Mass spectrometry-based metabolomics investigation on two different indica rice grains (Oryza sativa L.) under cadmium stress

Zeng, Ting; Fang, Baohua; Huang, Fenglin; Li, Dai; Zhi, Tian; Tian, Jinglin; Cao, Guodong; Meng, Xuanlin; Liu, Yuanchen; Lei, Bo; Lu, Minghua; Cai, Zongwei

doi:10.1016/j.foodchem.2020.128472

Cited by 42 publications

(21 citation statements)

References 20 publications

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“…D-Mannitol, involved in fructose and mannose metabolism, was down-regulated in both two varieties. It could be a potential scavenger of ROS in rice due to its osmotic regulation function [ 27 ]. It has been defined as a potential biomarker in high-cadmium tolerant rice [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

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Metabolomic Analysis Reveals Insights into Deterioration of Rice Quality during Storage

Wang

Zhang

Zhao

et al. 2022

Foods

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To determine the changes in the quality of rice during storage, this study investigated the comprehensive metabolomic profiles of Nanjing 9108 (typical japonica rice) and Jianzhen 2 (typical indica rice) varieties in China, using metabolomics. A total of 13 categories of 593 metabolites including lipids (134 species), phenolic acids (78 species), flavonoids (70 species), alkaloids (67 species), organic acids (64 species), amino acids and derivatives (64 species), saccharides and alcohols (44 species), nucleotides and derivatives (37 species), vitamins (14 species), lignans and coumarins (9 species), tannins (2 species), terpenoids (2 species), and others (8 species) were identified in both varieties. The result showed significant changes in 204 metabolites in Nanjing 9108, while only 26 were altered in Jianzhen 2 during storage. These metabolites involved 46 metabolic pathways. The TCA cycle, linoleic, and α-linolenic acid metabolic pathways were unique in Nanjing 9108. Finally, the results of quantitative mass spectrometry of 11 metabolites provided insight into biomarkers associated with quality deterioration of rice. This study provides insights into the mechanism of deterioration in the quality of rice during storage.

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Section: Discussionmentioning

confidence: 99%

“…d -trehalose stabilizes proteins and membranes and plays an equivalent role to osmotic cells. The down-regulation of d -trehalose indicates deterioration in the regulatory function of rice [ 27 ]. The content of D-trehalose in NJS also decreased ( Figure 8 ), which demonstrated a stronger respiratory effect during storage.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Analysis Reveals Insights into Deterioration of Rice Quality during Storage

Wang

Zhang

Zhao

et al. 2022

Foods

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“…Most carbohydrates and amino acids are down-regulated as Cd increases, especially L-cysteine, and inositol is up-regulated as Cd increases to prevent Cd toxicity ( Jiang et al., 2020 ). Simultaneously, 13-(S) -hydroperoxy-9(Z), 11(E), 15(Z) -octadecatrienoic acid was activated as an organic acid, which was related to the metabolism of α-linolenic acid and the production of jasmonic acid ( Zeng et al., 2021 ). The 12 significantly DAMs of Amaranthus hypochondriacus under Cd stress were highly linearly correlated with phytochelatins (PCs), which involved in the pathways of valine (Val), leucine (Leu) and isoleucine (Ile) biosynthesis, alanine (Ala), aspartic acid (Asp) and glutamate (Glu) metabolism, and arginine (Arg) and proline (Pro) metabolism ( Xie et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolome and transcriptome association analysis revealed key factors involved in melatonin mediated cadmium-stress tolerance in cotton

Yan²,

Li³

et al. 2022

Front. Plant Sci.

