Mast cells and IgE-bearing cells in lungs of RAO-affected horses

Haegen, A. van der; Künzle, F.; Gerber, V.; Welle, Monika Maria; Robinson, N. E.; Marti, Eliane

doi:10.1016/j.vetimm.2005.06.005

Cited by 24 publications

(19 citation statements)

References 46 publications

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“…In addition, the airway inflammation may have partially resolved by 24 h as neutrophil infiltration into the pulmonary epithelial lining fluid occurs within 6 h of organic dust nebulization in RAO horses (29). Inhaled dust may induce the late-phase neutrophilic infiltration in RAO observed in the present study by enhancing the production of proinflammatory mediators from a range of cell types in addition to mast cells, such as alveolar macrophages, lymphocytes, and airway epithelial cells (6,7,24,41). Reduced numbers of macrophages and lymphocytes were evident in control horses immediately after challenge.…”

Section: Discussionmentioning

confidence: 57%

“…Enhanced production of IL-4 and IL-13 may elevate allergen-specific IgE generation from B cells during the early stages of RAO (20). IgE-mediated reactions do not appear to be important in the late-phase response in RAO (41).…”

mentioning

confidence: 97%

“…However, histaminase activity in the lung (22) may have resulted in the degradation of histamine released during an early-phase response by the time the 30-min sample was collected. Mast cells that contain chymase rather than tryptase appear to play a role in RAO, with the number of chymasepositive mast cells correlating with the severity of neutrophil infiltration (41). Mediators that are released from mast cells, but not activated neutrophils, have been implicated in the induction of bronchoconstriction in RAO (28).…”

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confidence: 99%

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Early onset airway obstruction in response to organic dust in the horse

Deaton

Deaton²,

Jose‐Cunilleras³

et al. 2007

Journal of Applied Physiology

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Deaton CM, Deaton L, Jose-Cunilleras E, Vincent TL, Baird AW, Dacre K, Marlin DJ. Early onset airway obstruction in response to organic dust in the horse. J Appl Physiol 102: [1071][1072][1073][1074][1075][1076][1077] 2007. First published December 7, 2006; doi:10.1152/japplphysiol.00264.2006.-Equine recurrent airway obstruction (RAO) has been used as a naturally occurring model of human asthma. However, it is unknown whether there is an early-phase response in RAO. The aim of this study was to determine whether exposure to organic dust induces immediate changes in lung function in RAO-affected horses, which could be mediated by airway mast cells. Six RAO-affected horses in remission and six control horses were challenged with hay-straw dust suspension by nebulization. Total respiratory resistance at 1 Hz, measured by forced oscillation, was increased from 0.62 Ϯ 0.09 cmH 2O ⅐ l Ϫ1 ⅐ s (mean Ϯ SE) to 1.23 Ϯ 0.20 cmH2O ⅐ l Ϫ1 ⅐ s 15 min after nebulization in control horses (P ϭ 0.023) but did not change significantly in the RAO group. Total respiratory reactance at 1 Hz (P ϭ 0.005) was significantly lower in the control horses (Ϫ0.77 Ϯ 0.07 cmH2O ⅐ l Ϫ1 ⅐ s) than in the RAO group (Ϫ0.49 Ϯ 0.04 cmH2O ⅐ l Ϫ1 ⅐ s) 15 min after nebulization. Bronchoalveolar lavage fluid (BALF) histamine concentration was significantly elevated 10 and 20 min postnebulization in control horses but not in RAO horses. Minimum reactance at 1 Hz in the early postnebulization period significantly correlated with both prechallenge BALF mast cell numbers (r ϭ Ϫ0.65, P ϭ 0.02) and peak BALF histamine concentration postnebulization (r ϭ Ϫ0.61, P ϭ 0.04). In conclusion, RAO horses, unlike human asthmatic patients, do not exhibit an early-phase response. However, healthy control horses do demonstrate a mild but significant early (Ͻ20 min) phase response to inhaled organic dust. This response may serve to decrease the subsequent dose of dust inhaled and as such provide a protective mechanism, which may be compromised in RAO horses. equine; lung function; allergen HUMAN ASTHMATIC PATIENTS develop an early-phase bronchoconstriction within minutes of exposure to inhaled allergens.

