Adult Hookworms usually live in the duodenum and jejunum and can be recovered endoscopically for the diagnosis of chronic anaemia. This report describes an interesting case where adult hookworm (Ancylostoma duodenale) was recovered from the terminal ileum by colonoscopy in an old female patient suffering from chronic severe anemia. Her upper gastro intestinal endoscopic findings were normal and fecal occult blood test was positive. The colonoscopic finding was further confirmed by the presence of characteristic eggs of hookworm in stool microscopy and she was treated with anthelminthic along with symptomatic measures. Her clinical condition as well as the blood profile showed much improvement after treatment. Thus, colonoscopy should be considered for the presence of hookworms if the upper endoscopic findings are normal in a clinically suspected patient. J Microbiol Infect Dis 2017; 7(2): 98-100