Mass spectrometry analysis of phosphotyrosine‐containing proteins

Li, Jiajia; Zhan, Xianquan

doi:10.1002/mas.21836

Cited by 4 publications

(2 citation statements)

References 157 publications

(309 reference statements)

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“…In the present review, we have outlined a brief description of the proteomics technologies applied to CPP and VPD studies. More extensive reviews on proteomics technologies have been published recently that provide additional details [27][28][29].…”

Section: Proteomics Methodsmentioning

confidence: 99%

Chronic Pelvic Pain, Vulvar Pain Disorders, and Proteomics Profiles: New Discoveries, New Hopes

Di Tucci,

Muzii

2023

Biomedicines

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Chronic pelvic pain (CPP) is generally defined as non-cyclic pain perceived in the pelvic area that has persisted from three to six months or longer and is unrelated to pregnancy. The etiology of CPP is complex, multifactorial, with heterogeneous presentation, and includes several diseases such as endometriosis, adenomyosis, and interstitial cystitis/bladder pain syndrome. It may also be associated with sexual dysfunction, musculoskeletal disorders, and comorbid psychiatric symptoms. Vulvar pain disorders (VPDs) are typically categorized separately from chronic pelvic pain; among all VPDs, vulvodynia is a chronic vulvar pain of unknown etiology, lasting at least 3 months and that might be associated with other potentially linked factors. Proteomics represents a useful approach to study the proteome profiles of clinical samples. In this review, we have considered a selection of articles that have analyzed the protein abundance and novel protein species from various biological samples, including eutopic/ectopic endometrium, urine, serum, follicular, peritoneal fluid, and cervical mucus, potentially involved in the pathogenesis and progression of CPP and VPDs. These findings could represent valuable targets for paving the way for the differential diagnosis and therapeutic management of CPP and VDPs, thereby optimizing both the prevention and treatment of these conditions.

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Section: Proteomics Methodsmentioning

confidence: 99%

Chronic Pelvic Pain, Vulvar Pain Disorders, and Proteomics Profiles: New Discoveries, New Hopes

Di Tucci,

Muzii

2023

Biomedicines

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“…One of the most common and powerful tools for studying the phosphoproteome is quantitative liquid chromatography-coupled mass spectrometry (LC-MS/MS). The power of LC-MS/MS for studying the phosphoproteome lies in its ability to accurately identify and quantify the exact sites of thousands of phosphorylation events on proteins in complex biological samples within hours [2][3][4][5][6][7][8]. LC-MS/MS is unrivaled in its speed, sensitivity, and throughput for phosphorylation site identification and quantification.…”

Section: Introductionmentioning

confidence: 99%

Phosphoproteomic Approaches for Identifying Phosphatase and Kinase Substrates

DeMarco

Hall

2023

Molecules

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Protein phosphorylation is a ubiquitous post-translational modification controlled by the opposing activities of protein kinases and phosphatases, which regulate diverse biological processes in all kingdoms of life. One of the key challenges to a complete understanding of phosphoregulatory networks is the unambiguous identification of kinase and phosphatase substrates. Liquid chromatography-coupled mass spectrometry (LC-MS/MS) and associated phosphoproteomic tools enable global surveys of phosphoproteome changes in response to signaling events or perturbation of phosphoregulatory network components. Despite the power of LC-MS/MS, it is still challenging to directly link kinases and phosphatases to specific substrate phosphorylation sites in many experiments. Here, we survey common LC-MS/MS-based phosphoproteomic workflows for identifying protein kinase and phosphatase substrates, noting key advantages and limitations of each. We conclude by discussing the value of inducible degradation technologies coupled with phosphoproteomics as a new approach that overcomes some limitations of current methods for substrate identification of kinases, phosphatases, and other regulatory enzymes.

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