Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene

Lim, Heejin; Lee, Sun Young; Park, Yereum; Jin, Hyeonggyu; Seo, Daeha; Jang, Yun Hee; Moon, Dae Won

doi:10.1038/s41592-020-01055-6

Cited by 21 publications

(21 citation statements)

References 23 publications

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“…Multimodal imaging-based diagnosis is the most reliable technique to identify the problems in cancer- and bacteria-infected patients, and it will be helpful to surgeons and clinicians to make better predictions and conclusions about the problem, thus will improve treatment confidence. Several imaging techniques have been adopted in the research, such as CLSM, MRI, CT, PET, PAI, Raman, ToF-SIMS, and other imaging techniques for early diagnosis ( Scheme 3 ) [ 82 , 83 , 84 , 85 ].…”

Section: Graphene Nanocomposite Theranostics For Multimodal Imaging G...mentioning

confidence: 99%

“…Choi et al, Yang et al, Mirrahimi et al, Hong et al, Jia et al, Yang et al, Belu et al, and Lim et al demonstrated multimodal imaging (CLSM, MRI, CT, PET, PAI, RAMAN and ToF-SIMS) and multimodal therapies including chemo, gene, and PTs. Most of these researchers have also given tremendous efforts to improve the therapeutic capabilities by minimizing GNCs concentration, laser wavelengths from the first biological windows to the second biological window, and less laser powers and irradiation times [ 84 , 97 , 107 , 108 , 119 , 120 , 132 , 139 , 145 , 146 ]. Keshav et al, provided excellent pharmacokinetic data to take the material towards preclinical trials.…”

Section: Graphene Nanocomposite Theranostics For Multimodal Imaging G...mentioning

confidence: 99%

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Multimodal Imaging and Phototherapy of Cancer and Bacterial Infection by Graphene and Related Nanocomposites

2022

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The advancements in nanotechnology and nanomedicine are projected to solve many glitches in medicine, especially in the fields of cancer and infectious diseases, which are ranked in the top five most dangerous deadly diseases worldwide by the WHO. There is great concern to eradicate these problems with accurate diagnosis and therapies. Among many developed therapeutic models, near infra-red mediated phototherapy is a non-invasive technique used to invade many persistent tumors and bacterial infections with less inflammation compared with traditional therapeutic models such as radiation therapy, chemotherapy, and surgeries. Herein, we firstly summarize the up-to-date research on graphene phototheranostics for a better understanding of this field of research. We discuss the preparation and functionalization of graphene nanomaterials with various biocompatible components, such as metals, metal oxides, polymers, photosensitizers, and drugs, through covalent and noncovalent approaches. The multifunctional nanographene is used to diagnose the disease with confocal laser scanning microscopy, magnetic resonance imaging computed tomography, positron emission tomography, photoacoustic imaging, Raman, and ToF-SMIS to visualize inside the biological system for imaging-guided therapy are discussed. Further, treatment of disease by photothermal and photodynamic therapies against different cancers and bacterial infections are carefully conferred herein along with challenges and future perspectives.

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Section: Graphene Nanocomposite Theranostics For Multimodal Imaging G...mentioning

confidence: 99%

Section: Graphene Nanocomposite Theranostics For Multimodal Imaging G...mentioning

confidence: 99%

Multimodal Imaging and Phototherapy of Cancer and Bacterial Infection by Graphene and Related Nanocomposites

2022

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“…The graphene layer can then be removed by air plasma treatment, and ion images can be taken with an improved quality compared to air drying. Most recently, Lim et al (2021) showed that cells can be imaged in the wet state by covering them with grapheme, and that ions are sputtered through a transient hole while cells are still alive. Another possibility of sample preparation is to chemically fix cells with aldehydes and embed them in a resin (epoxy or acrylic resins), which has been commonly used for NanoSIMS.…”

Section: Secondary Ion Mass Spectrometry Imagingmentioning

confidence: 99%

Chemical Imaging and Analysis of Single Nerve Cells by Secondary Ion Mass Spectrometry Imaging and Cellular Electrochemistry

Lork

Phan

2022

Front. Synaptic Neurosci.

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A nerve cell is a unit of neuronal communication in the nervous system and is a heterogeneous molecular structure, which is highly mediated to accommodate cellular functions. Understanding the complex regulatory mechanisms of neural communication at the single cell level requires analytical techniques with high sensitivity, specificity, and spatial resolution. Challenging technologies for chemical imaging and analysis of nerve cells will be described in this review. Secondary ion mass spectrometry (SIMS) allows for non-targeted and targeted molecular imaging of nerve cells and synapses at subcellular resolution. Cellular electrochemistry is well-suited for quantifying the amount of reactive chemicals released from living nerve cells. These techniques will also be discussed regarding multimodal imaging approaches that have recently been shown to be advantageous for the understanding of structural and functional relationships in the nervous system. This review aims to provide an insight into the strengths, limitations, and potentials of these technologies for synaptic and neuronal analyses.

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“…Ron M. A. Heeren M olecular imaging mass spectrometry (MS) has taken another leap into the future. The group of Dae Won Moon at the Daegu Gyeongbuk Institute of Science & Technology in South Korea has developed an innovative method that allows the study of living cells inside the vacuum of an imaging ion source 1 . Their secret: using a graphene layer as molecular cellophane to protect living cells from the harsh vacuum environment (Fig.…”

Section: Molecular Cellophanementioning

confidence: 99%

“…Many will start using imaging MS to study the local dynamics of life without the need for labels -something that has not been possible to date. Lim et al 1 describe the details of this innovative approach in a paper published in this issue of Nature Methods.…”

Section: Molecular Cellophanementioning

confidence: 99%

Molecular cellophane

Heeren

2021

Nat Methods

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Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene

Cited by 21 publications

References 23 publications

Multimodal Imaging and Phototherapy of Cancer and Bacterial Infection by Graphene and Related Nanocomposites

Multimodal Imaging and Phototherapy of Cancer and Bacterial Infection by Graphene and Related Nanocomposites

Chemical Imaging and Analysis of Single Nerve Cells by Secondary Ion Mass Spectrometry Imaging and Cellular Electrochemistry

Molecular cellophane

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