Mass spectrometry applied to diagnosis, prognosis, and therapeutic targets identification for the novel coronavirus SARS-CoV-2: A review

Lima, Nerilson Marques; Fernandes, Bruno Luiz Mendes; Alves, Guilherme Figueira; Souza, Jéssica de; Siqueira, Marcelo Magno de; Nascimento, Maria Patrícia do; Moreira, Olívia B. O.; Sussulini, Alessandra; Oliveira, Marcone Augusto Leal de

doi:10.1016/j.aca.2021.339385

Cited by 11 publications

(6 citation statements)

References 87 publications

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“…With the improvement of complementary instrumentations in recent years, MS can be used for the detection and quantification of relevant biomolecules in the dynamics of organisms, including proteins, carbohydrates, nucleic acids, and lipids [ 98 ]. Now, MS has been used for the identification of SARS-CoV-2 and analysis of infection phases with high flux and high accuracy [ 99 ]. Compared with PCR and LFIA, the sampling of MS is none or less invasive.…”

Section: Sars-cov-2 Infection Mechanism and Diagnostic Methodsmentioning

confidence: 99%

Visual Detection of COVID-19 from Materials Aspect

Wang

Meng

et al. 2022

Adv. Fiber Mater.

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In the recent COVID-19 pandemic, World Health Organization emphasized that early detection is an effective strategy to reduce the spread of SARS-CoV-2 viruses. Several diagnostic methods, such as reverse transcription-polymerase chain reaction (RT-PCR) and lateral flow immunoassay (LFIA), have been applied based on the mechanism of specific recognition and binding of the probes to viruses or viral antigens. Although the remarkable progress, these methods still suffer from inadequate cellular materials or errors in the detection and sampling procedure of nasopharyngeal/oropharyngeal swab collection. Therefore, developing accurate, ultrafast, and visualized detection calls for more advanced materials and technology urgently to fight against the epidemic. In this review, we first summarize the current methodologies for SARS-CoV-2 diagnosis. Then, recent representative examples are introduced based on various output signals (e.g., colorimetric, fluorometric, electronic, acoustic). Finally, we discuss the limitations of the methods and provide our perspectives on priorities for future test development. Graphical Abstract

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Section: Sars-cov-2 Infection Mechanism and Diagnostic Methodsmentioning

confidence: 99%

Visual Detection of COVID-19 from Materials Aspect

Wang

Meng

et al. 2022

Adv. Fiber Mater.

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“…27 Mass spectrometry (MS) has also emerged as a powerful tool for analysis of proteases such as PLpro, affording accurate masses of cleavage peptides and intact proteins. Liquid chromatography mass spectrometry analysis of tryptic digests of PLpro (i.e., bottom-up proteomics) has shown utility in assays for detecting SARS-CoV-2 via identification of peptides from the virus in complex matrices such as blood and urine, 28 and this method has also localized covalently bound inhibitors to PLpro. 27,29 Crosslinking mass spectrometry revealed that interferon-induced ISG15 protein binds in a different region of PLpro compared to other ubiquitin and dimeric ubiquitin.…”

Section: Introductionmentioning

confidence: 99%

Native mass spectrometry reveals binding interactions of SARS-CoV-2 PLpro with inhibitors and ISG15

James,

Godula,

Perez

et al. 2024

Preprint

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Here we used native mass spectrometry (native MS) to probe a SARS-CoV protease, PLpro, which plays critical roles in coronavirus disease by affecting viral protein production and antagonizing host antiviral responses. Ultraviolet photodissociation (UVPD) and variable temperature electrospray ionization (vT ESI) were used to localize binding sites of PLpro inhibitors and revealed the stabilizing effects of inhibitors on protein tertiary structure. We compared PLpro from SARS-CoV-1 and SARS-CoV-2 in terms of inhibitor and ISG15 interactions to discern possible differences in protease function. A PLpro mutant lacking a single cysteine was used to localize inhibitor binding, and thermodynamic measurements revealed that inhibitor PR-619 stabilized the folded PLpro structure. These results will inform further development of PLpro as a therapeutic target against SARS-CoV-2 and other emerging coronaviruses.

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“…A hybrid mass spectrometer is the preferred analytical instrument in numerous fields for studying highly complex samples. [1][2][3][4][5][6] Hybrid instruments combine more than one type of mass analyzer into single mass spectrometer to achieve desirable performance in terms of tandem mass spectrometry, high mass resolving power, mass measurement accuracy, and fast data acquisition time by incorporating individual performance characteristics of each mass analyzer. [7][8][9] Over the past several years, various types of hybrid mass spectrometers have been developed and are now commercially available.…”

Section: Introductionmentioning

confidence: 99%

A planar quadrupole device for transmitting and trapping ions in high vacuum

Park,

Mohr,

Anderson

et al. 2023

Rapid Comm Mass Spectrometry

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RationaleHybrid mass spectrometers combine multiple mass analyzers to achieve optimal performance in terms of tandem mass spectrometry, high mass resolving power, and mass measurement accuracy for studying highly complex samples. As a result, the need for transport, trapping, and control of ion kinetic energies is critical for the successful integration of multiple mass analyzers and hybrid instrument operation. In addition, transportation of ion populations between two physically distinct locations can result in time‐of‐flight (TOF) discrimination against ions with widely disparate m/z values, compromising full mass spectral performance. In this work, we demonstrated a new ion guide, referred to as a planar quadrupole (PQ) ion guide, composed of two parallel printed circuit boards (PCB) that allow radiofrequency (RF) and direct current (DC) voltages to be combined to enable both axial transport and trapping of ion populations in the ultrahigh vacuum region of the mass spectrometer. As compared with a conventional multipole ion guide, the PQ ion guide showed comparable performance in ion m/z values, signal‐to‐noise, and intensity and effectively reduced mass discrimination caused by TOF effects.MethodsA PQ device was developed with two PCBs and simulated with SIMION 8.1. Electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry instrumentation were used for the testing of PQ performance.Results.In this work, we demonstrated a planar quadrupole (PQ) ion guide composed of two parallel PCB plates. The PQ enables both axial ion transport and trapping of ion populations throughout the ion transfer process from a LTQ to an ICR cell. As compared with a conventional multipole ion guide, the PQ showed comparable ion transmission efficiency and effectively reduced mass discrimination caused by TOF effects.ConclusionsThe PQ is a simple design that can be implemented for ion transmission and trapping on virtually any mass spectrometer.

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Mass spectrometry applied to diagnosis, prognosis, and therapeutic targets identification for the novel coronavirus SARS-CoV-2: A review

Cited by 11 publications

References 87 publications

Visual Detection of COVID-19 from Materials Aspect

Visual Detection of COVID-19 from Materials Aspect

Native mass spectrometry reveals binding interactions of SARS-CoV-2 PLpro with inhibitors and ISG15

A planar quadrupole device for transmitting and trapping ions in high vacuum

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