Mass spectrometry-based proteomic approaches to study pathogenic bacteria-host interactions

Abstract

Elucidation of molecular mechanisms underlying host-pathogen interactions is important for control and treatment of infectious diseases worldwide. Within the last decade, mass spectrometry (MS)-based proteomics has become a powerful and effective approach to better understand complex and dynamic host-pathogen interactions at the protein level. Herein we will review the recent progress in proteomic analyses towards bacterial infection of their mammalian host with a particular focus on enteric pathogens. Large-scale studies of dynamic proteomic alterations during infection will be discussed from the perspective of both pathogenic bacteria and host cells.

Quantitative proteomic analysis of host epithelial cells infected by Salmonella enterica serovar Typhimurium

“…The recent progresses of high‐throughput technologies provide the possibilities to dissect host–pathogen interactions on an unbiased, global scale . Many groups have contributed to this aspect of Salmonella interactions with the host by using different “omics” approaches .…”

Editorial: Proteomics of Microbial Human Pathogens

“…During the last two decades, powerful high throughput research platforms—namely genomics, transcriptomics, and proteomics—have contributed significantly to our understanding of the life styles of pathogenic microbes, including important aspects of host interactions and infection (Raskin et al, 2006; Merhej et al, 2013; Yang et al, 2015). Of all the omics platforms, proteomics is perhaps the research platform that has undergone the greatest transformation, including a structural transformation, as it evolved from initial two-dimensional gel electrophoresis (2-DE gel) based workflows to sophisticated shotgun proteomics analysis, capable of generating comprehensive data with a coverage of individual proteomes that begins to approach that of corresponding genomics/transcriptomics datasets now.…”

Microbial Proteomics and Their Importance in Medical Microbiology

“…Also, the manipulation of the host ubiquitin pathways by viruses and bacterial effector proteins is a recurrent finding. By controlling protein degradation and cell signaling, ubiquitination is a critical regulator of various cellular processes such as inflammatory responses, vesicular trafficking, and cell cycle, altogether making it an ideal target to hijack for bacterial and viral pathogenicity (Martinez-Martin, 2017;Yang et al, 2015). These infection-involved proteins inevitably contribute to the elucidation of the path physiology of pathogen-host interactions.…”