Mass spectrometric profiling of (neuro)-peptides in the worker honeybee, Apis mellifera

Boerjan, Bart; Cardoen, Dries; Bogaerts, Annelies; Landuyt, Bart; Schoofs, Liliane; Verleyen, Peter

doi:10.1016/j.neuropharm.2009.06.026

Cited by 62 publications

(64 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neuropeptide-like precursor 1 (NPLP1) is conserved in various insects and contains multiple mono-and dibasic cleavage sites for the processing of several peptides, either bioactive neuropeptides or spacer peptides [29,30,95,96]. Currently, a functional role for only one NPLP-1 derived peptide has been elucidated.…”

Section: Neuropeptide-like Precursormentioning

confidence: 99%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

Self Cite

View full text Add to dashboard Cite

Abstract. Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/ pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects.

show abstract

Section: Neuropeptide-like Precursormentioning

confidence: 99%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nevertheless, our RNA sequencing experiments do show that the neuropeptide genes corazonin, NVPIYQEPRF-containing and LRNQLDIGDLQ-containing were differentially expressed in the PI of JH-treated bees. These neuropeptides have been localized to the corpora cardiaca and corpora allata (Boerjan et al, 2010), indicating that these neuropeptides may act as neurohormones in honey bees. In addition, our analyses were able to detect a robust overlapping signal between the behavioral maturation and JHA experiments, for both differentially expressed genes in general and transcription factor motifs.…”

Section: Discussionmentioning

confidence: 99%

Diet and endocrine effects on behavioral maturation-related gene expression in the pars intercerebralis of the honey bee brain

Wheeler

Ament

Rodriguez-Zas

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

Nervous and neuroendocrine systems mediate environmental conditions to control a variety of life history traits. Our goal was to provide mechanistic insights as to how neurosecretory signals mediate division of labor in the honey bee (Apis mellifera). Worker division of labor is based on a process of behavioral maturation by individual bees, which involves performing in-hive tasks early in adulthood, then transitioning to foraging for food outside the hive. Social and nutritional cues converge on endocrine factors to regulate behavioral maturation, but whether neurosecretory systems are central to this process is not known. To explore this, we performed transcriptomic profiling of a neurosecretory region of the brain, the pars intercerebralis (PI). We first compared PI transcriptional profiles for bees performing in-hive tasks and bees engaged in foraging. Using these results as a baseline, we then performed manipulative experiments to test whether the PI is responsive to dietary changes and/or changes in juvenile hormone (JH) levels. Results reveal a robust molecular signature of behavioral maturation in the PI, with a subset of gene expression changes consistent with changes elicited by JH treatment. In contrast, dietary changes did not induce transcriptomic changes in the PI consistent with behavioral maturation or JH treatment. Based on these results, we propose a new verbal model of the regulation of division of labor in honey bees in which the relationship between diet and nutritional physiology is attenuated, and in its place is a relationship between social signals and nutritional physiology that is mediated by JH.

show abstract

“…Global gene expression, microarrays, and quantitative polymerase chain reaction (PCR) can be applied to measure tissue specific gene expression under a variety of conditions. These approaches have recently uncovered the FGLa/ASTs from the Hymenoptera, including the honey bee (Apis mellifera L., 1758) (Hummon et al 2006;Boerjan et al 2010), the parasitoid wasp Nasonia vitripennis (Walker, 1836) (Hauser et al 2010), and the Panamanian leafcutter ant (Acromyrmex echinatior (Forel, 1899)) (GenBank accession No. EGI57352); Hemiptera, including the pea aphid (Acrythosiphon pisum Harris, 1776) (Huybrechts et al 2010) and R. prolixus (Ons et al 2009(Ons et al , 2011; and Siphunculata, the human body louse (Pediculus humanus corporis) (cited in Weaver and Audsley 2009).…”

