Mass spectrometric analysis of <i>in vitro</i>
 nuclear aggregates of polyamines

Picariello, Gianluca; Iacomino, Giuseppe; Luccia, Aldo Di; D’Agostino, Luciano

doi:10.1002/rcm.6807

Cited by 6 publications

(8 citation statements)

References 27 publications

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“…Anionic complexes of the form (H n-1 AO m -) 2 (8)(9)(10)(11)(12)(13)(14) were obtained after removing one proton from each monomer in 1-7 and optimizing the geometry. Adding one Na + to each monomer in 8-14 and optimizing the geometry, neutral salt complexes of type (NaH n-1 AO m ) 2 were obtained (15)(16)(17)(18)(19)(20)(21).…”

Section: Methodsmentioning

confidence: 99%

The Paradox of Hydrogen-Bonded Anion–Anion Aggregates in Oxoanions: A Fundamental Electrostatic Problem Explained in Terms of Electrophilic···Nucleophilic Interactions

et al. 2014

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A theoretical study of anionic complexes formed by two partly deprotonated oxoacids joined by hydrogen bonds has been carried out at the MP2 computational level. In spite of the ionic repulsion, local energy minima are found both in the gas phase and in aqueous solution. Electrostatic potential and electron density topologies, and the comparison with neutral complexes formed by oxoacids, reveal that the ionization has no significant effect on the properties of the hydrogen bonds. The stability of the complexes in the gas phase is explained by attractive forces localized in a volume situated in the hydrogen bond and defined as the electrostatic attraction region (EAR) and determined by the topological analyses of the electron density and the electrostatic potential, and by the electric field lines. In solution, the strong anionic repulsion is mostly screened by the effect of the surrounding polar solvent, which only leads to a weak destabilizing interaction in the hydrogen bond region and finally favors the overall stability of the complexes. The anion−anion complexes have been compared with the corresponding neutral ones (as salts or protonated forms), showing that EAR remains unchanged along the series.

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Section: Methodsmentioning

confidence: 99%

The Paradox of Hydrogen-Bonded Anion–Anion Aggregates in Oxoanions: A Fundamental Electrostatic Problem Explained in Terms of Electrophilic···Nucleophilic Interactions

et al. 2014

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“…In the central tract, the DNA is naked and some s-NAP debris, due to the nanotube damage produced, probably, by the cantilever passage, can be perceived (11). 9 and evidenced that NAPs also mediate the interaction of the double strands with HMGB1, a DNA binding protein (10,11,(13)(14)(15)…”

Section: Nuclear Aggregates Of Polyaminesmentioning

confidence: 99%

“…Protein-DNA super-aggregation is permitted by the interaction of the positive charges of the proteins and the external NAPs' phosphates. In fact, the outer surface of the nanotubes has a charge asset utilizable for protein interaction, due to the presence of phosphates-phosphate complexes (13,28,29) (Figure 4).…”

Section: Nap Functional Outcomementioning

confidence: 99%

“…MALDI-TOF MS signals of s-ivNAP and their possible assignment (only the external face of s-NAP is represented). Spm: spermine; Spd: spermidine (13).…”

Section: Figurementioning

confidence: 99%

“…Therefore, a role of electronics in DNA physiology cannot be acknowledged, if one limits its consideration to the setting of the bases. On the other hand, another "aromatic" environment is present onto the strands: in native conditions, they are enveloped by a polymeric system of nanotubes formed by the self-assembly of polyamines and phosphate in cyclic structures -the Nuclear Aggregates of Polyamines (NAPs) (6)(7)(8)(9)(10)(11)(12)(13)(14)(15) -that I suggest here as a powerful electronic apparatus that assists the DNA in its functions. This paper has the aim of interpreting the ascertained morphological and functional characteristics of NAPs at the light of the basic principles of the electronics, and hence to 4 delineate their possible role as electronic structures acting in still debated fields of cell nucleus physiology.…”

Section: Introductionmentioning

confidence: 99%

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Native DNA electronics: the Nuclear Aggregates of Polyamines possible role

D’Agostino¹

2018

Preprint

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The genomic DNA is enveloped by nanotubes formed by the Nuclear Aggregates of Polyamines (NAPs) that induce DNA conformational changes, and provides protection and increased interactive abilities for the double strands. In a physiologic environment, the nanotube arrangement initiates with the spontaneous interaction among the terminal amino groups of polyamines and the phosphate ions, with the consequent formation of cyclic monomers that hook at DNA grooves. The polymer thus formed has the morphological features of an organic semiconductor and, therefore, can be considered able to conduce electric charges. Phosphate ions positioned on NAP external surface could regulate, as in a physical electric circuit, both protein linear and rotational (histones) motion, in accordance with the basilar principles of the electronics. A model of a carrier system for proteins motion along the polymer wrapping the DNA strands, based on the phosphate-phosphate complexation, is proposed.

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