Mass spectrometric analysis of lipid species of human circulating blood cells

Leidl, Katharina; Liebisch, Gerhard; Richter, Dorothea; Schmitz, Gerd

doi:10.1016/j.bbalip.2008.07.008

Cited by 147 publications

(111 citation statements)

References 45 publications

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“…This way lipid species profiles from lipoprotein fractions [74], blood cells [76] and tissues signatures [77] may be expanded and could help to understand the species heterogeneity and origin of bioactive lipid species. Furthermore, metabolic profiling of the lipid metabolism with stable isotope labeled precursors could be a valuable tool to substantiate this knowledge [78][79][80].…”

Section: Discussionmentioning

confidence: 99%

Quantification of bioactive sphingo- and glycerophospholipid species by electrospray ionization tandem mass spectrometry in blood

Liebisch

Scherer

2012

Journal of Chromatography B

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Section: Discussionmentioning

confidence: 99%

Quantification of bioactive sphingo- and glycerophospholipid species by electrospray ionization tandem mass spectrometry in blood

Liebisch

Scherer

2012

Journal of Chromatography B

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“…The use of the PC head group is rationalized as (i) conclusions on fatty acid impact requires a constant head group behavior; (ii) NMR studies on GpA have been conducted in DPC micelles or DMPC/DHPC bicelles [22]; and (iii) PC is one of the most abundant head group in the plasma membrane of erythrocytes [17,18]. The analysis of the different fatty acids was prompted by the observation that the dimer of “serine zipper” peptides is more stable in thick bilayers (POPC) when compared to thin bilayers (DLPC) or micelles [38].…”

Section: Discussionmentioning

confidence: 99%

“…This membrane consists of phospholipids, cholesterol and glycolipids. Among the phospholipids PC, phosphatidylethanolamine, and phosphatidylserine are most abundant and the fatty acids thereof have a length of 16 to 22 carbon atoms [17,18]. GpA carries an O - and N -glycan, which again carries sialic acid.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

Flinner¹,

Mirus²,

Schleiff³

2014

IJMS

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The hydrophobic thickness of membranes, which is manly defined by fatty acids, influences the packing of transmembrane domains of proteins and thus can modulate the activity of these proteins. We analyzed the dynamics of the dimerization of Glycophorin A (GpA) by molecular dynamics simulations to describe the fatty acid dependence of the transmembrane region assembly. GpA represents a well-established model for dimerization of single transmembrane helices containing a GxxxG motif in vitro and in silico. We performed simulations of the dynamics of the NMR-derived dimer as well as self-assembly simulations of monomers in membranes composed of different fatty acid chains and monitored the formed interfaces and their transitions. The observed dimeric interfaces, which also include the one known from NMR, are highly dynamic and converted into each other. The frequency of interface formation and the preferred transitions between interfaces similar to the interface observed by NMR analysis strongly depend on the fatty acid used to build the membrane. Molecular dynamic simulations after adaptation of the helix topology parameters to better represent NMR derived structures of single transmembrane helices yielded an enhanced occurrence of the interface determined by NMR in molecular dynamics simulations. Taken together we give insights into the influence of fatty acids and helix conformation on the dynamics of the transmembrane domain of GpA.

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“…Rapid interconversion of sphingolipid metabolites mediate signaling cascades with myriad cell-specific effects. Mammalian cells and tissues contain very low concentrations of the two major free sphingoid bases (sphingosine (4-sphingenine), 10–20 pmol/mg; dihydrosphingosine (sphinganine), 1–5 pmol/mg), whereas their phosphorylated forms (sphingosine 1-phosphate (S1P), 0.31 μM in serum; dihydrosphingosine 1-phosphate (dhS1P), 0.04 μM in serum) are more abundant, especially in the blood/ lymph [146–148]. Sphingoid bases are absorbed from various dietary sources, but in mammals, the majority of spingolipids appear to be synthesized endogenously (Fig.…”

Section: Sphingolipidsmentioning

confidence: 99%

Lipid Mediators of Allergic Disease: Pathways, Treatments, and Emerging Therapeutic Targets

Schauberger

Peinhaupt

Cazares

et al. 2016

Curr Allergy Asthma Rep

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Bioactive lipids are critical regulators of inflammation. Over the last 75 years, these diverse compounds have emerged as clinically-relevant mediators of allergic disease pathophysiology. Animal and human studies have demonstrated the importance of lipid mediators in the development of asthma, allergic rhinitis, urticaria, anaphylaxis, atopic dermatitis, and food allergy. Lipids are critical participants in cell signaling events which influence key physiologic (bronchoconstriction) and immune phenomena (degranulation, chemotaxis, sensitization). Lipid-mediated cellular mechanisms including: (1) formation of structural support platforms (lipid rafts) for receptor signaling complexes, (2) activation of a diverse family of G-protein coupled receptors, and (3) mediating intracellular signaling cascades by acting as second messengers. Here, we review four classes of bioactive lipids (platelet activating factor, the leukotrienes, the prostanoids, and the sphingolipids) with special emphasis on lipid synthesis pathways and signaling, atopic disease pathology, and the ongoing development of atopy treatments targeting lipid mediator pathways.

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Mass spectrometric analysis of lipid species of human circulating blood cells

Cited by 147 publications

References 45 publications

Quantification of bioactive sphingo- and glycerophospholipid species by electrospray ionization tandem mass spectrometry in blood

Quantification of bioactive sphingo- and glycerophospholipid species by electrospray ionization tandem mass spectrometry in blood

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

Lipid Mediators of Allergic Disease: Pathways, Treatments, and Emerging Therapeutic Targets

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