Mass transport controlled oxygen reduction at anthraquinone modified 3D-CNT electrodes with immobilized Trametes hirsuta laccase

Abstract

Carbon nanotubes covalently modified with anthraquinone were used as an electrode for the immobilization of Trametes hirsuta laccase. The adsorbed laccase is capable of oxygen reduction at a mass transport controlled rate (up to 3.5 mA cm(-2)) in the absence of a soluble mediator. The storage and operational stability of the electrode are excellent.

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

“…[251] Many other works report the mixture of different materials to modulate the porosity for electroenzymatic reactions, increasing at the same time the conductivity of the material: Ag NP and CNTs, [252] reticulated vitreous carbon mixed with CNTs, [253] and CFs dipped in CNT dispersions. [209,254,255] Catalytic currents were increased by the addition of CNTs, but, surprisingly, some discrepancy on DET or MET processes exist between the works. Fine physicochemical characterization of the network is still needed, which requires interdisciplinary collaboration between biochemists, bioelectrochemists, and material chemists to improve our understanding of the parameters that determine efficient catalysis at 3D electrodes.…”

Supramolecular Immobilization of Laccase on Carbon Nanotube Electrodes Functionalized with (Methylpyrenylaminomethyl)anthraquinone for Direct Electron Reduction of Oxygen

“…Different polycyclic molecules were found to enter this cavity, strongly interact with laccase to achieve efficient DET and stabilize electroenzymatic activity over days 7. Recently, covalent modification of carbon nanotube (CNT) electrodes with polyaromatics such as anthraquinone groups has been achieved by different covalent approaches: amination of CNT nanofibers,8 diazonium chemistry9–11 and amidification of carboxylic CNT defects 12…”

Wiring Laccase on Covalently Modified Graphene: Carbon Nanotube Assemblies for the Direct Bio‐electrocatalytic Reduction of Oxygen

“…The aryldiazonium reduction process, as previously developed for carbon nanotubes, has already demonstrated its efficiency and flexibility for functionalizing CVDgrown graphene [11,12] or RGO. We and others recently adapted this technique by non-covalent [22][23][24][25] or covalent functionalization [26][27][28][29] of carbon nanotubes. As the direct electrochemistry of redox proteins is particularly investigated in the field of biosensing and biofuel cells, [15] only few works have reported the direct electrochemistry of redox proteins on graphene-based materials.…”

“…[16] These examples have only focused on glucose oxidases [17] or heme-containing metalloproteins, such as horseradish peroxidases, cytochrome c, or hemoglobin. We and others recently adapted this technique by non-covalent [22][23][24][25] or covalent functionalization [26][27][28][29] of carbon nanotubes. [20,21] As the T1 copper center is involved in the electron transfer between the electrode and the T2/T3 copper binding sites, efficient direct oxygen reduction electrocatalysis was demonstrated.…”