Mass Spectrometry-based Lipidomics and Its Application toBiomedical Research

Wu, Zhexue; Shon, Jong Cheol; Liu, Kwang-Hyeon

doi:10.15280/jlm.2014.4.1.17

Cited by 46 publications

(29 citation statements)

References 128 publications

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“…Recent studies demonstrate the feasibility of mass spectrometry-based lipidomics as a tool to identify and quantitate circulating bioactive lipids in biofluids of healthy and diseased patients [29]. Because bioactive lipids are emerging as signature molecules of development and progression of many diseases [17, 18, 29], in the near future lipidomics could become a standard clinical diagnostic tool in molecular medicine.…”

Section: Discussionmentioning

confidence: 99%

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Plasma cross-gestational sphingolipidomic analyses reveal potential first trimester biomarkers of preeclampsia

et al. 2017

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IntroductionPreeclampsia (PE) is a gestational disorder, manifested in the second half of pregnancy by maternal hypertension, proteinuria and generalized edema. PE is a major cause of maternal and fetal morbidity and mortality, accounting for nearly 40% of all premature births worldwide. Bioactive sphingolipids are emerging as key molecules involved in etiopathogenesis of PE, characterized by maternal angiogenic imbalance and symptoms of metabolic syndrome. The aim of this study was to compare the cross-gestational profile of circulating bioactive sphingolipids in maternal plasma from preeclamptic (PE) versus normotensive control (CTL) subjects with the goal of identifying sphingolipids as candidate first trimester biomarkers of PE for early prediction of the disease.MethodsA prospective cohort of patients was sampled at the first, second and third trimester of pregnancy for each patient (11–14, 22–24, and 32–36 weeks´ gestation). A retrospective stratified study design was used to quantify different classes of sphingolipids in maternal plasma. We used a reverse-phase high-performance liquid chromatography-tandem mass spectrometry (HPLC-ESI-MS/MS) approach for determining different sphingolipid molecular species (sphingosine-1-phosphate (S1P), dihydro-sphingosine-1-phosphate (DH-S1P), sphingomyelins (SM) and ceramides (Cer)) in cross-gestational samples of human plasma from PE (n = 7, 21 plasma samples across pregnancy) and CTL (n = 7, 21 plasma samples across pregnancy) patients.ResultsPlasma levels of angiogenic S1P did not change significantly in control and in preeclamptic patients´ group across gestation. DH-S1P was significantly decreased in second trimester plasma of PE patients in comparison to their first trimester, which could contribute to reduced endothelial barrier observed in PE. The major ceramide species (Cer 16:0 and Cer 24:0) tended to be up-regulated in plasma of control and PE subjects across gestation. The levels of a less abundant plasma ceramide species (Cer 14:0) were significantly lower in first trimester plasma of PE patients when compared with their gestational-matched control samples (p = 0.009). Major plasma sphingomyelin species (SM 16:0, SM 18:1 and SM 24:0) tended to be higher in control pregnancies across gestation. However, in PE patients, SM 16:0, SM 18:0 and SM 18:1 showed significant up-regulation across gestation, pointing to atherogenic properties of the sphingomyelins and particularly the potential contribution of SM 18:0 to the disease development. In addition, two major sphingomyelins, SM 16:0 and SM 18:0, were significantly lower in first trimester plasma of PE patients versus first trimester samples of respective controls (p = 0.007 and p = 0.002, respectively).ConclusionsCross-gestational analysis of maternal plasma of preeclamptic and normotensive women identifies differences in the biochemical profile of major sphingolipids (DH-S1P, sphingomyelins and ceramides) between these two groups. In addition, first trimester maternal plasma sphingolipids (Cer 14:0, SM 16:0 an...

