Mass spectrometrical analysis of cuticular proteins from the wing of Hebemoia glaucippe (Linnaeus, 1758) (Lepidoptera: Pieridae)

Bae, Narkhyun; Lödl, Martin; Pollak, Arnold; Lubec, Gert

doi:10.1016/j.jprot.2011.08.017

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…In previous studies, CPG genes have been identified in Bombyx mori (Bombycidae) and characterized by their high glycine contents that primarily result from the repeats GGYGG and GGxGG. In Bombyx mori, CPGs contribute to larval, pupal and adult cuticles in combination with other cuticle proteins and compose the adult trachea in the wing 13 15 16 . Three glycine-rich cuticular proteins, that is, Ld-GRP1 through 3, have been identified in the Colorado potato beetle Leptinotarsa decemlineata , and the transcripts of Ld-GRP1 and Ld-GRP2 have been detected in the epidermal cell layer via in situ hybridization 17 .…”

Section: Resultsmentioning

confidence: 99%

Characterization of the transcriptomes and cuticular protein gene expression of alate adult, brachypterous neotenic and adultoid reproductives of Reticulitermes labralis

Xiao

et al. 2016

Sci Rep

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The separation of primary reproductive and secondary reproductive roles based on the differentiation of alate adults and neotenic reproductives is the most prominent characteristic of termites. To clarify the mechanism underlying this differentiation, we sequenced the transcriptomes of alate adults (ARs), brachypterous neotenics (BNs) and adultoid reproductives (ANs) from the last instar nymphs of Reticulitermes labralis. A total of 404,152,188 clean sequencing reads was obtained and 61,953 unigenes were assembled. Of the 54 identified cuticular protein (CP) genes of the reproductives, 22 were classified into the CPR family and 7 were classified into the CPG family. qRT-PCR analyses of the 6 CP genes revealed that the CP genes involved in exocuticle sclerotization were highly expressed in the ARs and RR-1 involved in soft endocuticle was highly expressed in the ARs and ANs. These results suggest that the alate adults might increase cuticular component deposition to adapt to new or changing environments and that the development of reproductive individuals into primary or secondary reproductives is controlled by the expression of cuticular protein genes involved in the hardening of the exocuticle. In addition, the AN caste is a transitional type between the BN and AR castes in the process of evolution.

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Section: Resultsmentioning

confidence: 99%

Characterization of the transcriptomes and cuticular protein gene expression of alate adult, brachypterous neotenic and adultoid reproductives of Reticulitermes labralis

Xiao

et al. 2016

Sci Rep

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“…These numbers, as well as those given for other insects, likely represent a minimum as most genomes have not gone through a rigorous manual annotation and rely on homology to other known CPs. Combining proteomics with genomics has been used to identify cuticular proteins for several insect species (Bae et al ., 2011; Carrasco et al ., 2011; Dittmer et al ., 2012; Fu et al ., 2011; He et al ., 2007). Similarly, genomics and transcriptomics have contributed valuable information on CP gene expression, discerning variations in the timing (pre- or post-molt), developmental stage (larval, pupal, adult), and relative expression levels of these genes (Cornman and Willis, 2009; Dittmer et al ., 2012; Futahashi et al ., 2008; Gallot et al ., 2010; Liang et al ., 2010; Okamoto et al ., 2008; Suetsugu et al ., 2013; Togawa et al ., 2007, 2008).…”

Section: Introductionmentioning

confidence: 99%

Annotation and expression analysis of cuticular proteins from the tobacco hornworm, Manduca sexta

