Mass spectrometry imaging: a new vision in differentiating<i>Schistosoma mansoni</i>strains

Ferreira, Mônica Siqueira; Oliveira, Diogo Noin de; Oliveira, Rosimeire Nunes de; Allegretti, Silmara Marques; Vercesi, Anı́bal E.; Catharino, Rodrigo Ramos

doi:10.1002/jms.3308

Cited by 30 publications

(30 citation statements)

References 35 publications

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“…For example, the granuloma formation occurs by the reaction of host immune response mediated by parasite glycoconjugates; therefore, the greater abundance of glucosylceramides (GlcCer) in one strain compared to another, the higher pathogenicity [32,34]. The same study using high-resolution mass spectrometry (HRMS) showed that phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylethanolamine (PE), triacylglycerol (TG), and diacylglycerol (DG) are involved in the differentiation of S. mansoni strains [34]. In respect to maturation, immature and mature eggs present also been differentiated using Matrix-Assisted Laser Desorption/ Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS).…”

Section: Mature and Immature Eggsmentioning

confidence: 99%

Mass Spectrometry and Metabolomics—New Approaches for Helminth Biochemical Studies

Lima¹,

Esteves²,

Oliveira³

et al. 2017

Human Helminthiasis

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Metabolomics, the study of the endogenously synthesized small molecules repertoire (nonproteinaceous), is of great relevance for establishing a wide view of cell physiology at specific moments, linking metabolic profiles to phenotypes and genotypes. To better understand biological systems, such as helminths life cycle, helminthic infection, and host-parasite interaction, metabolomics studies are crucial. For that, mass spectrometrybased metabolomics is the most popular strategy. Nontargeted metabolomics allows researchers to profile entire metabolomes present in cells, tissues, biofluids, or even samples as complex as stools. Through different mass spectrometric techniques, it is possible to unveil chemical markers for helminths, such as Schistosoma mansoni (a trematode) and Ascaris lumbricoides (a nematode), in addition to study mechanisms of action for different drugs, which targets parasites. Therefore, mass spectrometry allows designing biochemical pathways that may clarify the processes of parasite life cycle, helminthic infection, and host-parasite interaction, providing targets to further interference for parasite control or even infection treatment.

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Section: Mature and Immature Eggsmentioning

confidence: 99%

Mass Spectrometry and Metabolomics—New Approaches for Helminth Biochemical Studies

Lima¹,

Esteves²,

Oliveira³

et al. 2017

Human Helminthiasis

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“…Despite the ongoing belief that schistosomes cannot utilize fatty acid (or β‐) oxidation to support OXPHOS (Barrett, ; Ferreira et al ., 2014a,b), the schistosome genome nevertheless encodes the enzymes of the β‐oxidation pathway, through which fatty acids are catabolized into the TCA cycle (Berriman et al ., ), and mitochondrial oxygen consumption in schistosomes can be inhibited by etomoxir, a drug that blocks the transfer of activated fatty acids into mitochondria for subsequent oxidation (Huang et al ., ). Moreover, genetic loss of function of Acyl CoA synthase and Acyl CoA dehydrogenase, key enzymes in the β‐oxidation pathway, also results in diminished mitochondrial oxygen consumption.…”

Section: Fatty Acid Oxidation Is Required For Egg Productionmentioning

confidence: 99%

The metabolic control of schistosome egg production

Pearce

Huang

2015

Cell Microbiol

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Schistosomiasis is a Neglected Tropical Disease caused by infection with trematode parasites of the genus Schistosoma. Despite ongoing treatment programs, the prevalence of schistosomiasis has failed to decline and the disease remains a cause of severe morbidity in millions of people. Understanding the biology of egg production by schistosomes is critical since eggs allow transmission of the infection, and when trapped in host tissues induce the immune responses that are responsible for the pathologic changes that underlie disease development. Unusually among trematodes, adult schistosomes exhibit sexual dimorphism and display a fascinating codependency in that the female is dependent on the male to grow and sexually mature. Thus virgin females are developmentally stunted compared to females from mixed-sex infections and are unable to lay eggs. Moreover, fecund female schistosomes rapidly lose the ability to produce eggs when placed in tissue culture. Here we discuss the metabolic regulation of egg production in schistosomes, and in particular the critical role played by fatty acid oxidation in this process.

