Early sowing increases yield and avoid stresses, but require cold tolerance. A limited number of cold tolerant maize genotypes have been reported. The objective of this study was to identify broad 脳 narrow genetic base combinations as breeding populations for improving cold tolerance. Nine cold tolerant populations were crossed to five inbred lines, and evaluated in cold chamber and in the field. None of the maize inbred lines or populations was entirely cold tolerant. None of the inbred 脳 population combinations fulfil all requirements for early sowings. The crosses between the inbred line EP80 and northwestern Spanish populations are the most promising base germplasm for further breeding programs for cold tolerance. Particularly, EP80 脳 Puenteareas showed the largest yield and good performance at first stages of development under cold conditions, either in cold chamber and field. The cross EP80 脳 Rebordanes had better performance at first stages of development under cold conditions, but poorer agronomic characteristics at early planting. In addition, EP80 and Puenteareas showed the most favorable GCA for most traits. Early vigor would be the most suitable trait to select maize genotypes with superior cold tolerance during emergence and post-emergence stages, as it was the only trait for which differences among genotypes were observed in both the cold chamber and the field.