Dittmer

Tetreau

Cao

et al. 2015

Insect Biochemistry and Molecular Biology

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The insect cuticle is a unique material that covers the exterior of the animal as well as lining the foregut, hindgut, and tracheae. It offers protection from predators and desiccation, defines body shape, and serves as an attachment site for internal organs and muscle. It has demonstrated remarkable variations in hardness, flexibility and elasticity, all the while being light weight, which allows for ease of movement and flight. It is composed primarily of chitin, proteins, catecholamines, and lipids. Proteomic analyses of cuticle from different life stages and species of insects has allowed for a more detailed examination of the protein content and how it relates to cuticle mechanical properties. It is now recognized that several groups of cuticular proteins exist and that they can be classified according to conserved amino acid sequence motifs. We have annotated the genome of the tobacco hornworm, Manduca sexta, for genes that encode putative cuticular proteins that belong to seven different groups: proteins with a Rebers and Riddiford motif (CPR), proteins analogous to peritrophins (CPAP), proteins with a tweedle motif (CPT), proteins with a 44 amino acid motif (CPF), proteins that are CPF-like (CPFL), proteins with an 18 amino acid motif (18 aa), and proteins with two to three copies of a C-X5-C motif (CPCFC). In total we annotated 248 genes, of which 207 belong to the CPR family, the most for any insect genome annotated to date. Additionally, we discovered new members of the CPAP family and determined that orthologous genes are present in other insects. We established orthology between the M. sexta and Bombyx mori genes and identified duplication events that occurred after separation of the two species. Finally, we utilized 52 RNAseq libraries to ascertain gene expression profiles that revealed commonalities and differences between different tissues and developmental stages.

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“…Working on proteomic characterization of butterfly wings [], we observed two spots identified as BBP and as this protein is showing highest similarity with mammalian apolipoprotein D [] and no mass spectrometric analyses have been published to the best of our knowledge, it was the aim of the study to present results from mass spectrometric analysis results of BBP from the wing of Hebomoia glaucippe (Fig. ) and their posttranslational modifications by a gel‐based mass spectrometrical technique.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometrical analysis of bilin‐binding protein from the wing of Hebomoia glaucippe (Linnaeus, 1758) (Lepidoptera: Pieridae)

et al. 2012

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Bilin-binding protein (BBP) is a member of the lipocalin superfamily and a pigment protein in Lepidoptera. It is binding to a series of lipidic compounds but its functions remain to be elucidated. Working on wing proteins in Hebomoia glaucippe, we observed this protein on gels and decided to characterize BBP. A gel-based mass spectrometrical method using two-dimensional gel electrophoresis followed by in-gel digestion of protein spots followed by nano-LC-ESI-MS/MS (ion trap, HCT) identification and characterization of proteins was applied. An antibody was generated against the protein and immunoblotting in the butterfly and mouse brain was carried out. Two spots were identified from the butterfly wing as BBP (P09464) with high sequence coverage. Nitrotyrosination (Y163; as aminotyrosine) was observed and nitration was verified using immunoblotting. Additional posttranslational modifications (PTMs) as hypusine, carboxylation, kynurenine, aminoadipic acid, were proposed. The presence of BBP-immunoreactive protein was also observed in mouse brain. The characterization of BBP showed high sequence similarity with mouse apolipoprotein D and the findings suggest a tentative function of BBP comparable to apolipoproteins. The role of the PTMs remains elusive but nitration, in analogy to nitration effects reported in literature, proposes a role for mechanoelastic proteins and protein-protein interactions.

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Mass spectrometrical analysis of cuticular proteins from the wing of Hebemoia glaucippe (Linnaeus, 1758) (Lepidoptera: Pieridae)

Cited by 5 publications

References 32 publications

Characterization of the transcriptomes and cuticular protein gene expression of alate adult, brachypterous neotenic and adultoid reproductives of Reticulitermes labralis

Characterization of the transcriptomes and cuticular protein gene expression of alate adult, brachypterous neotenic and adultoid reproductives of Reticulitermes labralis

Annotation and expression analysis of cuticular proteins from the tobacco hornworm, Manduca sexta

Mass spectrometrical analysis of bilin‐binding protein from the wing of Hebomoia glaucippe (Linnaeus, 1758) (Lepidoptera: Pieridae)

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