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“…With respect to investigation of the molecular content of the tissue within its morphological context, it was the technique of mass spectrometry imaging (MSI) that made it possible [24]. In parasitology, one study examined the chemical markers of the surface of S. mansoni by MSI to distinguish between the sexes and the strains [25], while another study dealt with the same organism and investigated the composition of internal organs by histological sections [26]. Because of the technical limits of MSI, in both cases, the focus was on the relatively small molecules of triacylglycerols and phosphatidylcholines, not on proteins.…”

Section: Introductionmentioning

confidence: 99%

Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes ofEudiplozoon nipponicum(Monogenea, Polyopisthocotylea)

Roudnický

Potěšil²,

Zdráhal³

et al. 2020

Preprint

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23Eudiplozoon nipponicum (Goto, 1891) is a hematophagous monogenean ectoparasite 24 which inhabits the gills of the common carp (Cyprinus carpio). Heavy infestation can lead to 25 anemia and in conjunction with secondary bacterial infections cause poor health and eventual 26 death of the host. 27 This study is based on an innovative approach to protein localization which has never 28 been used in parasitology before. Using laser capture microdissection, we dissected particular 29 areas of the parasite body without contaminating the samples by surrounding tissue, and in 30 combination with analysis by mass spectrometry obtained tissue-specific proteomes of 31 tegument, intestine, and parenchyma of our model organism, E. nipponicum. We successfully 32 verified the presence of certain functional proteins (e.g. cathepsin L) in tissues where their 33 presence was expected (intestine) and confirmed that there were no traces of these proteins in 34 other tissues (tegument and parenchyma). Additionally, we identified a total of 2,059 proteins, 35 including 72 peptidases and 33 peptidase inhibitors. As expected, the greatest variety was found 36 in the intestine and the lowest variety in the parenchyma. 37Our results are significant on two levels. Firstly, we demonstrated how one can localize 38 all proteins in one analysis and without using laboratory animals (antibodies for 39 immunolocalization of single proteins). Secondly, this study offers the first complex proteomic 40 data on not only the E. nipponicum but within the whole class of Monogenea, which was from 41 this point of view until recently neglected. 42 3 43 Introduction 44 Laser-capture microdissection (LCM) was developed as a powerful and reliable tool to 45 overcome the heterogeneity of the specimen [1]. It enables isolation of specific tissues, 46 individual cells, or even individual organelles from complex samples based on cell morphology 47 [2,3]. The method was first described in the second half of the 20 th century and since then further 48 developed and modified [2,4-7]. Nowadays, LCM combines laser excision with high-resolution 49 microscopic control, which makes it possible to precisely capture for instance individual cells 50 or even only nucleus while keeping track of the location and morphology of the source tissue 51 [2,3,8]. 52 LCM technology has been used in a wide variety of applications with focus on genomic, 53 transcriptomic, and even proteomic analyses, such as dissection of polar bodies from oocytes 54 for pre-fertilization genetic diagnosis [9], transcriptome-wide analysis of blood vessels from 55 human skin and wound-edge tissue [10], proteomic profiling of dentoalveolar tissues [11], and 56 many other areas [12-14]. But only one study so far used a combination of LCM and mass 57 spectrometry (MS) to localize unique proteins, potential biomarkers, when dealing with the 58 heterogeneity of breast tumor [15]. 59 LCM has also been used in parasitology, especially in sample preparation. Recent 60 studies employed this method in...

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Mass spectrometry imaging: a new vision in differentiatingSchistosoma mansonistrains

Cited by 30 publications

References 35 publications

Mass Spectrometry and Metabolomics—New Approaches for Helminth Biochemical Studies

Mass Spectrometry and Metabolomics—New Approaches for Helminth Biochemical Studies

The metabolic control of schistosome egg production

Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes ofEudiplozoon nipponicum(Monogenea, Polyopisthocotylea)

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