Mastoparans: A Group of Multifunctional α-Helical Peptides With Promising Therapeutic Properties

Santana, Carlos José Correia de; Júnior, Osmindo Rodrigues Pires; Fontes, Wagner; Palma, Mário Sérgio; Castro, Mariana S.

doi:10.3389/fmolb.2022.824989

Cited by 28 publications

(26 citation statements)

References 147 publications

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“…Hymenoptera venoms consist of a complex mixture of chemically or pharmacologically bioactive components including phospholipases, hyaluronidases and mastoparans [ 69 ]. Mastoparans, a group of alpha-helical peptides, appear to be the most promising for pharmacological activities [ 37 ] since they are able to penetrate into cells and bind DNA [ 70 ]. Melectin, an antimicrobial peptide from the venom of the cleptoparasitic bee Melecta albifrons , does not exhibit sequence homology with other wasp venom peptides.…”

Section: Results Of Literature Searchmentioning

confidence: 99%

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Sjakste

Gajski

2023

Toxins

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Envenomation by animal venoms remains a serious medical and social problem, especially in tropical countries. On the other hand, animal venoms are widely used as a source of biologically active compounds for the development of novel drugs. Numerous derivatives of animal venoms are already used in clinical practice. When analysing the mechanisms of action of animal venoms, attention is usually focused on the main target of the venom’s enzymes and peptides such as neurotoxic, cytotoxic or haemorrhagic effects. In the present review, we would like to draw attention to the “hidden” effects of animal venoms and their derivatives in regard to DNA damage and/or protection against DNA damage. Alkaloids and terpenoids isolated from sponges such as avarol, ingenamine G or variolin B manifest the capability to bind DNA in vitro and produce DNA breaks. Trabectidin, isolated from a sea squirt, also binds and damages DNA. A similar action is possible for peptides isolated from bee and wasp venoms such as mastoparan, melectin and melittin. However, DNA lesions produced by the crude venoms of jellyfish, scorpions, spiders and snakes arise as a consequence of cell membrane damage and the subsequent oxidative stress, whereas certain animal venoms or their components produce a genoprotective effect. Current research data point to the possibility of using animal venoms and their components in the development of various potential therapeutic agents; however, before their possible clinical use the route of injection, molecular target, mechanism of action, exact dosage, possible side effects and other fundamental parameters should be further investigated.

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Section: Results Of Literature Searchmentioning

confidence: 99%

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Sjakste

Gajski

2023

Toxins

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“…Nọc độc của những loài côn trùng và lưỡng cư là một trong những nguồn quan trọng cung cấp các AMP tiềm năng [1][2][3]. Trong số đó, ong bắp cày được nghiên cứu nhiều nhất [4][5][6]. Tác dụng kháng khuẩn tốt đối với nhiều vi sinh vật gây bệnh bao gồm cả một số chủng kháng thuốc trên lâm sàng và khả năng gây tán huyết thấp đã làm cho các hợp chất này trở thành những ứng cử viên tiềm năng để phát triển thành thuốc chống nhiễm trùng [3].…”

Section: ________unclassified

“…Khoảng 40 chuỗi peptid từ nọc ong bắp cày sống theo đàn và 6 chuỗi từ những loài đơn độc đã được mô tả. Kết quả nghiên cứu đã chứng tỏ các peptid thuộc họ này có nhiều tác dụng sinh học như kích hoạt protein G, kích hoạt phospholipase A2, C và D, giải phóng serotonin và insulin, kháng khuẩn, tán huyết, chống ung thư, thúc đẩy sự gia tăng Ca 2+ nội bào nhằm điều chỉnh quá trình giao tiếp giữa các tế bào [6].…”

Section: ________unclassified

“…Đây là một chuỗi peptid ngắn gồm 14 acid amin với trình tự Leu-Asn-Leu-Lys-Ala-Leu-Leu-Ala-Val-Ala-Lys-Lys-Ile-Leu-NH2 hay H-LNLKALLAVAKKIL-NH2. Nhóm amino ở đầu N tồn tại dưới dạng tự do và nhóm carboxyl ở đầu C được amid hoá [6,10]. Phân tử không chứa các acid amin đặc biệt như Cystein và Prolin.…”

