Mass Spectrometric Analysis of Food Bioactive Oligosaccharides

Hernández‐Hernández, Oswaldo; Roepstorff, Peter

doi:10.1002/9781118817360.ch23

Cited by 7 publications

(3 citation statements)

References 56 publications

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“…A review on mass spectrometric analysis of bioactive oligosaccharides from food has been published (Hernández‐Hernández & Roepstorff, ).…”

Section: Applications To Food Analysismentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Harvey

2018

Mass Spectrometry Reviews

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This review is the eighth update of the original article published in 1999 on the application of Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2014. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, and arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly- saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2018 Wiley Periodicals, Inc. Mass Spec Rev 37:353-491, 2018.

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“…A review on mass spectrometric analysis of bioactive oligosaccharides from food has been published (Hernández‐Hernández & Roepstorff, ).…”

Section: Applications To Food Analysismentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Harvey

2018

Mass Spectrometry Reviews

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“…Briefly, there are currently a wide range of commercially available mass analysers that differ in sensitivity, resolution, dynamic range, mass accuracy and fragmentation behaviour (Sprenger and Roepstorff, 2012). The most commonly used in oligosaccharide studies are quadrupole (Q), linear ion trap (LIT/LTQ), quadrupole ion trap (QIT), time of flight (TOF) and Orbitrap, as well as tandem systems composed of two or more coupled analysers of the same or different types, such as QqQ, Q-TOF, QqQ-LIT (Hernández-Hernández and Roepstorff, 2014). In addition, recent advances have led to the use of novel type of mass spectrometers to oligosaccharides analysis, as it is the case of ion mobility mass spectrometry (IMMS) which is based on ions separation from their mass/charge, but also from their size and shape.…”

Section: Mass Spectrometrymentioning

confidence: 99%

Structure and function of non-digestible carbohydrates in the gut microbiome

Rastall

Díez-Municio

Forssten³

et al. 2022

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Together with proteins and fats, carbohydrates are one of the macronutrients in the human diet. Digestible carbohydrates, such as starch, starch-based products, sucrose, lactose, glucose and some sugar alcohols and unusual (and fairly rare) α-linked glucans, directly provide us with energy while other carbohydrates including high molecular weight polysaccharides, mainly from plant cell walls, provide us with dietary fibre. Carbohydrates which are efficiently digested in the small intestine are not available in appreciable quantities to act as substrates for gut bacteria. Some oligo- and polysaccharides, many of which are also dietary fibres, are resistant to digestion in the small intestines and enter the colon where they provide substrates for the complex bacterial ecosystem that resides there. This review will focus on these non-digestible carbohydrates (NDC) and examine their impact on the gut microbiota and their physiological impact. Of particular focus will be the potential of non-digestible carbohydrates to act as prebiotics, but the review will also evaluate direct effects of NDC on human cells and systems.

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“…However, incomplete chromatographic separation of tetrasaccharides and use of positive mode of ESI-MS characterization of trisaccharides hampered elucidation of structures of all obtained isomers. Our study presents improvement in elucidation of structure of obtained oligosaccharides using a method that does not require previous chromatographic separation of different GOS and has been recognized as a productive and efficient method in the analysis of food bioactive oligosaccharides. − Also, we have monitored products during the reaction course, not just at the peak of GOS synthesis, as was the case in previous studies, to obtain important findings about enzyme affinity toward the formation of different linkages in different reaction stages.…”

Section: Introductionmentioning

confidence: 99%

Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry–Tandem Mass Spectrometry

Carević

Bezbradica

Banjanac

et al. 2016

J. Agric. Food Chem.

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Galacto-oligosaccharides (GOS) represent a diverse group of well-characterized prebiotic ingredients derived from lactose in a reaction catalyzed with β-galactosidases. Enzymatic transgalactosylation results in a mixture of compounds of various degrees of polymerization and types of linkages. Because structure plays an important role in terms of prebiotic activity, it is of crucial importance to provide an insight into the mechanism of transgalactosylation reaction and occurrence of different types of β-linkages during GOS synthesis. Our study proved that a novel one-step method, based on ion-mobility spectrometry-tandem mass spectrometry (IMS-MS/MS), enables complete elucidation of GOS structure. It has been shown that β-galactosidase from Aspergillus oryzae has the highest affinity toward formation of β-(1→3) or β-(1→6) linkages. Additionally, it was observed that the occurrence of different linkages varies during the reaction course, indicating that tailoring favorable GOS structures with improved prebiotic activity can be achieved by adequate control of enzymatic synthesis.

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Mass Spectrometric Analysis of Food Bioactive Oligosaccharides

Cited by 7 publications

References 56 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Structure and function of non-digestible carbohydrates in the gut microbiome

Structural Elucidation of Enzymatically Synthesized Galacto-oligosaccharides Using Ion-Mobility Spectrometry–Tandem Mass Spectrometry

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