Mass spectrometry characterization of endophytic bacterium <i>Curtobacterium</i> sp. strain ER1/6 isolated from <i>Citrus sinensis</i>

Araújo, Francisca Diana da Silva; Santos, Daiene Souza; Pagotto, Carolina Clepf; Araújo, Welington Luiz; Eberlin, Marcos N.

doi:10.1002/jms.4042

Cited by 6 publications

(6 citation statements)

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“…Lipid profiles have successfully been used to speciate bacteria using mass spectrometry − and even to detect strain level differences in antibiotic resistant bacteria . Typically, a mass spectrum can provide a metabolomic and lipidomic profile while MS/MS and collision-induced dissociation (CID) experiments provide structural information on specific features of interest. , Related methods involve the use of ion mobility coupled with MS, ,− rapid evaporative ionization mass spectrometry (REIMS), , matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), , direct analysis in real time mass spectrometry (DART-MS), , direct infusion mass spectrometry (DI-MS), and liquid chromatography coupled with mass spectrometry (LC-MS). , Many of these experiments are targeted at specific biomarkers, thus being limited to specific biological targets. Untargeted methods do not have the same limitations; however, they are time-consuming as many individual MS/MS spectra are required for each sample.…”

Section: Introductionmentioning

confidence: 99%

“…25 Typically, a mass spectrum can provide a metabolomic and lipidomic profile while MS/MS and collision-induced dissociation (CID) experiments provide structural information on specific features of interest. 24,26 Related methods involve the use of ion mobility coupled with MS, 24,26−28 rapid evaporative ionization mass spectrometry (REIMS), 29,30 matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), 31,32 direct analysis in real time mass spectrometry (DART-MS), 33,34 etry (DI-MS), 35 and liquid chromatography coupled with mass spectrometry (LC-MS). 36,37 Many of these experiments are targeted at specific biomarkers, thus being limited to specific biological targets.…”

Section: ■ Introductionmentioning

confidence: 99%

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Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

et al. 2022

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Lipidomic and metabolomic profiles of sporulated and vegetative Bacillus subtilis and Bacillus thuringiensis from irradiated lysates were recorded using a quadrupole ion trap mass spectrometer modified to perform two-dimensional tandem mass spectrometry (2D MS/MS). The 2D MS/MS data domains, acquired using a 1.2 s scan of negative ions generated by nanoelectrospray ionization of microwave irradiated spores, showed the presence of dipicolinic acid (DPA) as well as various lipids. Aside from microwave radiation to extract DPA and lipids from spores, sample preparation was minimal. Characteristic lipid and metabolic profiles were observed using 107108 cells of the two Bacillus species. Major features of the lipid profiles observed for the vegetative states included sets of phosphatidylglycerol (PG) lipids. Product ion spectra were extracted from the 2D MS/MS data, and they provided structural information on the fatty acid components of the PG lipids. The study demonstrates the flexibility, speed, and informative power of metabolomic and lipidomic fingerprinting for identifying the presence of spore-forming biological agents using 2D MS/MS as a rapid profiling screening method.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

et al. 2022

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“…Desorption electrospray ionization (DESI) impinges a steam of highly charged solvent droplets from an ESI source onto a solid sample: the scattered species containing the analyte are then captured by the mass spectrometry interface [ 32 , 58 ]. Samples analyzed by DESI are held at atmospheric pressure, whereas MALDI, SIMS, and LDI/LDPI are operated typically under a vacuum [ 13 ].…”

Section: Msi Ionization Techniques To Investigate Plant–microbe Inter...mentioning

