Mass spectrometry of triacylglycerols

Laakso, Päivi

doi:10.1002/1438-9312(200201)104:1<43::aid-ejlt43>3.0.co;2-j

Cited by 44 publications

(14 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…). These neutral molecules were not protonated in DESI, but instead formed [M + NH 4 ] + ions with the ammonia‐containing solvents 4 and 5 and [M + Na] + ions with the other spray solvents, in agreement with earlier results with ESI and DESI . Tributyrin also produced a fragment ion at m / z 215 by the loss of one fatty acid chain (Fig.…”

Section: Resultssupporting

confidence: 89%

“…The [M‐H] + ions of the vitamins were likely formed by the loss of a hydrogen molecule from the [M + H] + . The triacylglycerols, however, had insufficient PAs for proton transfer reaction, but they could be ionized via ammonium adduct or sodium adduct formation, like in ESI . The addition of ammonium acetate (solvent 4) or ammonium hydroxide in the spray solvent (solvent 5) favored the formation of [M + NH 4 ] + instead of [M + Na] + , simplifying the spectra.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of lipids with desorption atmospheric pressure photoionization‐mass spectrometry (DAPPI‐MS) and desorption electrospray ionization‐mass spectrometry (DESI‐MS)

et al. 2012

View full text Add to dashboard Cite

In this article, the effect of spray solvent on the analysis of selected lipids including fatty acids, fat-soluble vitamins, triacylglycerols, steroids, phospholipids, and sphingolipids has been studied by two different ambient mass spectrometry (MS) methods, desorption electrospray ionization-MS (DESI-MS) and desorption atmospheric pressure photoionization-MS (DAPPI-MS). The ionization of the lipids with DESI and DAPPI was strongly dependent on the spray solvent. In most cases, the lipids were detected as protonated or deprotonated molecules; however, other ions were also formed, such as adduct ions (in DESI), [M-H](+) ions (in DESI and DAPPI), radical ions (in DAPPI), and abundant oxidation products (in DESI and DAPPI). DAPPI provided efficient desorption and ionization for neutral and less polar as well as for ionic lipids but caused extensive fragmentation for larger and more labile compounds because of a thermal desorption process. DESI was more suitable for the analysis of the large and labile lipids, but the ionization efficiency for less polar lipids was poor. Both methods were successfully applied to the direct analysis of lipids from pharmaceutical and food products. Although DESI and DAPPI provide efficient analysis of lipids, the multiple and largely unpredictable ionization reactions may set challenges for routine lipid analysis with these methods.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Analysis of lipids with desorption atmospheric pressure photoionization‐mass spectrometry (DAPPI‐MS) and desorption electrospray ionization‐mass spectrometry (DESI‐MS)

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Nevertheless, TAG can be analyzed together with trimethylsilylated DAG and MAG and even further lipid classes, such as CE, free FA and others, within one single run on the same capillary column 42. In combination with MS the molecular species can be well identified 43. For further separation, more polar phases are necessary.…”

Section: Gcmentioning

confidence: 99%

Determination of acylglycerols from biological samples with chromatography‐based methods

Hellmuth¹,

Uhl²,

Segura³

et al. 2011

J of Separation Science

View full text Add to dashboard Cite

Lipids are the most diverse class of metabolites in mammalian physiology and dysregulation of lipid metabolism is linked to various diseases. Alterations in acylglycerols, a major class of lipids in plasma and adipose tissue, are involved in the pathogenesis of obesity and type 2 diabetes. Therefore, determination of acylglycerols is important to depict and unravel cellular mechanisms related to pathological outcomes, and specific molecular species of acylglycerols might be promising biomarker candidates. The variety of acylglycerols can be characterized in different ways. Enzymatic assays enable the determination of total tri- or diacylglycerols showing a possible relation to diseases, but they do not allow clarification of molecular mechanism. While gas chromatography can provide an overview of the fatty acid composition of total or separated lipids, a very detailed description of the individual molecular acylglycerol species is possible via liquid chromatography, particularly when combined with mass spectrometry. This review describes the determination of acylglycerols considering recent developments, with a focus on mammalian serum/plasma and tissue.

show abstract

“…To study the trace amounts of glycerolipids extracted from biological samples, several analytical methods have been pursued 3–6. Mass spectrometry (MS) is an important analytical tool for the structural study of compounds extracted from trace amounts of biological samples.…”

mentioning

confidence: 99%

Structural determination of monoacylglycerols extracted from marine sponge by fast atom bombardment tandem mass spectrometry

Gil

Hong

Jung

et al. 2007

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Five new monoacylglycerols (MAGs) were isolated from the marine sponge Stelletta sp. by reversed-phase high-performance liquid chromatography and analyzed by positive ion fast atom bombardment mass spectrometry (FAB-MS). FAB mass spectra of these compounds produced abundant sodium-adducted molecules [M+Na]+ from a mixture of 3-nitrobenzyl alcohol and sodium iodide. The structural elucidation of these sponge MAGs was carried out by FAB tandem mass spectrometry (MS/MS). To find diagnostic ions for the characterization of the MAGs, authentic MAGs were initially analyzed by collision-induced dissociation (CID) MS/MS. The CID MS/MS of [M+Na]+ precursor ions resulted in the formation of numerous characteristic product ions via a series of dissociative processes. The product ions formed by charge-remote fragmentation (CRF) provided important information for the characterization of acyl chains substituted at the glycerol backbone, and product ions at m/z 84, 97, 113 and 139 were diagnostic for the sodiated glycerol backbone. On the basis of these fragmentation patterns, the structures of five MAGs extracted from marine sponge were elucidated. In addition, high-resolution mass measurement was performed to obtain the elemental compositions of the MAGs.

show abstract

Mass spectrometry of triacylglycerols

Cited by 44 publications

References 46 publications

Analysis of lipids with desorption atmospheric pressure photoionization‐mass spectrometry (DAPPI‐MS) and desorption electrospray ionization‐mass spectrometry (DESI‐MS)

Analysis of lipids with desorption atmospheric pressure photoionization‐mass spectrometry (DAPPI‐MS) and desorption electrospray ionization‐mass spectrometry (DESI‐MS)

Determination of acylglycerols from biological samples with chromatography‐based methods

Structural determination of monoacylglycerols extracted from marine sponge by fast atom bombardment tandem mass spectrometry

Contact Info

Product

Resources

About