Mast cell numbers in incisional wounds in rat skin as a function of distance, time and treatment

Persinger, Michael A.; Lepage, Patricia; Simard, Jules; Parker, G. H.

doi:10.1111/j.1365-2133.1983.tb00060.x

Cited by 73 publications

(43 citation statements)

References 8 publications

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“…19 Because a strong mast cell degranulation response occurs in the skin following injury, a reduction or disappearance of mast cells is often described in wounds stained with granule-specific dyes. [20][21][22] In addition to releasing mediators through degranulation, recent studies have demonstrated that mast cells are also capable of releasing mediators without undergoing complete degranulation. 17 Mast cells can secrete individual granules or a subset of granules.…”

Section: Mast Cell Biologymentioning

confidence: 99%

“…Mast cell activation begins immediately after injury, which is evident based on reduced numbers of fully granulated mast cells and the presence of extracellular granules in the tissue. [20][21][22] Not surprisingly, given that they are resident inflammatory cells, mast cells play an important part in the inflammatory phase of healing. Several mediators stored in mast cell granules, like histamine and TNF-a, stimulate inflammation.…”

Section: Mast Cell Biologymentioning

confidence: 99%

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The Importance of Mast Cells in Dermal Scarring

Wilgus

Wulff

2014

Advances in Wound Care

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Section: Mast Cell Biologymentioning

confidence: 99%

Section: Mast Cell Biologymentioning

confidence: 99%

The Importance of Mast Cells in Dermal Scarring

Wilgus

Wulff

2014

Advances in Wound Care

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“…In a number of fibrosing processes, including wound healing, mast cells have been found to be strikingly increased in number, with an associated elevation of tissue histamine (12)(13)(14)(15)(16)(17)(18). In contrast, a temporary decrease of mast cells has been noted during the initiation of these diseases and during the first 24 h after wounding, using toluidine blue or avidin which stain the cytoplasmic granules of mast cells.…”

Section: Mast Cell Dynamics During Wound Healing and Fibrosismentioning

confidence: 99%

Mast cells and their mediators in cutaneous wound healing – active participants or innocent bystanders?

Artuc

Hermes

Steckelings

et al. 1999

Experimental Dermatology

216

143

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Mast cells are traditionally viewed as effector cells of immediateNeukölln, Berlin, Germany type hypersensitivity reactions. There is, however, a growing body of evidence that the cells might play an important role in the maintenance of tissue homeostasis and repair. We here present our own data and those from the literature elucidating the possible role of mast cells during wound healing. Studies on the fate of mast cells in scars of varying ages suggest that these cells degranulate during wounding, with a marked decrease of chymase-positive cells, although the total number of cells does not decrease, based on SCF-receptor staining. Mast cells contain a plethora of preformed mediators like heparin, histamine, tryptase, chymase, VEGF and TNF-a which, on release during the initial stages of wound healing, affect bleeding and subsequent coagulation and acute inflammation. Various additional vasoactive and chemotactic, rapidly generated mediators (C3a, C5a, LTB 4 , LTC 4 , PAF) will contribute to these processes, whereas mast cell-derived proinflammatory and growth promoting peptide mediators (VEGF, FGF-2, PDGF, TGF-b, NGF, IL-4, IL-8) contribute to neoangiogenesis, fibrinogenesis or re-epithelization during the repair process. The increasing number of tryptase-positive mast cells in older Key words: mast cells -keratinocytesfibroblasts -wound healing -angiogenesis scars suggest that these cells continue to be exposed to specific chemotactic, growth-and differentiation-promoting factors throughout the proProf. Dr B. M. Henz, Exp. Dermatology, cess of tissue remodelling. All these data indicate that mast cells contribCharité-Virchow Clinic,

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“…However, it was more abundant in the deeper dermis than in the superficial dermis in rats (11,23). In studies conducted on mast cells from the different areas of the skin, the highest number of mast cells was reported in the pinna (1,12,16).…”

Section: Discussionmentioning

confidence: 99%

Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques

KURTDEDE¹

2005

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:This study was conducted with the aim of examining the location, the morphology, and numerical distribution of mast cells in the different areas of the healthy adult dog skin. Skin samples taken from the cheek, pinna, thorax and thigh areas of 10 healthy dogs were used as the study material. The results show the existence of mast cell heterogeneity in dog skin with respect to staining with alcian blue / safranin O and formaldehyde fixation sensitivity. Mast cells characterized by four combination of these parameters were observed: Mast cells containing only alcian blue-positive granules which were formalin-sensitive, mast cells containing only alcian blue-positive granules which were formalin-resistant, mast cells containing only safranin O-positive granules which were formalin resistant and mast cells containing a mixture of alcian blue-positive and safranin O-positive granules which were formalin resistant. With toluidine blue staining method, it was founded that IFAA fixed tissues were contained more mast cells than those tissues fixed with the 10% formaldehyde in all four regions. The difference in average number of mast cells per mm² between the IFAA and the 10% formaldehyde fixed tissues was statistically significant (p<0.001). In the cheek area, whilst the difference between superficial dermis and deeper dermis in terms of the average mast cell count per mm² was not statistically significant (p>0.05), in the other three areas, statistically significant differences (p<0.001) were found. In the pinna, thorax and thigh areas, superficial dermis contained more mast cells than deeper dermis. In both fixations the highest number of mast cells was observed in the skin of the pinna (p<0.001).Key words: Distribution, dog skin, heterogeneity, mast cell. Köpek derisindeki mast hücreleri: dağılım,yoğunluk, heterojenite ve tespit tekniklerinin etkisiÖzet: Bu araştırma sağlıklı erişkin köpeklerde farklı deri bölgelerindeki mast hücrelerinin yerleşim yerlerini, morfolojilerini ve sayısal dağılımlarını incelemek amacıyla yapıldı. Çalışmada materyal olarak 10 adet sağlıklı köpeklerin yanak, kulak kepçesi, kaburga ve but bölgelerinden alınan deri örnekleri kullanıldı. Bulgularımız, alcian blue / safranin O ile boyanmasına ve formaldehit tespitine duyarlılığına göre köpek derisinde mast hücre heterojenitesini gösterdi. Mast hücreleri bu parametrelerin dört kombinasyonu ile tanımlandı: Formole duyarlı sadece alcian blue-pozitif granüller içeren mast hücreleri, formole dirençli sadece alcian blue-pozitif granüller içeren mast hücreleri, formole dirençli sadece safranin O-pozitif granüller içeren mast hücreleri ve formole dirençli hem safranin O-pozitif hem de alcian blue-pozitif mikst granüller içeren mast hücreleri. Toluidin blue ile boyanmış kesitlerde dört bölgede de IFAA tespitli dokuların % 10 formolle tespit edilenlere göre daha fazla mast hücresi içerdiği dikkati çekti. Bu iki tespit arasında mm²'de mast hücre sayı ortalamalarındaki farklılığın istatistiksel olarak önemli (p<0.001) olduğu saptandı. Yanak bölges...

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Mast cell numbers in incisional wounds in rat skin as a function of distance, time and treatment

Cited by 73 publications

References 8 publications

The Importance of Mast Cells in Dermal Scarring

The Importance of Mast Cells in Dermal Scarring

Mast cells and their mediators in cutaneous wound healing – active participants or innocent bystanders?

Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques

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