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Section: Discussionmentioning

confidence: 99%

“…Because bioactive lipids are emerging as signature molecules of development and progression of many diseases [17, 18, 29], in the near future lipidomics could become a standard clinical diagnostic tool in molecular medicine.…”

Section: Discussionmentioning

confidence: 99%

Plasma cross-gestational sphingolipidomic analyses reveal potential first trimester biomarkers of preeclampsia

et al. 2017

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“…The knowledge about functional outcomes of affected metabolic composition of the brain is extensive in terms of neurotransmitters but scarce for the other aforementioned groups of metabolites. Remarkably, the role of brain lipid composition has been considered to influence the brain homeostasis [15][16][17]. However, it is not validated how the brain metabolites are affected by exercise.…”

Section: Introductionmentioning

confidence: 99%

Physical activity reduces anxiety and regulates brain fatty acid synthesis

et al. 2020

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Physical activity impacts brain functions, but the direct mechanisms of this effect are not fully recognized or understood. Among multidimensional changes induced by physical activity, brain fatty acids (FA) appear to play an important role; however, the knowledge in this area is particularly scarce. Here we performed global metabolomics profiling of the hippocampus and the frontal cortex (FC) in a model of voluntary running in mice. Examined brain structures responded differentially to physical activity. Specifically, the markers of the tricarboxylic acid (TCA) cycle were downregulated in the FC, whereas glycolysis was enhanced in the hippocampus. Physical activity stimulated production of myristic, palmitic and stearic FA; i.e., the primary end products of de novo lipogenesis in the brain, which was accompanied by increased expression of hippocampal fatty acid synthase (FASN), suggesting stimulation of lipid synthesis. The changes in the brain fatty acid profile were associated with reduced anxiety level in the running mice. Overall, the study examines exercise-related metabolic changes in the brain and links them to behavioral outcomes.

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“…In addition, the metabolism of lipid compounds is also strongly related to diabetes, liver cancer and breast cancer. 10 In 2003, the concept of lipidomics was put forward by scholars, which is a comprehensive description of the species and biological function of lipid molecules. 11,12 As the emerging and most important branch of metabolomics related to human health, lipidomic identication and quantication in living organisms have provided a new chance to better understand the functional role of various lipids in disease development.…”

Section: Introductionmentioning

confidence: 99%

Retracted Article: High performance liquid chromatography coupled with high resolution mass spectrometry-based characterization of lipidomic responses from rats with kidney injuries

Liu

et al. 2018

RSC Adv.

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a Metabolism of lipids is essential for the regulation of a variety of key cellular functions. Recent advances in high performance liquid chromatography coupled with high resolution mass spectrometry have expanded our knowledge of lipid metabolism in diseases. Currently, sepsis is one of the most important public health problems all over the world, which is a serious systemic inflammatory syndrome leading to infection by various agents or trauma and subsequently to a multiple organ dysfunction response. However, little is known about the lipids affected by sepsis and their roles in kidney injuries. In this study, we present targeted and non-targeted lipidomics strategies to discover the lipid metabolism variation in serum in rats with sepsis-induced kidney injuries. Liquid chromatography (LC) coupled with mass spectrometry (MS) and multivariate data analysis were used to obtain the global lipid metabolic profiles. In addition, biochemical parameters and histopathological examination results for the kidney were analyzed to support the pathological changes during sepsis-induced kidney injury. The identification of ten proposed lipids and five relevant pathways will promote a better understanding of lipid profile alterations in kidney injury. The results suggested that lipid metabolism in sepsis-induced kidney injury had changed significantly and contribute by offering potential targets for clinical diagnosis and therapy in the future, which would be worth further studies to broaden the applications of high performance liquid chromatography coupled with high resolution mass spectrometry in the study of lipid metabolism.

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Mass Spectrometry-based Lipidomics and Its Application toBiomedical Research

Cited by 46 publications

References 128 publications

Plasma cross-gestational sphingolipidomic analyses reveal potential first trimester biomarkers of preeclampsia

Plasma cross-gestational sphingolipidomic analyses reveal potential first trimester biomarkers of preeclampsia

Physical activity reduces anxiety and regulates brain fatty acid synthesis

Retracted Article: High performance liquid chromatography coupled with high resolution mass spectrometry-based characterization of lipidomic responses from rats with kidney injuries

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