Mast cell and heparin promote adipogenesis in superficial fascia of rats

Chen, Tongsheng; Zhang, Yanfei; Dong, Yingyue; Zhang, Dandan; Xia, Lisha; Sun, Xiaozhe; Li, Hanxiao; Han, Chunmiao; Wang, Huamin; Gao, Xu

doi:10.1016/j.bbalip.2021.159024

Cited by 9 publications

(20 citation statements)

References 30 publications

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“…Upon high-fat diet feeding, epididymal WAT expands through both adipocyte hyperplasia and hypertrophy, whereas inguinal WAT expands predominantly through hypertrophy and undergoes a low rate of adipocyte proliferation [ 31 ]. By contrast, both Ing-ADSCs and Epi-ADSCs cultures proliferate and robustly differentiate into adipocytes in vitro [ 47 , 48 , 49 , 50 , 51 ]. Currently, the inhibition tendencies of metformin are similar in both Ing-ADSCs and Epi-ADSCs, including the distribution of lipid droplet size, triglyceride deposition, levels of adipogenic transcription factors (Pparγ and Cebpα), and adipokines (Fabp4 and adiponectin).…”

Section: Discussionmentioning

confidence: 99%

Metformin Inhibits Lipid Droplets Fusion and Growth via Reduction in Cidec and Its Regulatory Factors in Rat Adipose-Derived Stem Cells

Yang

Jia

Fang

et al. 2022

IJMS

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Metformin is still being investigated due to its potential use as a therapeutic agent for managing overweight or obesity. However, the underlying mechanisms are not fully understood. Inhibiting the adipogenesis of adipocyte precursors may be a new therapeutic opportunity for obesity treatments. It is still not fully elucidated whether adipogenesis is also involved in the weight loss mechanisms by metformin. We therefore used adipose-derived stem cells (ADSCs) from inguinal and epididymal fat pads to investigate the effects and mechanisms of metformin on adipogenesis in vitro. Our results demonstrate the similar effect of metformin inhibition on lipid accumulation, lipid droplets fusion, and growth in adipose-derived stem cells from epididymal fat pads (Epi-ADSCs) and adipose-derived stem cells from inguinal fat pads (Ing-ADSCs) cultures. We identified that cell death-inducing DFFA-like effector c (Cidec), Perilipin1, and ras-related protein 8a (Rab8a) expression increased ADSCs differentiation. In addition, we found that metformin inhibits lipid droplets fusion and growth by decreasing the expression of Cidec, Perilipin1, and Rab8a. Activation of AMPK pathway signaling in part involves metformin inhibition on Cidec, Perilipin1, and Rab8a expression. Collectively, our study reveals that metformin inhibits lipid storage, fusion, and growth of lipid droplets via reduction in Cidec and its regulatory factors in ADSCs cultures. Our study supports the development of clinical trials on metformin-based therapy for patients with overweight and obesity.

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Section: Discussionmentioning

confidence: 99%

Metformin Inhibits Lipid Droplets Fusion and Growth via Reduction in Cidec and Its Regulatory Factors in Rat Adipose-Derived Stem Cells

Yang

Jia

Fang

et al. 2022

IJMS

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“…According to our laboratory-established method [ 13 , 18 , 19 ], whole-mounted superficial fascia was adhered to the glass slides and fixed by 4% paraformaldehyde for 0.6 h at room temperature. For conventional staining, fascial adipocytes were stained with Oil-red O (0.5%) for 20 min or Nile Red (1 μg/mL) for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…FSCs were isolated in accordance with our previously established methods [ 13 , 18 , 19 ]. Fascia was minced into 1- to 3-mm 3 pieces, digested by type I collagenase (0.8 mg/mL) at 37°C, then shaken for 2 h at 140 cycles/min.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, we proposed a novel theory of the fascial origin of adipocytes [ 13 , 17 ]. We identified that mast cells and their granule heparin act as endogenous regulatory factors that initiate fascial adipogenesis [ 18 ], and also, we revealed that fascial adipocytes showed unique cytological and functional properties that maintained high basal lipolysis but were not sensitive to catecholamines as compared with subcutaneous and visceral adipocytes [ 19 ]. These results indicate that adipose cells can originate from the superficial fascia, which represents a novel origin of adipocytes with different cytological, functional and developmental features [ 13 , 17–19 ].…”