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Cadmium (Cd), a non-essential element for plant, is a ubiquitous and highly toxic heavy metal, seriously endangering agricultural production and human health. As a nonedible economic crop, cotton (Gossypium hirsutum L.) has great potential in remediation of Cd contaminated soil, but its underlying mechanism is still unknown. Melatonin (MT), as a plant growth regulator, is involved in alleviating Cd toxicity in some plants, but the molecular mechanisms of MT-mediated Cd detoxification in cotton are largely unknown. This study investigated the possible molecular mechanisms of the MT-mediated Cd detoxification in cotton seedlings by comparative transcriptomic and metabolomic analyses. The results showed that the cotton seedlings were dwarfed and the leaves were wilted and yellow under Cd stress. The application of 50 µmol L−1 MT significantly increased the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content under Cd stress, but 100 µmol L−1 MT significantly decreased SOD activity, while increased ascorbate peroxidase (APX) activity significantly. The addition of 100 μmol L−1 MT significantly increased Cd concentration in the shoots and roots under Cd stress. RNA-seq analysis showed that 5573, 7105, 7253, 25, 198, 9 up-regulated and 6644, 7192, 7404, 9, 59, 0 down-regulated differentially expressed genes (DEGs) were identified in the comparisons of CK vs T1, CK vs T2, CK vs T3, T1 vs T2, T1 vs T3 and T2 vs T3, respectively. It was revealed that MT promoted the expression of certain related genes under Cd stress, and the effect of 100 µmol L−1 MT was better. Moreover, UPLC-MS/MS widely targeted metabolites analyses showed that 195, 150, 150, 12, 24, 59 up-regulated and 16, 11, 23, 38, 127, 66 down-regulated differentially accumulated metabolites (DAMs) were changed in the CK vs T1, CK vs T2, CK vs T3, T1 vs T2, T1 vs T3 and T2 vs T3, respectively. It was revealed that MT induced the synthesis of alkaloids and flavonoids, and inhibited or reduced the synthesis of lipids, amino acids and their derivatives. The comprehensive analyses of transcriptomic and metabolic data showed that 33 DEGs and 4 DAMs, 46 DEGs and 16 DAMs, and 1 DEGs and 1 DAMs were dominantly involved in the pathways of valine, leucine and isoleucine degradation, ABC transporter, alpha-linolenic acid metabolism, respectively. It was revealed that there were three major mechanisms involved in MT-mediated Cd detoxification in cotton, including the enhancement of antioxidant capacity regulated by APX, flavonoids and alkaloids; accumulation of secondary metabolites related to Cd chelation, such as amino acids and derivatives; and regulation of cadmium ion transportation, such as ABC transporter activation. In conclusion, this study provides new insights into the MT-mediated Cd stress response.

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“…Elevated CO 2 can regulate the generation of secondary metabolites in plants, increasing the contents of phenolic compounds, which is related to strong antioxidant capacity of plants (Akula et al 2011; Li et al 2018); When plants are stressed by heavy metals, the metabolites and metabolic pathways in plants will change accordingly, so as to achieve the purpose of self-protection. Zeng (2021) found that most of carbohydrates and amino acids of rice grain decreased under Cd stresses, energy metabolism was affected, content of inositol increased (has inhibitory effect on cadmium toxicity), 13 (S)-HPOT (associated with α-linolenic acid metabolism and jasmonic acid metabolism), 9, 10-DHOME (associated with linolenic acid metabolism), citric acid, hexadecanoic acid and organic acids contents increased.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics and Metabolomics Analysis Reveal the Molecular Responses of Rice to Cd Stress And/Or Elevated Co2 Concentration

Wang

Cui

et al. 2022

SSRN Journal

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Mass spectrometry-based metabolomics investigation on two different indica rice grains (Oryza sativa L.) under cadmium stress

Cited by 42 publications

References 20 publications

Metabolomic Analysis Reveals Insights into Deterioration of Rice Quality during Storage

Metabolomic Analysis Reveals Insights into Deterioration of Rice Quality during Storage

Metabolome and transcriptome association analysis revealed key factors involved in melatonin mediated cadmium-stress tolerance in cotton

Transcriptomics and Metabolomics Analysis Reveal the Molecular Responses of Rice to Cd Stress And/Or Elevated Co2 Concentration

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