Mastitis detection: current trends and future perspectives

Viguier, Caroline; Arora, Sushrut; Gilmartin, Niamh; Welbeck, Katherine; O’Kennedy, Richard

doi:10.1016/j.tibtech.2009.05.004

Cited by 419 publications

(355 citation statements)

References 64 publications

Supporting

Mentioning

346

Contrasting

Unclassified

Order By: Relevance

“…If virus-infected cells are efficiently destroyed by H5N1, blood is released into the intestinal lumen, thereby increasing the pH. A similar phenomenon is observed in bovine mastitis, in which bacterial infection damages the blood-milk barrier resulting in increased pH values in milk (50). We previously reported that H5N1 viruses induce apoptosis in duck embryonic fibroblasts and mammalian airway epithelial cells (10,51), supporting the hypothesis that luminal pH changes occur in the intestine of H5N1-infected birds.…”

Section: Discussionsupporting

confidence: 59%

Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH

Daidoji

Watanabe

Ibrahim

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background:The mechanism of H5N1 pathogenesis in humans remains unclear. Results: SAEC-T clones were poorly susceptible to previously circulating avian influenza viruses but were completely susceptible to H5N1. Conclusion: Infectivity depends on a delicate balance between acid stability of viral hemagglutinin and endosomal pH in infected cells. Significance: These findings could explain why H5N1 is directly transmitted to humans from birds, resulting in serious illness.

show abstract

Section: Discussionsupporting

confidence: 59%

Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH

Daidoji

Watanabe

Ibrahim

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…A number of diagnostic options exist for the identification of mastitis but they have differences with respect to accuracy and cost (Emanuelson et al, 1987;Pyorala, 2003;Viguier et al, 2009). A difficulty in the estimation of diagnostic accuracy is the lack of a gold standard for the classification of cattle as having mastitis (Dohoo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Sensitivity and specificity of a hand-held milk electrical conductivity meter compared to the California mastitis test for mastitis in dairy cattle

Fosgate

Petzer

Karzis

2013

The Veterinary Journal

View full text Add to dashboard Cite

Screening tests for mastitis can play an important role in proactive mastitis control programs to reduce the economic impact of this disease. The primary objective of this study was to compare the accuracy of milk electrical resistance (MER) to the California milk cell test (CMCT) in commercial dairy cattle of South Africa using Bayesian methods without a perfect reference test. A total of 1,848 quarter milk specimens were collected from 173 cows sampled during 6 sequential farm visits and 25.8% yielded pathogenic bacterial isolates.Most frequently isolated bacterial species were coagulase negative Staphylococci (n = 346), Streptococcus agalactiae (n = 54), and Staphylococcus aureus (n = 42). The overall cowlevel prevalence of mastitis was 54% based on the Bayesian latent class analysis and the prevalence varied by quarter. The CMCT was more accurate than MER for classification of cows as having somatic cell counts >200,000/ml and isolation of a bacterial pathogen.Bayesian latent class analysis also suggested an overall benefit of CMCT over MER but the difference was not highly statistically probable. The Bayesian model estimated the sensitivity and specificity of MER at a cut-point of >25 mΩ/cm to be 89.9% and 86.8% respectively.The CMCT had a sensitivity and specificity of 94.5% and 77.7% respectively when evaluated at the slight positive cut-point. Milk electrical resistance was useful for identifying milk specimens harboring pathogens but was not able to differentiate among evaluated bacterial isolates. Screening tests can be used to improve udder health as part of a proactive management plan.

show abstract

“…Additionally, mastitis may be caused by slow-growing bacteria, such as Mycoplasma spp., which require special growth medium, thus delaying diagnosis. To overcome these problems, PCR-based methods have been developed; they are frequently restricted to a limited number of pathogens, however (Viguier et al, 2009;Ajitkumar et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PCR electrospray-ionization mass spectrometry for rapid molecular diagnosis of bovine mastitis

Perreten

Endimiani

Thomann

et al. 2013

Journal of Dairy Science

View full text Add to dashboard Cite

Bovine mastitis, an inflammatory disease of the mammary gland, is one of the most costly diseases affecting the dairy industry. The treatment and prevention of this disease is linked heavily to the use of antibiotics in agriculture and early detection of the primary pathogen is essential to control the disease. Milk samples (n = 67) from cows suffering from mastitis were analyzed for the presence of pathogens using PCR electrosprayionization mass spectrometry (PCR/ESI-MS) and were compared with standard culture diagnostic methods. Concurrent identification of the primary mastitis pathogens was obtained for 64% of the tested milk samples, whereas divergent results were obtained for 27% of the samples. The PCR/ESI-MS failed to identify some of the primary pathogens in 18% of the samples, but identified other pathogens as well as microorganisms in samples that were negative by culture. The PCR/ ESI-MS identified bacteria to the species level as well as yeasts and molds in samples that contained a mixed bacterial culture (9%). The sensitivity of the PCR/ESI-MS for the most common pathogens ranged from 57.1 to 100% and the specificity ranged from 69.8 to 100% using culture as gold standard. The PCR/ESI-MS also revealed the presence of the methicillin-resistant gene mecA in 16.2% of the milk samples, which correlated with the simultaneous detection of staphylococci including Staphylococcus aureus. We demonstrated that PCR/ESI-MS, a more rapid diagnostic platform compared with bacterial culture, has the significant potential to serve as an important screening method in the diagnosis of bovine clinical mastitis and has the capacity to be used in infection control programs for both subclinical and clinical disease.

show abstract

Mastitis detection: current trends and future perspectives

Cited by 419 publications

References 64 publications

Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH

Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH

Sensitivity and specificity of a hand-held milk electrical conductivity meter compared to the California mastitis test for mastitis in dairy cattle

Evaluation of PCR electrospray-ionization mass spectrometry for rapid molecular diagnosis of bovine mastitis

Contact Info

Product

Resources

About