Section: The Fglamide-related-ast Familymentioning

confidence: 99%

“…The six amino acid spacing between the Trp residues is still maintained (Ons et al 2011). The Hymenoptera A. mellifera and N. vitropennis appear to lack MIPs, as peptide sequences could not be detected by isolation or through genome mining (Hummon et al 2006;Boerjan et al 2010;Hauser et al 2010). It was noted that the honey-bee equivalent of the D. melanogaster MIP receptor was detected in the honey-bee genome, which casts doubt on the absence of a ligand (Boerjan et al 2010).…”

Section: The Fglamide-related-ast Familymentioning

confidence: 99%

Families of allatoregulator sequences: a 2011 perspective¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

BendenaWilliam

TobeStephen

2012

Can. J. Zool.

View full text Add to dashboard Cite

Three different peptide families have been named "allatostatins" (ASTs), based on their initial purifications which were based on their ability to inhibit juvenile hormone (JH) biosynthesis. These include (i) a family of peptides that have a consensus C-terminal sequence Y/FXFGL-NH 2 ; (ii) a family of peptides with a conserved C-terminal sequence W(X) 6 W-NH 2 ; and(iii) a family of peptides with C-terminal sequence PISCF, some of which are C-terminally-amidated. Each allatostatin family has functions distinct and apart from the inhibition of JH biosynthesis. A peptide family known as the "allatotropins" serve to stimulate JH biosynthesis. This family of peptides also has been proven to exert multiple effects dependent on the species in question. Genome and peptidome projects are uncovering new members of these families and it is clear that these structures are not just confined to Insecta but are found in a range of invertebrates. The receptors for these neuropeptides have been identified and tested experimentally for specific ligand binding. The Y/FXFGLa-ASTs exert their action through galanin-like receptors, W(X) 6 Wa-ASTs through a sex peptide-binding receptor, and PISCF-ASTs through somatostatin-like receptors. These receptors are conserved through evolutionary time and are being identified in numerous invertebrates by way of genome projects.Key words: allatostatin, allatotropin, juvenile hormone, muscle contraction, neuropeptides, neuropeptide receptors, insects, crustaceans, arthropods.Résumé : Trois familles différentes de peptides portent le nom d'« allatostatines » (AST) d'après leurs purifications initiales basées sur leur capacité d'inhiber la biosynthèse de l'hormone juvénile (JH). Elles incluent (i) une famille de peptides qui possèdent une séquence consensus C-terminale Y/FXFGL-NH 2 , (ii) une famille de peptides avec une séquence C-terminale conservée W(X) 6 W-NH 2 et (iii) une famille de peptides avec une séquence C-terminale PISCF, dont certains sont amidatées sur l'extrémité C-terminale. Chaque famille d'allatostatines possède des fonctions distinctes et additionnelles à l'inhibition de la synthèse de l'hormone juvénile. Une famille de peptides connus sous le nom d'« allatotropines » sert à stimuler la synthèse de la JH. Cette famille de peptides produit aussi des effets multiples selon l'espèce concernée. Des projets sur le génome et le peptidome découvrent de nouveaux membres de ces familles et il est clair que ces structures ne se retrouvent pas seulement chez les insectes, mais aussi chez une gamme d'invertébrés. Les récepteurs de ces neuropeptides ont été identifiés et ont été testé expérimentalement pour des liaisons spécifiques au ligand. Les AST-Y/FXFGLa exercent leur action par des récepteurs de type galanine, les AST-W(X)6Wa par un récepteur liant un peptide sexuel et les AST-PISCF par des récepteurs de type somatostatine. Ces récepteurs sont conservés au cours de l'évolution et sont présentement retrouvés chez de nombreux invertébrés dans le cadre des projets de génome.

show abstract

Mass spectrometric profiling of (neuro)-peptides in the worker honeybee, Apis mellifera

Cited by 62 publications

References 43 publications

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Diet and endocrine effects on behavioral maturation-related gene expression in the pars intercerebralis of the honey bee brain

Contact Info

Product

Resources

About