Mast cell–derived IL-13 downregulates IL-12 production by skin dendritic cells to inhibit the TH1 cell response to cutaneous antigen exposure

Leyva-Castillo, Juan-Manuel; Das, Mrinmoy; Artru, Emilie; Yoon, Jeong‐Won; Galand, C

doi:10.1016/j.jaci.2020.11.036

Cited by 41 publications

(30 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon stimulation with IL-33, BMMC and FSMC showed a comparably high increase in fold change for Il13, a MC-derived type 2 immune cytokine, while FLMC showed a significantly lower expression (Figure 2C). This result confirmed others e.g., by Leyva-Castillo et al, who showed IL-33-dependent upregulation of Il13 expression in skin MC [30]. To test the functional relevance of the ST2 expression further, MC were stimulated with 10 ng/mL IL-33 for 24 h and type 2 specific cytokines were measured in the supernatant.…”

Section: Phenotypes Following Cultures Of Bone Marrow Cells and Fetal...supporting

confidence: 87%

Fetal Tissue-Derived Mast Cells (MC) as Experimental Surrogate for In Vivo Connective Tissue MC

Iuliano

Absmaier-Kijak

Sinnberg

et al. 2022

Cells

View full text Add to dashboard Cite

Bone-marrow-derived mast cells are matured from bone marrow cells in medium containing 20% fetal calf serum (FCS), interleukin (IL)-3 and stem-cell factor (SCF) and are used as in vitro models to study mast cells (MC) and their role in health and disease. In vivo, however, BM-derived hematopoietic stem cells account for only a fraction of MC; the majority of MC in vivo are and remain tissue resident. In this study we established a side-by-side culture with BMMC, fetal skin MC (FSMC) or fetal liver MC (FLMC) for comparative studies to identify the best surrogates for mature connective tissue MC (CTMC). All three MC types showed comparable morphology by histology and MC phenotype by flow cytometry. Heterogeneity was detected in the transcriptome with the most differentially expressed genes in FSMC compared to BMMC being Hdc and Tpsb2. Expression of ST2 was highly expressed in BMMC and FSMC and reduced in FLMC, diminishing their secretion of type 2 cytokines. Higher granule content, stronger response to FcεRI activation and significantly higher release of histamine from FSMC compared to FLMC and BMMC indicated differences in MC development in vitro dependent on the tissue of origin. Thus, tissues of origin imprint MC precursor cells to acquire distinct phenotypes and signatures despite identical culture conditions. Fetal-derived MC resemble mature CTMC, with FSMC being the most developed.

show abstract

Section: Phenotypes Following Cultures Of Bone Marrow Cells and Fetal...supporting

confidence: 87%

Fetal Tissue-Derived Mast Cells (MC) as Experimental Surrogate for In Vivo Connective Tissue MC

Iuliano

Absmaier-Kijak

Sinnberg

et al. 2022

Cells

View full text Add to dashboard Cite

show abstract

“…Depending on the route of activation, MCs can bridge innate and adaptive immunity and thereby contribute to allergic sensitization in the skin and lung [ 215 ]. For example, MC-derived IL-13 has been demonstrated to downregulate IL-12 production by skin DCs, thereby inhibiting the T H 1 cell response to cutaneous antigen exposure [ 216 ]. Likewise, MCs counter-regulate IFN-γ expression in sensitized skin [ 217 ].…”

Section: Mast Cell Contribution To Inflammatory Skin Disordersmentioning

confidence: 99%

Mast Cells in the Skin: Defenders of Integrity or Offenders in Inflammation?

Voß

Kotrba

Gaffal

et al. 2021

IJMS

View full text Add to dashboard Cite

Mast cells (MCs) are best-known as key effector cells of immediate-type allergic reactions that may even culminate in life-threatening anaphylactic shock syndromes. However, strategically positioned at the host–environment interfaces and equipped with a plethora of receptors, MCs also play an important role in the first-line defense against pathogens. Their main characteristic, the huge amount of preformed proinflammatory mediators embedded in secretory granules, allows for a rapid response and initiation of further immune effector cell recruitment. The same mechanism, however, may account for detrimental overshooting responses. MCs are not only detrimental in MC-driven diseases but also responsible for disease exacerbation in other inflammatory disorders. Focusing on the skin as the largest immune organ, we herein review both beneficial and detrimental functions of skin MCs, from skin barrier integrity via host defense mechanisms to MC-driven inflammatory skin disorders. Moreover, we emphasize the importance of IgE-independent pathways of MC activation and their role in sustained chronic skin inflammation and disease exacerbation.

show abstract

“…Nonetheless, IL-33 also plays a protective role in worm infection and wound healing [ 55 , 56 ]. TNF α-2β can inhibit the proliferation of MCs [ 57 ]. TGF-β1 promotes MCs to secrete pro-inflammatory cytokines and IL-13, which induces inflammation and fibrosis [ 58 ].…”

Section: Pro-allergic and Inflammatory Actions Of Mcsmentioning

confidence: 99%

“…Meanwhile, some studies have shown that IL-33 can promote the secretion of IL-13 by MCs, which inhibits the production of IL-12 by DCs in the skin. This can hinder the Th1 cell response to cutaneous antigen exposure [ 57 ].…”

Section: Regulatory-type Actions Of Mcs In Allergy and Inflammationmentioning

confidence: 99%

Two Sides of the Coin: Mast Cells as a Key Regulator of Allergy and Acute/Chronic Inflammation

Zhang

Kurashima

2021

Cells

View full text Add to dashboard Cite

It is well known that mast cells (MCs) initiate type I allergic reactions and inflammation in a quick response to the various stimulants, including—but not limited to—allergens, pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs). MCs highly express receptors of these ligands and proteases (e.g., tryptase, chymase) and cytokines (TNF), and other granular components (e.g., histamine and serotonin) and aggravate the allergic reaction and inflammation. On the other hand, accumulated evidence has revealed that MCs also possess immune-regulatory functions, suppressing chronic inflammation and allergic reactions on some occasions. IL-2 and IL-10 released from MCs inhibit excessive immune responses. Recently, it has been revealed that allergen immunotherapy modulates the function of MCs from their allergic function to their regulatory function to suppress allergic reactions. This evidence suggests the possibility that manipulation of MCs functions will result in a novel approach to the treatment of various MCs-mediated diseases.

show abstract

Mast cell–derived IL-13 downregulates IL-12 production by skin dendritic cells to inhibit the TH1 cell response to cutaneous antigen exposure

Cited by 41 publications

References 45 publications

Fetal Tissue-Derived Mast Cells (MC) as Experimental Surrogate for In Vivo Connective Tissue MC

Fetal Tissue-Derived Mast Cells (MC) as Experimental Surrogate for In Vivo Connective Tissue MC

Mast Cells in the Skin: Defenders of Integrity or Offenders in Inflammation?

Two Sides of the Coin: Mast Cells as a Key Regulator of Allergy and Acute/Chronic Inflammation

Contact Info

Product

Resources

About