Dip.: Tachinidae] on the pathogenicity of nucleopolyhedrosis virus in stage V ofLymantria dispar [Lep.: Lymantriidae]

Godwin

Shields

1984

Entomophaga

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The effects of myiasis caused by Blepharipa pratensis (Meigen) on the pathogenicity of 3 dosages (1.00 • 104 PIB, 3.75 • 104 PIB and 7.50 x 104 PIB) of nucleopolyhedrosis virus (NPV) in stage V of Lymantria dispar (L.) were tested. When fed only NPV, 44 % of the larvae fed the low dosage died, 67 % of the larvae fed the mid-level dosage died, and 73 % of those fed the high-level dosage died. At the low dosage, mortality was significantly lower than at the other dosages. The presence of the parasite significantly increased mortality due to NPV ; 65 % of the larvae fed the low dosage died, 77 % of the larvae fed the mid-level dosage died, and 80 % of the larvae fed the highlevel dosage died. For biological control, the combination of NPV and parasite would increase mortality, but at the expense of the parasite.Although nucleopolyhedrosis is considered to be the prime cause of collapse of dense populations of Lymantria dispar (L.) (Campbell & Podgwaite, 1971), the processes that initiate epizooties and the mechanisms whereby nucleopolyhedrosis virus (NPV) spreads through the host community are not well understood. Because entomophagous parasites have an intimate association with juvenile L. dispar, and move about freely in the host community, they may be important in the epizootiology of nucleopolyhedrosis. Reardon & Podgwaite (1976) found positive correlations between the incidence of NPV and the incidence ofParasetigena silvestris (Robineau-Desvoidy) andApanteles melanoscelus (Ratzeburg) in natural populations ofL. dispar. Under laboratory conditions A. melanoscelus has been found capable of transmitting NPV (Raimo et aL, 1977). We have shown that the.presence ofBlepharipa pratensis (Meigen) in stage IV larvae enhanced deaths to NPV by about 38.7 % (Godwin & Shields, 1982).B. pratensis is thought to be the most successful insect parasite of L. dispar (PsehornWaleher, 1974 ; Angalet, 1974 (1)). It lays a microtype egg on leaves, and the egg must be swallowed for myiasis to be initiated. The number of the eggs on leaves increases as the season progresses ; late instars eat proportionately more eggs than do young larvae ; and the parasite (1) G

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Some interactions ofSerratia marcescens nucleopolyhedrosis virus andBlepharipa pratensis [Dip.: Tachinidae] inLymantria dispar [Lep.: Lymantriidae]

Godwin

Shields

1982

Entomophaga

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The entomopathogens Serratia marcescens Btzm and nucleopolyhedrosis virus were each fed alone and in combination with the parasite Blepharipa pratensis (MEIGEN) tO 4th-instar gypsy moth, Lymantria dispar, (L.) larvae.At LD30 for NPV, the presence of the parasite enhanced polyhedrosis about 30 ~ but the total number of gypsy moth larvae and pupae killed (85 %) was not significantly different from the number killed by the parasite alone (93 %). When the parasite was combined with S. marcescens, a strain nonpathogenic in L. dispar, total mortality was not significantly different from that in insects exposed only to the parasite (89 and 86 %, respectively), but parasite survival was reduced about 12 %. However, deaths not attributable to the parasite could not be ascribed to the bacterium either.

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Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge

Cited by 17 publications

References 7 publications

Comparative properties of the inclusion body proteins of the nucleopolyhedrosis viruses ofNeodiprion sertifer andLymantria dispar

Comparative properties of the inclusion body proteins of the nucleopolyhedrosis viruses ofNeodiprion sertifer andLymantria dispar

Effects ofBlepharipa pratensis [Dip.: Tachinidae] on the pathogenicity of nucleopolyhedrosis virus in stage V ofLymantria dispar [Lep.: Lymantriidae]

Some interactions ofSerratia marcescens nucleopolyhedrosis virus andBlepharipa pratensis [Dip.: Tachinidae] inLymantria dispar [Lep.: Lymantriidae]

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