Mass spectral studies of some fructose containing oligosaccharide permethyl ethers

Das, Kalpataru; Thayumanavan, B.

doi:10.1002/oms.1210061002

Cited by 17 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mass spectra obtained for lactose and sucrose agreed with previously published results [17,18]. The mass spectrum of the permethylated alditol of the A-trisaccharide (Fig.…”

Section: Discussionsupporting

confidence: 89%

“…The identification of lactose, sucrose, and the three blood-group-active oligosaccharides, was based on identical retention times and mass spectra as compared to those of authentic samples. The mass spectra of lactose and sucrose have been published previously [17,18]. The retention time of the A-trisaccharide was 1.48 relative to maltotriose and its mass spectrum and some important primary fragments are given in Fig.…”

Section: Quantitation Of Disaccharides and Trisaccharides In Regions mentioning

confidence: 99%

See 1 more Smart Citation

Urinary Excretion of Oligosaccharides Induced by Galactose Given Orally or Intravenously

Chester¹,

Hallgren²,

Lundblad³

et al. 1979

Eur J Biochem

View full text Add to dashboard Cite

The effect of oral administration of galactose, lactose, and sucrose and intravenous injection of galactose on the urinary excretion of blood-group-active oligosaccharides has been studied. Galactose given either as the free sugar, a glycoside (lactose) or a constituent of normal diet was an absolute requirement for the formation and excretion of A-trisaccharide, B-trisaccharide and 2'-fucosylgalactose in blood group A, B and O(H) secretors, respectively. Great individual variation was seen in the amounts of galactose-dependent oligosaccharides excreted. Injection of galactose resulted in excretion of 3 -59 of the amount of oligosaccharide formed after oral administration to the same individual. The mean ratio A-trisaccharide/B-trisaccharide was 2.7 in four blood-group-AIB secretors and 0.22 in three A2B secretors and can thus serve as a parameter for chemical differentiation between the two blood groups. The excretion of larger blood-group-active oligosaccharides, including the A-pentasaccharide, the B-pentasaccharide and lactodifucotetraose, that are normal components in urine from, respectively, starved A, B, and H secretors, was about the same after oral administration of galactose or lactose. The B-trisaccharide wdS the only oligosaccharide detected in plasma after oral galactose administration to a blood-group-B secretor individual. The concentration was 0.38 mg/l of plasma.Blood-group-active tetrasaccharides and pentasaccharides have been isolated from the urine of starved blood-group A, B or H secretors [l -41. Normal food intake or lactose diet induced an excretion of additional blood-group-active disaccharides or trisaccharides in these urines [5]. The oligosaccharides formed by A, B and H secretors are shown in Fig.

show abstract

“…The mass spectra obtained for lactose and sucrose agreed with previously published results [17,18]. The mass spectrum of the permethylated alditol of the A-trisaccharide (Fig.…”

Section: Discussionsupporting

confidence: 89%

Section: Quantitation Of Disaccharides and Trisaccharides In Regions mentioning

confidence: 99%

Urinary Excretion of Oligosaccharides Induced by Galactose Given Orally or Intravenously

Chester¹,

Hallgren²,

Lundblad³

et al. 1979

Eur J Biochem

View full text Add to dashboard Cite

show abstract

“…83 Similar decomposition processes, along with cleavage of glycosidic bonds, are typical of permethylated oligosaccharides. 84 CI mass spectra of permethylated carbohydrates show small [M + H] + peaks, the decomposition of which is due to a successive loss of two molecules of CH 3 OH. 85 Sometimes, alkylation can lead to new fragmentation pathways under EI.…”

Section: Some Mass Spectral Features Of Alkyl (Aryl) Derivativesmentioning

confidence: 99%

Derivatization in Mass Spectrometry– 3. Alkylation (Arylation)

Halket

Заикин

2004

Eur J Mass Spectrom (Chichester)

View full text Add to dashboard Cite

The review is devoted to alkylation (arylation) as a widely employed derivatization procedure for the protection of OH (carboxylic acids, phosphoric acids, sulfonic acids, alcohols, polyols, phenols, enols), SH (thiols) and NH (amines, amides) groups in order to increase volatility, to improve the chromatographic properties and, if possible, mass spectral properties of derivatives. Chemical aspects of derivatization and various alkylation (arylation) reagents and reaction procedures are described. Specific mass spectral (electron ionization, chemical ionization) features of derivatives helpful in identification, structure elucidation, profiling and quantitative determination of the above-mentioned polar compounds by coupled gas chromatography or high-performance liquid chromatography are discussed. Some common analytical applications of the procedures in organic chemistry, clinical chemistry, environmental chemistry etc. are briefly summarized.

show abstract

“…,~ who demonstrated the discrimination between (1-2), (1)(2)(3)(4) and (1)(2)(3)(4)(5)(6) linkages. The analysis of permethyl saccharides containing fructose as one of the constituents was reported by Das and Thayumanavan,' whereas Karkkainen6 has discussed the mass spectrometry of permethyl trisaccharides.…”

Section: Introductionmentioning

confidence: 99%