Section: Introductionmentioning

confidence: 99%

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Generation of functional fat organoid from rat superficial fascia

Zhang

Dong

et al. 2022

Adipocyte

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The organoid is a 3D cell architecture formed by self-organized tissues or cells in vitro with similar cell types, histological structures, and biological functions of the native organ. Depending on the unique organ structures and cell types, producing organoids requires individualized design and is still challenging. Organoids of some tissues, including adipose tissue, remain to generate to be more faithful to their original organ in structure and function. We previously established a new model of the origin of adipose cells originating from non-adipose fascia tissue. Here, we investigated superficial fascia fragments in 3D hydrogel and found they were able to transform into relatively large adipocyte aggregates containing mature unilocular adipocytes, which were virtually “fat organoids”. Such fascia-originated fat organoids had a typical structure of adipose tissues and possessed the principal function of adipose cells in the synthesis, storage, hydrolysis of triglycerides and adipokines secretion. Producing fat organoids from superficial fascia can provide a new approach for adipocyte research and strongly evidences that both adipose tissues and cells originate from fascia. Our findings give insights into metabolic regulation by the crosstalk between different organs and tissues and provide new knowledge for investigating novel treatments for obesity, diabetes and other metabolic diseases. Abbreviations : 3D: three dimensional; ASC: adipose-derived stromal cells; C/EBP: CCAAT-enhancer-binding protein; EdU: 5-ethynyl-2-deoxyuridine; FABP4: fatty acid-binding protein 4; FAS: fatty acid synthase; FSCs: fascia-derived stromal cells; Plin1: perilipin-1; Plin2: perilipin-2; PPARγ: peroxisome proliferator-activated receptor γ; WAT: white adipose tissue

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“…Furthermore, Chen et al. demonstrated that mast cell derived heparin could act as the endogenous factor initiating fascial adipogenesis ( 81 ). These findings suggest an adipose depot specific effect of mast cells on adipocyte progenitors, dependent on other immune cells and the metabolic state of the organism.…”

Section: Interaction Of Preadipocytes With Other Stromal Cellsmentioning

confidence: 99%

Immune Cell Regulation of White Adipose Progenitor Cell Fate

2022

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Adipose tissue is essential for energy storage and endocrine regulation of metabolism. Imbalance in energy intake and expenditure result in obesity causing adipose tissue dysfunction. This alters cellular composition of the stromal cell populations and their function. Moreover, the individual cellular composition of each adipose tissue depot, regulated by environmental factors and genetics, determines the ability of the depots to expand and maintain its endocrine and storage function. Thus, stromal cells modulate adipocyte function and vice versa. In this mini-review we discuss heterogeneity in terms of composition and fate of adipose progenitor subtypes and their interactions with and regulation by different immune cell populations. Immune cells are the most diverse cell populations in adipose tissue and play essential roles in regulating adipose tissue function via interaction with adipocytes but also with adipocyte progenitors. We specifically discuss the role of macrophages, mast cells, innate lymphoid cells and T cells in the regulation of adipocyte progenitor proliferation, differentiation and lineage commitment. Understanding the factors and cellular interactions regulating preadipocyte expansion and fate decision will allow the identification of novel mechanisms and therapeutic strategies to promote healthy adipose tissue expansion without systemic metabolic impairment.

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Mast cell and heparin promote adipogenesis in superficial fascia of rats

Cited by 9 publications

References 30 publications

Metformin Inhibits Lipid Droplets Fusion and Growth via Reduction in Cidec and Its Regulatory Factors in Rat Adipose-Derived Stem Cells

Metformin Inhibits Lipid Droplets Fusion and Growth via Reduction in Cidec and Its Regulatory Factors in Rat Adipose-Derived Stem Cells

Generation of functional fat organoid from rat superficial fascia

Immune Cell Regulation of White Adipose Progenitor Cell Fate

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