Mass spectrometric determination of prostanoids in rat hypothalamic paraventricular nucleus microdialysates

Tachi, Masahiko; Kondo, Fumio; Fukayama, Minoru; Yoshikawa, Kazuhiro; Matsuura, Keiichi; Okada, Shoshiro

doi:10.1016/j.autneu.2013.12.013

Cited by 7 publications

(8 citation statements)

References 27 publications

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“…These modifications achieved a baseline separation of six (Fig. 1b) and provided almost the same sensitivity as observed in a previous report [8].…”

Section: Optimization Of Analytical Conditions For Neuroactive Substasupporting

confidence: 74%

“…and GLP-1 (3 nmol/animal, i.c.v.) increased TxB 2 levels [8]; therefore, we used the same doses in the present experiments. The changes in levels of neurotransmitters and prostanoids by CRF and GLP-1 administration are shown in subpanels a and b of Fig.…”

Section: Determination Of Neurotransmitters and Prostanoids In The MImentioning

confidence: 98%

“…In the present study, two major prostanoids, 6-keto-PGF 1α (a stable metabolite of PGI 2 ) and PGF 2α , were analyzed in addition to four prostanoids (TxB 2 , PGE 2 , PGD 2 , 15d-PGJ 2 ) that were measured in our previous report [8]. To selectively and sensitively determine six prostanoids, HPLC conditions were slightly modified and MS scans were split into five segments as described in the BMaterials and methods^section.…”

Section: Optimization Of Analytical Conditions For Neuroactive Substamentioning

confidence: 99%

“…Analysis of sample fractions can be carried out using liquid chromatography (LC)-electrochemical detection, capillary electrophoresislaser induced fluorescence detection, immunoassay, and, more recently, LC-tandem mass spectrometry [1]. Recently, we reported a stable isotope-dilution liquid chromatography-ion trap tandem mass spectrometry (LC-ITMS n ) method for simultaneous quantification of four prostanoids, TxB 2 , PGE 2 , PGD 2 , and 15-deoxy-Δ 12,14 (15d)-PGJ 2 , in the microdialysates from rat hypothalamic paraventricular nucleus (PVN), one of the most important autonomic control centers in the brain [8]. This method was successfully applied to the analysis of prostanoids present at nanomolar levels and to the measurement of prostanoid concentrations in rat PVN in response to the excitatory amino acid N-methyl-D-aspartate (NMDA) and the stress-related peptides corticotrophin-releasing factor (CRF) and glucagon-like peptide-1 (GLP-1).…”

Section: Introductionmentioning

confidence: 99%

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Changes in hypothalamic neurotransmitter and prostanoid levels in response to NMDA, CRF, and GLP-1 stimulation

et al. 2015

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Determination of neuroactive substances, such as neurotransmitters and prostanoids, in the extracellular space of the living brain is a very important technique in neuroscience. The hypothalamic paraventricular nucleus (PVN) is one of the most important autonomic control centers in the brain. Recently, we demonstrated that thromboxane (Tx) A2 in the PVN may play an important role in adrenomedullary outflow evoked by N-methyl-D-aspartate (NMDA), corticotrophin-releasing factor (CRF), and glucagon-like peptide-1 (GLP-1) stimulation using microdialysis sampling and liquid chromatography-ion trap tandem mass spectrometry (LC-ITMS(n)). In the present study, we investigated whether centrally administered NMDA, CRF, and GLP-1 can release five neurotransmitters, acetylcholine (ACh), histamine, glutamate (Glu), γ-aminobutyric acid (GABA), and serotonin (5-HT), along with six prostanoids, TxB2, prostaglandin (PG) E2, PGD2, 15-deoxy-∆(12,14) (15d)-PGJ2, 6-keto-PGF1α, and PGF2α in rat PVN microdialysates after optimization of LC-ITMS(n) conditions . All stimulations increased the levels of 5-HT, TxB2, PGE2, and PGF2α (except for 5-HT stimulated with GLP-1). Only NMDA increased the levels of ACh, Glu, and GABA. Treatment with dizocilpine maleate (MK-801), a noncompetitive NMDA receptor antagonist, attenuated the NMDA-induced increase in the levels of neuroactive substances except for Glu. This is the first study to use LC-ITMS(n) to analyze neurotransmitters and prostanoids in the same microdialysates from rat PVN.

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“…These modifications achieved a baseline separation of six (Fig. 1b) and provided almost the same sensitivity as observed in a previous report [8].…”

Section: Optimization Of Analytical Conditions For Neuroactive Substasupporting

confidence: 74%

Section: Determination Of Neurotransmitters and Prostanoids In The MImentioning

confidence: 98%

Section: Optimization Of Analytical Conditions For Neuroactive Substamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Changes in hypothalamic neurotransmitter and prostanoid levels in response to NMDA, CRF, and GLP-1 stimulation

et al. 2015

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“…GC-MS analysis was performed according to a previous study (Yamada et al, 2013 The combined organic phases were then evaporated to dryness in vacuo to give the residues, which were individually dissolved in 100 L of acetonitrile. Aliquots (10 μL) of these stock solutions were analyzed with a previously described method (Tachi et al, 2014). In brief, 40 μL of prepared samples were spiked with 10 μL of the internal standard mixture containing 10 ng/mL 15d-PGJ2-d4 for calibration.…”

Section: Transparent Methodsmentioning

confidence: 99%

Clostridium butyricum Modulates the Microbiome to Protect Intestinal Barrier Function in Mice with Antibiotic-Induced Dysbiosis

et al. 2020

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Clostridium butyricum MIYAIRI 588 (CBM 588) is a probiotic bacterium that has previously been used to prevent antibiotic-associated diarrhea. However, the underlying mechanism by which CBM 588 protects the gut epithelial barrier remains unclear. Here, we show that CBM 588 increased the abundance of Bifidobacterium, Lactobacillus, and Lactococcus species in the gut microbiome and also enhanced the intestinal barrier function of mice with antibiotic-induced dysbiosis. Additionally, CBM 588 significantly promoted the expansion of IL-17A-producing gdT cells and IL-17A-producing CD4 cells in the colonic lamina propria (cLP), which was closely associated with changes in the intestinal microbial composition. Additionally, CBM 588 plays an important role in controlling antibiotic-induced gut inflammation through upregulation of anti-inflammatory lipid metabolites such as palmitoleic acid, 15d-prostaglandin J 2 , and protectin D 1 . This study reveals a previously unrecognized mechanism of CBM 588 and provides new insights into gut epithelial barrier protection with probiotics under conditions of antibiotic-induced dysbiosis.

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Centrally administered isoproterenol induces sympathetic outflow via brain prostaglandin E2-mediated mechanisms in rats

Ando¹,

Kondo²,

Yamaguchi³

et al. 2015

Autonomic Neuroscience

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Mass spectrometric determination of prostanoids in rat hypothalamic paraventricular nucleus microdialysates

Cited by 7 publications

References 27 publications

Changes in hypothalamic neurotransmitter and prostanoid levels in response to NMDA, CRF, and GLP-1 stimulation

Changes in hypothalamic neurotransmitter and prostanoid levels in response to NMDA, CRF, and GLP-1 stimulation

Clostridium butyricum Modulates the Microbiome to Protect Intestinal Barrier Function in Mice with Antibiotic-Induced Dysbiosis

Centrally administered isoproterenol induces sympathetic outflow via brain prostaglandin E2-mediated mechanisms in rats

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