Mast Cell Heterogeneity in Response to Cholinergic Stimulation

Masini, E.; Fantozzi, Roberto; Conti, Andrea A.; Blandina, Patrizio; Brunelleschi, Sandra; Mannaioni, P. F.

doi:10.1159/000233780

Cited by 23 publications

(14 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to bronchial smooth muscle cells, endothelial [19], epithelial [20], neutrophils [21], lymphocytes [22], mast cells [23] monocytes and macrophages [24] have been reported to possess muscarinic acetylcholine receptors. Furthermore, the data available do not distinguish whether the mediator response in BAL fluid is a direct effect of methacholine or of bronchoconstriction itself.…”

Section: Discussionmentioning

confidence: 99%

Methacholine challenge does not affect bronchoalveolar fluid cell number and many indices of cell function in asthma

et al. 1995

View full text Add to dashboard Cite

No significant differences were noted in any of these markers of cell number or function which could be ascribed to methacholine challenge.Thus, methacholine challenge does not appear to affect these markers of cell number and function. These findings indicate that a methacholine challenge can be used as a measure of bronchial hyperresponsiveness within 30 min prior to bronchoscopy without altering bronchoalveolar lavage fluid characteristics.

show abstract

Section: Discussionmentioning

confidence: 99%

Methacholine challenge does not affect bronchoalveolar fluid cell number and many indices of cell function in asthma

et al. 1995

View full text Add to dashboard Cite

show abstract

“…It is interesting to note that the earliest description of potential cholinerergic regulation of mast cells was not an inhibitory action at the nicotinic receptors but an activation and induction of degranulation by acetylcholine acting at the muscarinic receptors [107,108]. Mannaioni and colleagues [107] reported that histamine secretion from rat mast cells occurs in the presence of nanomolar concentrations of acetylcholine, an effect that was competitively blocked by atropine.…”

Section: Cholinergic Neuronsmentioning

confidence: 99%

“…However, other studies failed to show any effect of acetylcholine on rat mast cell degranulation [109,110]. Masini et al [108] went on to identify heterogeneity in response to acetylcholine with the virtual lack of sensitivity or by the full reaction to nanomolar concentrations of acetylcholine, observed in samples of serosal mast cells isolated from rats. The incubation with isolated rat mast cells with IgE induced a homogenous 'responder' population, with acetylcholine-induced histamine release proportional to the IgE concentration.…”

Section: Cholinergic Neuronsmentioning

confidence: 99%

The Mast Cell-Nerve Functional Unit: A Key Component of Physiologic and Pathophysiologic Responses

Forsythe¹,

Bienenstock²

2012

Chemical Immunology and Allergy

View full text Add to dashboard Cite

A key characteristic of mast cells appears to be an ability to span the division between nervous and immune system. Indeed, much of our understanding of the bi-directional relationship between the nervous and immune systems has come from the study of mast cell-nerve interaction. Although differences in species have been reported, morphologic as well as functional associations between mast cell and nerves are found in most tissues in many mammalian species, including humans. These interactions are involved in the regulation of physiologic homeostatic processes as well as in disease mechanisms. Here we discuss the influence of cholinergic and sensory neurons on mast cells as well as the importance of mast cell nerve interactions at specific tissue sites, including the brain.

show abstract

“…3A). Mast cells have AChRs, and degranulation of mast cells is regulated by these receptors111213. Destruction of the skin barrier on day seven may have involved the degranulation of mast cells via cholinergic signals.…”

Section: Discussionmentioning

confidence: 99%

Influence of Repeated Senna Laxative Use on Skin Barrier Function in Mice

et al. 2017

View full text Add to dashboard Cite

BackgroundSenna, one of the major stimulant laxatives, is widely used for treating constipation. Chronic senna use has been reported to be associated with colonic disorders such as melanosis coli and/or epithelial hyperplasia. However, there is no obvious information on the influence of chronic senna use on organs except for the intestine.ObjectiveTo clarify the influence of senna laxative use on skin barrier function by repeated senna administration.MethodsEight-week-old male hairless mice received senna (10 mg/kg/day) for 21 days. After administration, we evaluated transepidermal water loss (TEWL), and investigated the biomarkers in plasma and skin using protein analysis methods.ResultsFecal water content on day seven was significantly increased; however, on day 21, it was significantly decreased after repeated senna administration. In the senna-administered group, TEWL was significantly higher compared to the control on days seven and 21. Plasma acetylcholine concentration and NO2 −/NO3 − were increased on days seven and 21, respectively. In skin, tryptase-positive mast cells and inducible nitric oxide synthase (iNOS)-positive cells were increased on days seven and 21, respectively. The increase of TEWL on days seven and 21 was suppressed by the administration of atropine and N(G)-nitro-L-arginine methyl ester, respectively.ConclusionIt was suggested that diarrhea or constipation induced by repeated senna administration caused the impairment of skin barrier function. There is a possibility that this impaired skin barrier function occurred due to degranulation of mast cells via cholinergic signals or oxidative stress derived from iNOS.

show abstract

Mast Cell Heterogeneity in Response to Cholinergic Stimulation

Cited by 23 publications

References 3 publications

Methacholine challenge does not affect bronchoalveolar fluid cell number and many indices of cell function in asthma

Methacholine challenge does not affect bronchoalveolar fluid cell number and many indices of cell function in asthma

The Mast Cell-Nerve Functional Unit: A Key Component of Physiologic and Pathophysiologic Responses

Influence of Repeated Senna Laxative Use on Skin Barrier Function in Mice

Contact Info

Product

Resources

About