Mass spectrometric identification of candidate RNA-binding proteins associated with Transition Nuclear Protein mRNA in the mouse testis

Phillips, Bart; Williams, Jason; Atchley, Dustin T.; Xu, Xiaojiang; Li, Jianliang; Adams, Andrea L.; Johnson, Katina L.; Hall, Traci M. Tanaka

doi:10.1038/s41598-019-50052-z

Cited by 8 publications

(7 citation statements)

References 43 publications

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“…Embryonic Lethal Abnormal Vision (ELAV) L1 is one such candidate RBP pulled down by H1T2 antibody which is reported as a key regulator of posttranscriptional regulation in spermatocytes, whose targeted deletion results in male sterility phenotype [ 80 ]. More recent studies have supported the mRNA regulatory role of Elavl1 by specifically associating to Tnp mRNAs to control the delayed timing of their translation [ 81 ]. H1T2–chromatin IP/MS analysis also identified YbX2, another potential mRNA repressor involved in the regulation of translation whose knockout resulted in male infertility and azoospermia condition [ 82 ].…”

Section: Resultsmentioning

confidence: 99%

Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids

Vasantha

Bhaduri

Ravikkumar

et al. 2021

Epigenetics & Chromatin

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Background H1T2/H1FNT is a germ cell-specific linker histone variant expressed during spermiogenesis specifically in round and elongating spermatids. Infertile phenotype of homozygous H1T2 mutant male mice revealed the essential function of H1T2 for the DNA condensation and histone-to-protamine replacement in spermiogenesis. However, the mechanism by which H1T2 imparts the inherent polarity within spermatid nucleus including the additional protein partners and the genomic domains occupied by this linker histone are unknown. Results Sequence analysis revealed the presence of Walker motif, SR domains and putative coiled-coil domains in the C-terminal domain of rat H1T2 protein. Genome-wide occupancy analysis using highly specific antibody against the CTD of H1T2 demonstrated the binding of H1T2 to the LINE L1 repeat elements and to a significant percentage of the genic regions (promoter-TSS, exons and introns) of the rat spermatid genome. Immunoprecipitation followed by mass spectrometry analysis revealed the open chromatin architecture of H1T2 occupied chromatin encompassing the H4 acetylation and other histone PTMs characteristic of transcriptionally active chromatin. In addition, the present study has identified the interacting protein partners of H1T2-associated chromatin mainly as nucleo-skeleton components, RNA-binding proteins and chaperones. Conclusions Linker histone H1T2 possesses unique domain architecture which can account for the specific functions associated with chromatin remodeling events facilitating the initiation of histone to transition proteins/protamine transition in the polar apical spermatid genome. Our results directly establish the unique function of H1T2 in nuclear shaping associated with spermiogenesis by mediating the interaction between chromatin and nucleo-skeleton, positioning the epigenetically specialized chromatin domains involved in transcription coupled histone replacement initiation towards the apical pole of round/elongating spermatids.

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Section: Resultsmentioning

confidence: 99%

Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids

Vasantha

Bhaduri

Ravikkumar

et al. 2021

Epigenetics & Chromatin

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“…In addition to mito‐nuclear genes, over‐dominant genes in Italian sparrow contained ribonucleoproteins present in both cytosolic and mitochondrial ribosomes. RNA binding proteins play an important role in post‐translational regulation of gene expression and spermatogenesis and appear to be key regulatory factors that ensure male fertility (Paronetto & Sette, 2010; Phillips et al, 2019). Several of the over‐dominant genes also coded for subunits of T‐complex protein Ring Complex (TRiC) involved in folding of about 10% of the proteome.…”

Section: Discussionmentioning

confidence: 99%

Extensive transgressive gene expression in testis but not ovary in the homoploid hybrid Italian sparrow

et al. 2022

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“…In addition to mito-nuclear genes, over-dominant genes in Italian sparrow contained ribonucleoproteins present in both cytosolic and mitochondrial ribosomes. RNA binding proteins play an important role in post-translational regulation of gene expression and spermatogenesis and appear to be key regulatory factors that ensure male fertility (Paronetto & Sette, 2010;Phillips et al, 2019). Several of the over-dominant genes also coded for subunits of T-complex protein Ring Complex (TRiC) involved in folding of about 10% of the proteome.…”

Section: Discussionmentioning

confidence: 99%

Extensive transgressive gene expression in testis but not ovary in the homoploid hybrid Italian sparrow

Yazdi

Rowe

Ravinet³

et al. 2021

Preprint

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Hybridization is increasingly recognized as an important evolutionary force contributing novel variation for selection to act on. While mis-expression in F1 hybrids is well documented, how gene expression evolves in stabilized hybrid taxa and contributes novel variation remains an open question, especially for hybrid species without an increase in ploidy. As gene expression evolves in a stabilizing manner, break-up of co-evolved cis- and trans-regulatory elements could lead to transgressive patterns of gene expression in hybrids. Here, we address to what extent gonad gene expression has evolved in an old homoploid hybrid, Italian sparrow, Passer italiae. Through comparing the gene expression of parental species and F1 hybrids to that of the Italian sparrow, we find evidence for strongly transgressive expression in the Italian sparrow, with 22% of the testis genes exhibiting expression patterns outside the range of both parent species, compared to only 0.37% in the F1s. In contrast, Italian sparrow ovary expression was similar to that of one parent species, the house sparrow (P. domesticus). Moreover, the Italian sparrow testis transcriptome is 26.2 -26.6 times as diverged from those of the parent species compared to how divergent their transcriptomes are in spite of it being genetically intermediate. This highlights the potential for regulation of gene expression to produce novel variation following hybridization. Genes involved in energy production and protein synthesis are enriched in the subset that is over-dominantly expressed in Italian sparrow testis, suggesting that selection on key functions have molded the hybrid transcriptome.

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Mass spectrometric identification of candidate RNA-binding proteins associated with Transition Nuclear Protein mRNA in the mouse testis

Cited by 8 publications

References 43 publications

Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids

Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids

Extensive transgressive gene expression in testis but not ovary in the homoploid hybrid Italian sparrow

Extensive transgressive gene expression in testis but not ovary in the homoploid hybrid Italian sparrow

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