Mass spectrometric determination of gases in plants

Langer, G.A.; Bohátka, S.; Berecz, I.; Schlenk, B.; Bornemisza-Pauspertl, P.; Kiss, Klara; Buzás, I.; Pártay, Géza; Mózsik, L.; Sági, F.

doi:10.1016/0042-207x(84)90323-3

Cited by 5 publications

(2 citation statements)

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“…22 The latter method has been realized in our laboratory and it has offered new possibilities for in-vivo monitoring of plants. 16,23 Other methods measure in a closed volume around the plant or some of its leaves. The MIMS method, however, makes an intimate contact between the plant and the analyser.…”

Section: In-vivo Measurements In Plantsmentioning

confidence: 99%

Process Monitoring in Fermentors and Living Plants by Membrane Inlet Mass Spectrometry

Bohátka

1997

Rapid Commun. Mass Spectrom.

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Mass spectrometers offer multicomponent analysis, and higher sensitivity and speed compared with conventional sensors. Mass spectrometric measurements in liquid media need an interface and membrane inlets have proved very flexible and convenient for this purpose. This new method was originally introduced by Hoch and Kok who used it to study photosynthesis and respiration.1 When one side of a membrane is located in a liquid and the other is in vacuum, gases dissolved in the liquid diffuse into the membrane and are released on the vacuum side. The amount of the pervaporated gas is proportional to its partial pressure on the highpressure side of the membrane. In many cases of interest, the analytes are gases and volatiles dissolved in liquids. Well chosen membrane inlets are selectively good conductors for gases while retaining the bulk of water away from the mass spectrometer.2-4 Teflon or silicone rubber foils and tubes are preferred. Water in an organic solvent can also be measured using other membranes. 5 It was shown early 6 and later explained [7][8][9] how sensitive the response time of the system is to the dimensions of the connecting tube, which the sampled gases and volatiles have to traverse in vacuum to the mass spectrometer ion source. Adsorption-desorption on surfaces between the membrane and the mass analyser contributes dominantly to the transients and response time where vapours are also present. The characteristics of the membrane and the mass spectrometer jointly determine the sensitivity of the measurement. Most systems use quadrupole or magnetic sector mass spectrometers. Membrane inlet mass spectrometry (MIMS) has been widely used in blood-gas measurements, biotechnology, environmental and analytical applications. [10][11][12] After the basic innovations, the history of MIMS systems is mainly that of a series of different approaches to the coupling between the membrane and the mass spectrometer. For a general description we refer to other reviews and papers. 4,[13][14][15] We have constructed different types of quadrupole mass spectrometers (QMS) covering the mass range of 1-800 u and turned our attention, among other applications, towards biotechnology, biology and ecology. 6,16,17 Our answers to the problems arising in the construction and use of MIMS systems in fermentation systems, as well as some unique applications in biology and ecology, are presented below.

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Section: In-vivo Measurements In Plantsmentioning

confidence: 99%

Process Monitoring in Fermentors and Living Plants by Membrane Inlet Mass Spectrometry

Bohátka

1997

Rapid Commun. Mass Spectrom.

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“…More recently non-invasive transeutaneous measurement of oxygen and helium dissolved in arterial blood using a specially designed membrane probe was described 40) Weaver ~30) gave a review on the medical and other applications of MS for measurement of volatiles. Langer et al 65) measured dissolved gases in plants.…”

Section: Present State Of Measurement Technique and Trendsmentioning

confidence: 99%