confidence: 99%

Mass Spectral Imaging to Map Plant–Microbe Interactions

Parker

Hanley

2023

Microorganisms

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Plant–microbe interactions are of rising interest in plant sustainability, biomass production, plant biology, and systems biology. These interactions have been a challenge to detect until recent advancements in mass spectrometry imaging. Plants and microbes interact in four main regions within the plant, the rhizosphere, endosphere, phyllosphere, and spermosphere. This mini review covers the challenges within investigations of plant and microbe interactions. We highlight the importance of sample preparation and comparisons among time-of-flight secondary ion mass spectroscopy (ToF-SIMS), matrix-assisted laser desorption/ionization (MALDI), laser desorption ionization (LDI/LDPI), and desorption electrospray ionization (DESI) techniques used for the analysis of these interactions. Using mass spectral imaging (MSI) to study plants and microbes offers advantages in understanding microbe and host interactions at the molecular level with single-cell and community communication information. More research utilizing MSI has emerged in the past several years. We first introduce the principles of major MSI techniques that have been employed in the research of microorganisms. An overview of proper sample preparation methods is offered as a prerequisite for successful MSI analysis. Traditionally, dried or cryogenically prepared, frozen samples have been used; however, they do not provide a true representation of the bacterial biofilms compared to living cell analysis and chemical imaging. New developments such as microfluidic devices that can be used under a vacuum are highly desirable for the application of MSI techniques, such as ToF-SIMS, because they have a subcellular spatial resolution to map and image plant and microbe interactions, including the potential to elucidate metabolic pathways and cell-to-cell interactions. Promising results due to recent MSI advancements in the past five years are selected and highlighted. The latest developments utilizing machine learning are captured as an important outlook for maximal output using MSI to study microorganisms.

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“…Based on sequencing, the G + C content of the type strain is 72.2%. The endophytic bacterium C. uspiensis ER1/6 T reduces symptoms caused by Xylella fastidiosa in Catharanthus roseus (Lacava et al 2007) and in vitro (Lacava et al 2004), and produces, based on ESI-MS/MS fragmentation pro le, phospholipids including the classes of glycerophosphocholine, glycerophosphoglycerol, and glycerophosphoinositol as well as several fatty acids (Araújo et al 2018). Therefore, we screened the genome sequence for the presence of genes encoding for the biosynthesis of phospholipids, antibiotics, and other metabolites contributing to plant disease suppression.…”

Section: Genome and Secondary Metabolic Gene Clustersmentioning

confidence: 99%

Curtobacterium uspiensis sp. nov., an endophytic bacterium isolated from Citrus sinensis (sweet orange) in Brazil

Araújo

Belmonte

Dourado

et al. 2023

Preprint

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Endophytic bacteria were isolated from Citrus plants and based on the similarity of 16S rRNA gene sequence, 20 isolates were included in the genus Curtobacterium in the family Microbacteriaceae, class Actinobacteria. Amplified Fragment Length Polymorphism (AFLP) analysis indicated that these strains formed four clusters with low variability that were separated from Curtobacterium flaccumfaciens. The isolates were Gram-positive, non-motile, non-spore forming, pale-yellow to orange-pink colonies. Analysis of eleven isolates showed that the major fatty acids of these strains were anteiso-C15:0, anteiso-C17:0 and iso-C16:0 and MK-9 and MK-8 as the major isoprenoid quinone, supporting the affiliation into Curtobacterium genus. The similarity of 16S rRNA gene between these isolates ranged from 99.9 to 100%, suggesting that this endophytic population present a low variability and similarity to species with validity published names within this genus, forming a distinct group in the phylogenetic tree. The DNA–DNA relatedness values to closest species were less than 48% for ER1/6T, suggesting that this strain does not belong to already described species. Analysis of the ER1/6T genome detected genes predicted to be involved in secondary metabolites synthesis, such as siderophore Desferrioxamine-like, bacteriocin Lactococcin 972-like, and terpene C50 carotenoid-like. Based on genome sequencing, the G + C content of the strain ER1/6T was 72.2%. Therefore, the polyphasic taxonomic characterization demonstrated that the strains ER1/6T, ER1.4/2, SR4/1 and SR4/8 dominant in the citrus tissues, represent a new species of the genus Curtobacterium, for which the name Curtobacterium uspiensis sp. nov. is proposed, with strain ER1/6T = CBMAI 2131 as the type strain.

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Mass spectrometry characterization of endophytic bacterium Curtobacterium sp. strain ER1/6 isolated from Citrus sinensis

Cited by 6 publications

References 39 publications

Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

Metabolomic and Lipidomic Profiling of Bacillus Using Two-Dimensional Tandem Mass Spectrometry

Mass Spectral Imaging to Map Plant–Microbe Interactions

Curtobacterium uspiensis sp. nov., an endophytic bacterium isolated from Citrus sinensis (sweet orange) in Brazil

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