Mast Cell Density in Oral Lesions using Metachromatic Stains: A Comparative Study

Mast cell expression in oral lichen planus: A systematic review

“…All 12 studies had answered the 1st research question of the mast cell number in OLP patients. 5 studies had used toluidine blue‐based staining to count the number of the mast cells. 4 studies had used anti‐tryptase antibody staining to detect the mast cells.…”

“…4 studies had used anti‐tryptase antibody staining to detect the mast cells. 1 study by Gholamreza et al had compared the mast cell numbers from both toluidine blue staining and anti‐tryptase antibody‐based staining, and another unique study by Balci et al examined the mast cell counts by toluidine blue staining with CD1177 (C‐Kit) methods.…”

“…1 study had qualitatively stated that there is an increase in mast cell density and had not reported on the absolute numbers. 5 studies had compared the mast cell numbers with OLR, 2 studies had reported that there is an increase in the mast cells in OLR compared with OLP, 2 studies had reported that the mast cell numbers were higher in OLP and 1 study reported no significant difference . 1 study had compared the mast cell numbers with OLR and squamous cell carcinoma and stated that the mast cell numbers were highest in OLP.…”

“…A total of 4 studies had used an ocular grid to count the mast cell numbers in sequential fields. 4 studies had counted in 5 sequential high‐power fields, and 1 study had used 10 high‐power fields . A study by Muniz et al had used an ocular micrometer to calculate the area of each examination field and had reported that mast cell numbers in terms of density (no.…”

“…In terms of mast cell number, 10 studies [12][13][14]17,18,21,22 had quantitively reported an increase in mast cell numbers in OLP compared with normal. 1 study 22 had qualitatively stated that there is an increase in mast cell density and had not reported on the absolute numbers.…”