Mast Cell Differentiation in Cultures of T Cell-Depleted Mesenteric Lymph Node Cells from &lt;i&gt;Nippostrongylus brasiliensi&lt;/i&gt;&lt;i&gt;s&lt;/i&gt;-lnfected Mice

Huff, Thomas; Justus, David E.

doi:10.1159/000234492

Cited by 6 publications

(1 citation statement)

References 5 publications

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“…The latter could, perhaps, apply to the RCMC lines described here. As suggested by Huff and Justus [48], factor independence may, however, very with discrete stages of mast cell differentiation. Finally, the possibility that the RCMC lines were transformed, possibly by a virus, cannot be rigorously excluded.…”

Section: Discussionmentioning

confidence: 88%

Characterization of rat tissue cultured mast cells

Chan¹,

Hu³

et al. 1990

Eur J Immunol

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Twelve continuous rat tissue cultured mast cell (MC) lines were established by prolonged culture of rat peritoneal MC in the absence of added factors or feeder layers. Two of these lines, RCMC1 and RCMC2, have been briefly described previously, seven others are now also described. Both RCMC1 and RCMC2 lack a marker chromosomes present on RBL-CA10.7 cells. All lines were found to express the phenotype of mucosal MC as defined by alcian blue-positive and safranin O-negative staining, the presence of rat MC protease II and a low histamine content. When analyzed for high-(Fc epsilon RI) and low-affinity (Fc epsilon RL) receptors for IgE, the various lines yielded a variety of receptor patterns. Northern blot analysis of the RNA of RCMC1, RCMC2 and RBL-CA10.7 revealed that all three cell lines contained the same mRNA species for the alpha, beta and gamma subunits for Fc epsilon RI previously found in another rat basophilic leukemia cell line. Quantitation of the relative amounts of alpha, beta and gamma mRNA did not correlate with the expression of the relative amounts of Fc epsilon RI(alpha) in these cells. The relative amounts of mRNA for all these subunits of RCMC2 were equal or higher than those of RCMC1, suggesting that the low expression of Fc epsilon RI(alpha) on the former was a consequence of post-transcriptional events. Analysis of a RCMC1 clone over a 6-month period revealed changes in the expression of both Fc epsilon RI(alpha) and Fc epsilon RL.

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Section: Discussionmentioning

confidence: 88%

Characterization of rat tissue cultured mast cells

Chan¹,

Hu³

et al. 1990

Eur J Immunol

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Isolation and characterization of ige receptors from rat intestinal mucosal mast cells

et al. 1989

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High-(Fc epsilon RI) and low-(Fc epsilon RII) affinity IgE receptors were isolated from surface radioiodinated, Nonidet-P40-solubilized rat intestinal mucosal mast cells (IMMC) and compared with those on rat peritoneal mast cells (PMC) and rat basophilic leukemia (RBL) cells. Fc epsilon RII were isolated by affinity chromatography using IgE-Sepharose or by anti-Fc epsilon RII antisera and protein A-Sepharose. The surface-exposed, IgE-binding alpha subunits of Fc epsilon RI [Fc epsilon RI alpha] were isolated by affinity chromatography using IgE and anti-IgE-Sepharose. Fc epsilon RI alpha on IMMC had an apparent molecular mass of 59 kDa, somewhat larger than that of PMC (51 kDa), RBL-2H3 cells (51 kDa) or RBL-CA10.7 cells (46 kDa). Brief (45 s) incubation of IMMC or PMC in glycine-HCl, pH 3, prior to iodination removed much of the surface-bound IgE. This permitted more thorough labeling of the receptors, but had no affect on the estimate of receptor size. Surprisingly and in contrast to acid-treated PMC, upon anti-IgE-Sepharose isolation acid-treated IMMC yielded an intensely radioactive Fc epsilon RI alpha band in the absence of added IgE. Such a finding suggests that IMMC, more so than PMC, may have an intracellular store of IgE, as has been suggested by many others. IMMC also differed from PMC in the number of forms of Fc epsilon RII isolated; 50-kDa and 58-kDa forms of Fc epsilon RII were obtained from IMMC, whereas PMC yielded most often a single 56-kDa Fc epsilon RII band. These results were mimicked by the two RBL cell sublines: RBL-2H3 cells yielded two Fc epsilon RII (46 kDa and 55 kDa), but only one form of Fc epsilon RII (54-kDa) was obtained from RBL-CA10.7 cells. Thus, the two subtypes of rat mast cells, which have previously been shown to differ in mediator profile and responsiveness to secretagogues and antiallergic drugs, are also distinguished by differences in IgER profile.

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Role for the stem cell factor/KIT complex in schwann cell neoplasia and mast cell proliferation associated with neurofibromatosis

Ryan

Klein

Neuberger

et al. 1994

J of Neuroscience Research

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Schwann cells are the primary cell type in the disfiguring lesions associated with neurofibromatosis type 1 (NF-1). These lesions also contain abnormally high numbers of mast cells, a cell type which develops in response to stem cell factor. We report here that neonatal and adult rat and human Schwann cells, as well as a transfected rat Schwann cell line and a human Schwannoma line derived from an NF-1 patient, all produced stem cell factor mRNA and protein. In coculture experiments, surface expression of stem cell factor by neonatal rat Schwann cells was profoundly downregulated by contact with dorsal root ganglion neurites. The receptor for stem cell factor, KIT, was not expressed in normal Schwann cells but was expressed in the human Schwannoma line, suggesting that aberrant KIT expression may form an autocrine loop in certain Schwann cell neoplasias.

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Mast Cell Differentiation in Cultures of T Cell-Depleted Mesenteric Lymph Node Cells from <i>Nippostrongylus brasiliensi</i><i>s</i>-lnfected Mice

Cited by 6 publications

References 5 publications

Characterization of rat tissue cultured mast cells

Characterization of rat tissue cultured mast cells

Isolation and characterization of ige receptors from rat intestinal mucosal mast cells

Role for the stem cell factor/KIT complex in schwann cell neoplasia and mast cell proliferation associated with neurofibromatosis

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