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Section: Discussionmentioning

confidence: 57%

mentioning

confidence: 97%

mentioning

confidence: 99%

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Early onset airway obstruction in response to organic dust in the horse

Deaton

Deaton²,

Jose‐Cunilleras³

et al. 2007

Journal of Applied Physiology

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“…Affected horses often present with exercise intolerance (Deegen 1981), coughing and dyspnoea (van der Haegen et al 2005). Affected horses show an increased reactivity to aerosolised particles such as fungal hyphae found in mouldy hay or straw.…”

Section: Introductionmentioning

confidence: 99%

Study on bronchoconstriction induced by histamine or serotonin in “Precision Cut Lung Slices” of the horse

Barton¹,

Wabnitz²,

Kietzmann³

et al. 2013

PHK

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SummaryThe aim of this study was to determine the effect of histamine and serotonin, as well as the influence of their inhibitors, on bronchoconstriction using equine precision-cut lung slices (PCLS), which were prepared from 5 horses affected by various degrees of small airway disease (SAD) and a sixth horse free of respiratory disease. Over all horses, histamine caused a concentration-dependent bronchoconstriction, with a median effective concentration of 1820 nM. The histamine induced bronchoconstriction could be inhibited by incubation with the H1-receptor antagonist cetirizine before the addition of histamine. However, incubation with the H2-receptor-antagonist ranitidine, the H2 and H3-receptor antagonist thioperamid and the H4-receptor antagonist JNJ7777120 had no significant effect. Serotonin induced a concentration-dependent bronchoconstriction in the horse without evidence of airway disease only. The serotonin-induced reaction in this case was inhibited when the PCLS was previously incubated in ketanserin. The possibility that respiratory disease could have a negative effect on the serotonin-receptor or the serotonin-induced bronchoconstriction pathway is discussed.Keywords: Bronchoconstriction / histamine / serotonin / PCLS / horse / pulmology / chronic airway disease Untersuchung der durch Histamin oder Serotonin induzierten Bronchokonstriktion in "Precision Cut Lung Slices" des Pferdes Das Ziel der Studie lag in der Untersuchung des Effektes von Histamin und Serotonin sowie des Einflusses ihrer Inhibitoren auf die

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“…Similar to other allergic diseases, in RAO-affected horses, reaginic antibodies bind through their Fc fragment to high affinity receptors (FcεRI) present in the membrane of mast cells and basophils. The sensitizing antibodies bound to the mast cell membrane are subsequently cross-linked by allergen exposure, which results in mast cell degranulation with the production of histamine, serotonin, leukotrienes, tromboxane, and 15 HETE that in turn induces airway inflammation (van der Haegen et al 2005). Consequently bronchospasm, mucus secretion, bronchiolar wall thickening, and obstructions in the respiratory tract are common (Derksen et al 1985;Gerber et al 2004).…”

Section: Introductionmentioning

confidence: 99%

In vitro bioassay to detect reaginic antibodies from the serum of horses affected with Recurrent Airway Obstruction

et al. 2009

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In horses, Recurrent Airway Obstruction (RAO) is an allergic disease that involves IgE mediated Type I Hypersensitivity responses. The development of this type of allergy involves a series of events that begins with reaginic antibodies, mainly IgE and some IgG subclasses. These reaginic antibodies bind with high affinity, via the Fc portion, to FcepsilonRI receptors on the membrane of mast cells and basophils. Once bound, environmental allergens cross-link the antibodies, which results in mast cell degranulation leading to the production of histamine and other chemical mediators that act together to induce airway inflammation. RAO-affected horses present with coughing, respiratory distress, airway obstruction and poor performance. The aspect of the RAO has been extensively studied, yet the precise sequence of events is still not well understood. Therefore, this study proposes a bioassay for reaginic antibody detection from horse serum of RAO-affected individuals, in order to determine the etiology of disease, which mediate immediate type reactions. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from affected or unaffected horses and one of the RAO antigens (Aspergillus fumigatus). The results presented here demonstrate that 30% of RAO-affected horses react positively in this in vitro bioassay, whereas unaffected horses do not. This bioassay may facilitate further research on RAO and other allergic diseases in horses.

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Mast cells and IgE-bearing cells in lungs of RAO-affected horses

Cited by 24 publications

References 46 publications

Early onset airway obstruction in response to organic dust in the horse

Early onset airway obstruction in response to organic dust in the horse

Study on bronchoconstriction induced by histamine or serotonin in “Precision Cut Lung Slices” of the horse

In vitro bioassay to detect reaginic antibodies from the serum of horses affected with Recurrent Airway Obstruction

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