Section: Cấu Trúc Bậc Mộtunclassified

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Potentialities of Antimicrobial Peptide Mastoparan C Isolated the European Hornet Vespa Crabro in Drug Discovery

Hai¹,

Anh²,

Huyen³

et al. 2023

MPS

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Exploring the potential of natural bioactive peptides is becoming more and more important for new drug discovery. First isolated from the European wasp Vespa crabro, the antimicrobial peptide Mastoparan C was found in higher content than many other molecules in the Mastoparan family. In addition to the mast cell degranulation that is similar to some components in insect venom, this compound possesses remarkable biological activities such as broad-spectrum antibacterial and antifungal, inhibiting the proliferation of many human cancer cell lines. However, unfortunately, Mastoparan C has the notable side effect of causing mammalian hemolysis. By investigating its structure-activity relationships, this review pointed out some suggestions to overcome the disadvantages of this potential peptide and thereby support further in-depth study in the drug discovery field. Keywords: Mastoparan C, antimicrobial peptide, biological activity, structure-activity relationships.

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“…In contrast, Mastoparan-L was only active against Gram-positive bacteria and some strains of Brucella abortus, while having limited effect on probiotics. 35 Furthermore, in the previous report, we compared the antimicrobial properties of nano-titanium dioxide (TiO 2 -NP), and nano-zinc oxide (ZnO NP), with two synthetic peptides (MP-B and Indolicidin) against MDR strains of P. aeruginosa, and hypervirulent strains of K. pneumoniae. 36 Nevertheless, it was found that combining antibiotics with MP-B at the sub-MIC level circumvented the resistance phenomenon and significantly reduced antibiotic dose.…”

Section: Introductionmentioning

confidence: 99%

Amphiphilic peptide Mastoparan‐B induces conformational changes within the AdeB efflux pump, down‐regulates adeB gene expression, and restores antibiotic susceptibility in an MDR strain of Acinetobacter baumannii

et al. 2023

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Mastoparan B (MP‐B) is an amphiphilic peptide with a potent antimicrobial activity against most Gram‐negative bacteria. However, there is little information available on the inhibition of the Acinetobacter baumannii resistance‐nodulation‐cell‐division (RND) efflux pump using this antimicrobial peptide. Here, we carried out a series of in‐silico experiments to find the mechanisms underlying the anti‐efflux activity of MP‐B using a multi‐drug resistant (MDR) strain of A. baumannii (AB). According to our findings, MP‐B demonstrated a potent antibacterial activity against an MDR‐AB (minimum inhibitory concentration [MIC] = 1 μg/mL) followed by a 20‐fold reduction in the adeB gene expression in the presence of sub‐MIC of this peptide. Using Groningen Machine for Chemicals Simulation (GROMACS) via PyMOL Graphical User Interface (GUI), (we observed that, the AdeB transporter had conserved helix‐turn‐helix regions and a tight pore rich in Phe and Ala residues. To understand how inhibition of the AdeB is achieved, we generated 20 apo‐MP‐B poses using the InterPep and SiteMap tools. The high‐quality model was created by homology modeling and used for docking via AutoDock/Vina to identify the MP‐B binding sites. We established that the most apo‐MP‐B formed H‐bonds to the backbone of five amino acids in the Helix‐5. As a result, the dihedral angles of the involved amino acids shift by 9.0–9.6 Ǻ, causing a change in the conformation of the AdeB protein. This led to helix conformation stereoisomerization and block the AdeB activity. MP‐B presumably has dual mechanisms. (1) It blocks the AdeB transporter by changing its conformation. (2) MP‐B influences the adeB gene expression by binding to G‐protein which laterally controls efflux regulators like MarA, RamA, SoxS, and Rob proteins.

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Mastoparans: A Group of Multifunctional α-Helical Peptides With Promising Therapeutic Properties

Cited by 28 publications

References 147 publications

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Potentialities of Antimicrobial Peptide Mastoparan C Isolated the European Hornet Vespa Crabro in Drug Discovery

Amphiphilic peptide Mastoparan‐B induces conformational changes within the AdeB efflux pump, down‐regulates adeB gene expression, and restores antibiotic susceptibility in an MDR strain of Acinetobacter baumannii

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