Mass spectrometry in research laboratories and clinical diagnostic: a new era in medical mycology

Sarvestani, Hasti Kamali; Ramandi, Alireza; Getso, Muhammad Ibrahim; Razavyoon, Taraneh; Javidnia, Javad; Golrizi, Miaad Banay; Saboor-Yaraghi, Ali-Akbar; Ansari, Saham

doi:10.1007/s42770-022-00715-3

Cited by 6 publications

(2 citation statements)

References 161 publications

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“…The presumptive identification of isolates was based on standard criteria of macroscopic and microscopic morphologies, growth temperature, biochemical profile of aerobic sugar assimilation, and appearance on chromogenic agar ( Figure 1 ). All isolates were further processed for analysis with MALDI-TOF MS—VITEK MS (bioMérieux, Marcy l’Etoile, France) using VITEK MS v3.2 software [ 17 ]. All identifications displaying a single result with a confidence value of 99.9% were considered acceptable ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

Insights into Candida Colonization in Intensive Care Unit Patients: A Prospective Multicenter Study

Nascimento,

Inácio,

Guerreiro

et al. 2024

JoF

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The skin mycobiota plays a significant role in infection risk, pathogen transmission, and personalized medicine approaches in intensive care settings. This prospective multicenter study aimed to enhance our understanding of intensive care units’ (ICUs’) Candida colonization dynamics, identify modifiable risk factors, and assess their impact on survival risk. Specimens were taken from 675, 203, and 110 patients at the admission (D1), 5th (D5), and 8th (D8) days of ICU stay, respectively. The patient’s demographic and clinical data were collected. Candida isolates were identified by conventional culture-based microbiology combined with molecular approaches. Overall, colonization was 184/675 (27.3%), 87/203 (42.8%), and 58/110 (52.7%) on D1, D5, and D8, respectively. Candida colonization dynamics were significantly associated with ICU type (odds ratio (OR) = 2.03, 95% CI 1.22–3.39, p = 0.007), respiratory infection (OR = 1.74, 95% CI 1.17–2.58, p = 0.006), hemodialysis (OR = 2.19, 95% CI 1.17–4.10, p = 0.014), COVID-19 (OR = 0.37, 95% CI 0.14–0.99, p = 0.048), and with a poor 3-month outcome (p = 0.008). Skin Candida spp. colonization can be an early warning tool to generate valuable insights into the epidemiology, risk factors, and survival rates of critically ill patients, and should be considered for epidemiological surveillance.

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Section: Methodsmentioning

confidence: 99%

Insights into Candida Colonization in Intensive Care Unit Patients: A Prospective Multicenter Study

Nascimento,

Inácio,

Guerreiro

et al. 2024

JoF

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“…The library database plays the most crucial role in the MALDI-TOF MS system's ability to successfully identify organisms down to the species level. 10 Recent research has demonstrated that, under certain standardized protocols being developed for these microbes, MALDI-TOF MS may also be able to reliably identify filamentous fungus and dermatophytes. Additionally, MALDI-TOF MS has been effectively applied to subspecies microbiological typing and identification, indicating that this method has the potential to be an effective tool for taxonomical categorization and epidemiological research.…”

Section: Clinical Diagnosticsmentioning

confidence: 99%

Mass spectrometry in clinical laboratory: applications and future prospects

Ghonaim,

Bakr,

Alturki

et al. 2023

Int J Community Med Public Health

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Innovations in laboratory medicine face additional challenges due to the unmet need for extremely precise methods of disease diagnosis. Developments in mass spectrometry-based disease biomarker identification are constantly expanding the field of clinical diagnosis. More mass spectrometry-based in vitro diagnostics are anticipated to move from the bench to the bedside shortly, although several are currently incorporated into standard clinical procedures. The molecular detection by mass spectrometry technique is very effective in diagnosis of the disease and therapeutic monitoring because of its extremely high sensitivity, specificity, and short turnaround time. Mass spectrometry is a potent analytical instrument that may be used to analyse a variety of materials and matrices; its use in clinical laboratory medicine applications is rising. For clinical prognostics and diagnostic purposes, mass spectrometry imaging has been widely utilized to distinguish between healthy and diseased tissues. Modern single-cell studies will benefit from its cutting-edge applications, which will offer comprehensive cellular biochemical data for mechanistic comprehension and, eventually, the development of therapeutic interventions. Within the field of laboratory medicine, the application of mass spectrometry is crucial, especially in terms of diagnostics and therapeutic drug monitoring. This review on the clinical application of mass spectrometry and its future prospects promises an insightful exploration of the evolving landscape of mass spectrometry in clinical settings, delving into its current applications and shedding light on its potential future advancements, for which a comprehensive search in the PubMed, ScienceDirect, and Web of Science databases was conducted.

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Spermatozoa and seminal plasma proteomics: Too many molecules, too few markers. The case of bovine and porcine semen

Bustamante‐Filho

Pasini

Moura

2022

Animal Reproduction Science

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Mass spectrometry in research laboratories and clinical diagnostic: a new era in medical mycology

Cited by 6 publications

References 161 publications

Insights into Candida Colonization in Intensive Care Unit Patients: A Prospective Multicenter Study

Insights into Candida Colonization in Intensive Care Unit Patients: A Prospective Multicenter Study

Mass spectrometry in clinical laboratory: applications and future prospects

Spermatozoa and seminal plasma proteomics: Too many molecules, too few markers. The case of bovine and porcine semen

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