Mass spectrometry–based lipidomics of oral squamous cell carcinoma tissue reveals aberrant cholesterol and glycerophospholipid metabolism — A Pilot study

Dickinson, Amy; Saraswat, Mayank; Joenväära, Sakari; Agarwal, Rahul; Jyllikoski, Daniel; Wilkman, Tommy; Mäkitie, Antti; Silén, Suvi

doi:10.1016/j.tranon.2020.100807

Cited by 31 publications

(23 citation statements)

References 46 publications

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“…The KEGG pathway analysis revealed GPL metabolism as the most enriched pathway. In OSCC, GPL metabolism is the most abundant form of lipid metabolism 18 . These data indicated the molecular mechanism underlying lipid metabolism in HNSCC.…”

Section: Discussionmentioning

confidence: 70%

“…FADS1 has emerged as mediator of lipid metabolism gene and drives laryngeal squamous cell carcinoma progression by activating AKT/mTOR signaling pathway 17 . In oral squamous cell carcinoma (OSCC), the Kennedy signaling pathway is upregulated by cholesterol and glycerophospholipid (GPL) metabolic changes 18 . While lipid metabolism is vital for HNC, its role is not completely understood in HNSCC.…”

Section: Discussionmentioning

confidence: 99%

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Prognostic value of lipid metabolism‐related genes in head and neck squamous cell carcinoma

Xiong

Feng

et al. 2020

Immunity Inflam & Disease

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Background Altered lipid metabolism is involved in the development of many tumors. However, the role of dissimilar lipid metabolism in head and neck squamous cell carcinoma (HNSCC) is not fully established. Aims Here, we sought to determine the prognostic value of lipid metabolism‐related genes in HNSCC. Methods RNA‐seq data and clinical features of 545 HNSCC cases were obtained from The Cancer Genome Atlas database. A regulatory network of transcription factors‐lipid metabolism genes and a risk prognostic model of lipid metabolism‐related genes was developed using bioinformatics and Cox regression modeling. We used tumor immune estimation resource to analyze immune cell infiltration in patients with HNSCC based on the prognostic index (PI) of lipid metabolism‐related genes. Results A total of 136 differentially expressed lipid metabolism genes were identified. Of these, 23 are related to prognosis. In addition to predicting HNSCC prognosis, 11 lipid metabolism‐related genes (ARSI, CYP27B1, CYP2D6, DGKG, DHCR7, LPIN1, PHYH, PIP5K1B, PLA2G2D, RDH16, and TRIB3) also affect HNSCC clinical features (stage, gender, and pathological stage). The PI of lipid metabolism‐related genes embodied the state of HNSCC tumor immune microenvironment.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Prognostic value of lipid metabolism‐related genes in head and neck squamous cell carcinoma

Xiong

Feng

et al. 2020

Immunity Inflam & Disease

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“…Moreover, the occurrence and development of these two cancers were inseparable from the glycerophospholipid metabolic pathway. In oral tongue squamous-cell cancer, Dickinson et al performed metabolic analysis between the tumor tissue and healthy oral tongue mucosa samples from 10 oral squamous-cell carcinoma (OSCC) patients, where the glycerophospholipid metabolism was aberrant [58]. Preoperative plasma samples were collected from 50 patients with OSCC and 50 normal patients by lipid profiling; all glycerophospholipids were decreased [59].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models

Miao

Wang

et al. 2022

Biomedicines

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In recent years, the emerging technology of cold atmospheric pressure plasma (CAP) has grown rapidly along with the many medical applications of cold plasma (e.g., cancer, skin disease, tissue repair, etc.). Plasma-activated liquids (e.g., culture media, water, or normal saline, previously exposed to plasma) are being studied as cancer treatments, and due to their advantages, many researchers prefer plasma-activated liquids as an alternative to CAP in the treatment of cancer. In this study, we showed that plasma-activated-saline (PAS) treatment significantly inhibited tumor growth, as compared with saline, in melanoma, and a low-pH environment had little effect on tumor growth in vivo. In addition, based on an ultra-high-performance liquid tandem chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) analysis of tumor cell metabolism, the glycerophospholipid metabolic pathway was the most susceptible metabolic pathway to PAS treatment in melanoma in vitro and in vivo. Furthermore, PAS also inhibited cell proliferation in vivo in oral tongue squamous-cell cancer and non-small-cell lung cancer. There were few toxic side effects in the three animal models, and the treatment was deemed safe to use. In the future, plasma-activated liquids may serve as a potential therapeutic approach in the treatment of cancer.

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“…Accordingly, cholesterol accumulation has been reported in oral squamous cell carcinoma, clear-cell renal carcinoma, prostate cancer, and breast cancer. 7–10 …”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, cholesterol accumulation has been reported in oral squamous cell carcinoma, clear-cell renal carcinoma, prostate cancer, and breast cancer. [7][8][9][10] Squalene epoxidase (SQLE) is a monooxygenase that catalyzes the conversion of squalene to 2,3(S)oxidosqualene. 11 SQLE is the second rate-limiting enzyme in the cholesterol synthesis pathway, and its inhibition effectively reduces cholesterol synthesis.…”

Section: Introductionmentioning

confidence: 99%

High SQLE Expression and Gene Amplification Correlates with Poor Prognosis in Head and Neck Squamous Cell Carcinoma

Liu¹,

Fang²,

Liu³

2021

CMAR

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Objective Squalene epoxidase (SQLE) is considered a metabolic oncogene, but its biological function and prognostic value in head and neck squamous cell carcinoma (HNSCC) remain unclear. We aimed to evaluate the role of SQLE in the occurrence and development of HNSCC through bioinformatics analysis, and validation experiments. Methods Transcriptomic, genomic, and clinical data from The Cancer Genome Atlas were used for pan-cancer analysis. SQLE expression in HNSCC was evaluated using Gene Expression Omnibus datasets and immunohistochemistry. The biological significance of SQLE in the tumor microenvironment (TME) of HNSCC was determined using TISCH, HuRI, LinkedOmics, and TIMER 2.0. The prognostic value of SQLE in HNSCC was analyzed using univariate Cox regression and Kaplan–Meier survival curves. Effect of SQLE on the Cal27 HNSCC cell line was evaluated using cell counting kit 8, wound healing, and EdU assays. Results SQLE was overexpressed and amplified in various cancers, including HNSCC. High SQLE expression promoted cell proliferation, associated with T stage in HNSCC patients. Copy number amplification and DNA demethylation contributed to high SQLE expression in HNSCC, which was associated with poor prognosis. SQLE was related to HNSCC TME, and its mRNA expression/copy number alterations were negatively correlated with the infiltration of CD8+ T cells, follicular helper T cells, and regulatory T cell infiltration and mast cell activation and positively correlated with the infiltration of M0 macrophages and resting mast cells in HNSCC. Conclusion SQLE was identified as a prognostic biomarker and a potential pharmaceutical target for HNSCC.

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Mass spectrometry–based lipidomics of oral squamous cell carcinoma tissue reveals aberrant cholesterol and glycerophospholipid metabolism — A Pilot study

Cited by 31 publications

References 46 publications

Prognostic value of lipid metabolism‐related genes in head and neck squamous cell carcinoma

Prognostic value of lipid metabolism‐related genes in head and neck squamous cell carcinoma

In Vivo Metabolic Analysis of the Anticancer Effects of Plasma-Activated Saline in Three Tumor Animal Models

High SQLE Expression and Gene Amplification Correlates with Poor Prognosis in Head and Neck Squamous Cell Carcinoma

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