Mass spectrometric phosphoproteome analysis of small-sized samples of human neutrophils

C5a impairs phagosomal maturation in the neutrophil through phosphoproteomic remodeling

“…To our knowledge, the data presented here ( Figure 3 , Figure 4 , and Figure 5 ) represent the deepest assessment of the human neutrophil proteome and phosphoproteome ( 49 – 51 ). These data provide a phosphoproteomic assessment of the human neutrophil response to S .…”

Proteomic profile of platelets during reconstitution of platelet counts after apheresis

“…LC‐ESI‐MS/MS measurements were conducted as described . In brief, for each sample 4 μg of protein were digested using Trypsin and desalted via ZipTip.…”

The formation and function of the neutrophil phagosome

“…The C5a‐mediated reduction in acidification was associated with a reduced phosphorylation of the V‐ATPase G subunit. Defective phagosomal acidification was also demonstrated in neutrophils from critically ill patients vulnerable to secondary infection, suggesting that acquired phagosome dysfunction in the context of systemic inflammation may increase susceptibility to infection 24 . Another interesting observation is that when neutrophils phagocytose other apoptotic neutrophils, for example at sites of acute inflammation where neutrophils significantly outnumber macrophages, their oxidative burst is reduced with a resultant acidification of the phagosome, although pH was again measured at later timepoints (90 minutes) 171 .…”