Mass Spectrometry of Nucleotides and Oligonucleotides

Td, McClure; Kh, Schram

doi:10.1007/978-1-59259-513-6_13

Cited by 1 publication

(1 citation statement)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first studies using fast atom bombardment (FAB) or liquid secondary ion mass spectrometry (LSIMS) were limited to small oligonucleotides (4; < 5000 Da). With the development of new methods of ionization such as electrospray ionization (ESI; 5) or matrix-assisted laser desorption ionization (MALDI; [6][7][8], which can produce intact gaseous ions from highly polar molecules, it has become possible to analyze higher molecular weight oligonuleotides (> 10 000 Da) with a precision comparable with that obtained for proteins (9,10).…”

Section: Introductionmentioning

confidence: 99%

Negative electrospray ionization mass spectrometry of synthetic and chemically modified oligonucleotides

Potier¹,

Dorsselaer²,

et al. 1994

View full text Add to dashboard Cite

We report here on the analysis of synthetic oligonucleotides by electrospray ionization mass spectrometry (ESI-MS). After intensive removal of salt ions (especially sodium cations), negative ion mass spectra, allowing mass measurement with an accuracy of 0.01%, were obtained on several oligonucleotides up to 80 nucleotides. In most cases, the resolution was sufficient to observe n-1 and n-2 forms due to internal deletions during automated synthesis, and to identify the missing nucleotides. A 132-mer, whose size is close to the limit of automated chemical synthesis, was also successfully mass measured. A quantitative study showed that ESI-MS can provide quantitative data on oligonucleotides of similar size and structure. The described methodology is used to characterize oligonucleotide analogues such as phosphorothioate oligonucleotides designed for antisense applications. Finally, analyses in the positive ion mode on a trimer TpTpT in the presence of different amine bases were performed and allowed a better understanding of the influence of these bases on the ions formation.

show abstract