Mass spectrometric characterization of elements and molecules in cell cultures and tissues

Arlinghaus, Heinrich F.; Kriegeskotte, C.; Fartmann, M.; Wittig, Andrea; Sauerwein, W.; Lipinsky, D.

doi:10.1016/j.apsusc.2006.02.186

Cited by 40 publications

(35 citation statements)

References 6 publications

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“…Ion-induced electron images were acquired from all samples by detecting the electrons produced by the scanning primary ion beam to select regions of interest for further analysis and to check the topography of the sample surface (1,21). For each region of analysis, ion images for K, Na, P, Ca, 10 B, and additional molecular fragments were acquired.…”

Section: Animals and Tumorsmentioning

confidence: 99%

“…The application of imaging techniques, such as mass spectrometry, provides an excellent tool to guide decisions already in the initial stages of drug development. Laser postionization secondary neutral mass spectrometry (laser-SNMS) can simultaneously detect atoms, their isotopes and molecules (through well defined small fragments), in tissue with high sensitivity and with microscopic spatial resolution (1). The principal advantage of this technology is the ability to directly observe the distribution of elements and molecules without specific labeling by fluorescent or radioactive probes.…”

Section: Introductionmentioning

confidence: 99%

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Laser postionization secondary neutral mass spectrometry in tissue: a powerful tool for elemental and molecular imaging in the development of targeted drugs

Wittig¹,

Arlinghaus

Kriegeskotte

et al. 2008

Molecular Cancer Therapeutics

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The exact intracellular localization and distribution of molecules and elements becomes increasingly important for the development of targeted therapies and contrast agents. We show that laser postionization secondary neutral mass spectrometry (laser-SNMS) is well suited to localize particular elements and small molecules with subcellular spatial resolution applying the technique exemplary to Boron Neutron Capture Therapy (BNCT). We showed in a murine sarcoma that the drugs used for clinical BNCT, namely L-para-boronophenylalanine (700 mg/kg body weight i.p.) and sodium mercaptoundecahydrocloso-dodecaborate (200 mg/kg body weight i.p.), transport the therapeutic agent 10 B into the cytoplasm and into the nucleus itself, the most sensitive area of the cell. Sodium mercaptoundecahydro-closo-dodecaborate distributes 10 B homogeneously and L-para-boronophenylalanine heterogeneously. When combining laser-SNMS with prompt ;-ray analysis as a screening technique, strategies for BNCT can be elaborated to develop new drugs or to improve the use of existing drugs on scientifically based evidence. The study shows the power of laser-SNMS in the early stages of drug development, also outside BNCT.

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Section: Animals and Tumorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Laser postionization secondary neutral mass spectrometry in tissue: a powerful tool for elemental and molecular imaging in the development of targeted drugs

Wittig¹,

Arlinghaus

Kriegeskotte

et al. 2008

Molecular Cancer Therapeutics

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show abstract

“…7 and 8). 133 In this case, a finely focused primary ion beam sweeps the sample in a raster pattern and, simultaneously, the secondary ion intensities are saved as a function of beam position. Furthermore, SIMS has been successfully applied for isotopic measurements in nuclear astrophysics applications.…”

mentioning

confidence: 99%

Critical revision of GD-MS, LA-ICP-MS and SIMS as inorganic mass spectrometric techniques for direct solid analysis

Pisonero

Fernández

Günther

2009

J. Anal. At. Spectrom.

158

115

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“…Interesting recent examples include three dimensional imaging of oocytes [14], relative quantification of cholesterol in cell membranes [15], and distinguishing cancerous cells of differing breast cancer phenotypes [16]. Cellular imaging has also been shown by a variety of other mass spectrometric techniques, including desorption/ionization on silicon [17] and laser post-ionization secondary neutral mass spectrometry [18].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Single Cells for Imaging Mass Spectrometry

Berman¹,

Fortson²,

Kulp

2010

Methods in Molecular Biology

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Characterizing chemical changes within single cells is important for determining fundamental mechanisms of biological processes that will lead to new biological insights and improved disease understanding. Imaging biological systems with mass spectrometry (MS) has gained popularity in recent years as a method for creating precise chemical maps of biological samples.In order to obtain high-quality mass spectral images that provide relevant molecular information about individual cells, samples must be prepared so that salts and other cell-culture components are removed from the cell surface and the cell contents are rendered accessible to the desorption beam. We have designed a cellular preparation protocol for imaging MS that preserves the cellular contents for investigation and removes the majority of the interfering species from the extracellular matrix. Using this method, we obtain excellent imaging results and reproducibility in three diverse cell types: MCF7 human breast cancer cells, Madin-Darby canine kidney (MDCK) cells, and NIH/3T3 mouse fibroblasts. This preparation technique allows routine imaging MS analysis of cultured cells, allowing for any number of experiments aimed at furthering scientific understanding of molecular processes within individual cells.

show abstract

Mass spectrometric characterization of elements and molecules in cell cultures and tissues

Cited by 40 publications

References 6 publications

Laser postionization secondary neutral mass spectrometry in tissue: a powerful tool for elemental and molecular imaging in the development of targeted drugs

Laser postionization secondary neutral mass spectrometry in tissue: a powerful tool for elemental and molecular imaging in the development of targeted drugs

Critical revision of GD-MS, LA-ICP-MS and SIMS as inorganic mass spectrometric techniques for direct solid analysis

Preparation of Single Cells for Imaging Mass Spectrometry

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