Matched filtering Generalized Phase Contrast using binary phase for dynamic spot- and line patterns in biophotonics and structured lighting

Bañas, Andrew Rafael; Aabo, Thomas; Palima, Darwin; Glückstad, Jesper

doi:10.1364/oe.21.001849

Cited by 6 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even in a low NA counter-propagating optical trap setup, the smaller beam size and 3D addressing o ered by holography can enable more e cient light coupling into 3D-maneuvered waveguide microtools [73]. These limitations in peak intensities and lack of 3D addressing have thus motivated the development of hybrid techniques such as matched ltering GPC [74] and Holo-GPC [75] that will be discussed later.…”

Section: Laser Shaping Applications That Are Not Optimal For Gpcmentioning

confidence: 99%

Light Shaping with Holography, GPC and Holo-GPC

Bañas¹,

Glückstad²

2017

Optical Data Processing and Storage

Self Cite

View full text Add to dashboard Cite

Light shaping techniques based on phase-only modulation o er multiple advantages over amplitude modulation. This review examines and compares the merits of two phase modulation techniques; phase-only computer generated holography and Generalized Phase Contrast (GPC). Both techniques are brie y presented while recent developments in GPC will also be covered. Furthermore, novel hybrid schemes that inherit merits from both holography and GPC are also covered. In particular, our most recent technique coined "Holo-GPC" will be discussed in addition to earlier hybrid techniques. We will discuss how Holo-GPC utilizes the simplicity of GPC in forming well-de ned speckle-free shapes and the versatility of holography in distributing these shaped beams over an extended 3D volume. To conclude, we cite applications where the combined strengths of the two photon-e cient phase-only light shaping techniques open new possibilities.

show abstract

Section: Laser Shaping Applications That Are Not Optimal For Gpcmentioning

confidence: 99%

Light Shaping with Holography, GPC and Holo-GPC

Bañas¹,

Glückstad²

2017

Optical Data Processing and Storage

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to Generalized Phase Contrast (GPC) [8] which have been applied in studying neurons and yeast cells [6,9], we have also been studying its variant, matched filtering GPC (mGPC) ( Fig. 1.1) due to its robustness to device imperfections that allow it to operate even in low cost consumer projectors [10,11]. Our studies and experiments with mGPC are presented in Chapter 4 of this thesis.…”

Section: Active Microscopy Through Beam Shapingmentioning

confidence: 99%

“…For optional sample illumination, an LED provides light which enters the system via the dichroic mirror. (Figure adapted from [11]).…”

Section: Generation Of High Intensity High Contrast Mgpc Spikesmentioning

confidence: 99%

See 1 more Smart Citation

A Next Generation BioPhotonics Workstation

Glückstad

Palima

Tauro

et al. 2011

Optics in the Life Sciences

View full text Add to dashboard Cite

, whose contributions remain relevant to our present work. The BRC group that hosted my external stay and fabricated the microtools that we trap, Lόránd Kelemen, Pál Ormos, Gaszton Vizsnyiczai, Badri Aekbote, András Búzás. Collaborators at Aarhus University and Copenhagen University as well as the people at the business development side for helping me appreciate the things I build in the laboratory. Rune Christiansen and the rest of DTU Danchip for fabricating our matched filters. And the Danish Research Council (FTP) for funding. I would also like to express my thanks to my examiners, Tomáš Čižmár and Simon Hanna whose comments have helped improved this thesis. Kirstine Berg-Sørensen, also for the helpful comments and for facilitating practical matters regarding the thesis. For the less technical aspects that nonetheless contribute to the grand scheme of things, as well as my mental health, I also thank my family for understanding my extended absence, my officemates and neighbours for standing in as co-PhDs, my very kind landlady, Birgit, for not minding all those scattered computers, cables and musical instruments, and the very nice or strange people I meet everyday at work.

show abstract

“…Shaped light, for example, has allowed higher yields in 2-photon photopolymerization (2PP) through parallelism [5] while maintaining a good fidelity by adaptively compensating for imperfections in setups, such as aberrations or improving the focusing properties of the writing light [6].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and optical trapping of handling structures for re-configurable microsphere magnifiers

Bañas

Vizsnyiczai

Búzás

et al. 2013

Complex Light and Optical Forces VII

Self Cite

View full text Add to dashboard Cite

We demonstrate the use of microfabricated supporting structures for maneuvering and supporting polystyrene microspheres for use as magnifying lenses in imaging applications. The supporting structure isolates the trapping light from the magnifier, hence avoiding direct radiation to the sample being observed which could be damaging, especially for biological specimens. Using an optical trapping setup, we demonstrate the actuation of a microsphere not held by optical traps, and show the possibility of imaging through such microspheres.

show abstract

Matched filtering Generalized Phase Contrast using binary phase for dynamic spot- and line patterns in biophotonics and structured lighting

Cited by 6 publications

References 16 publications

Light Shaping with Holography, GPC and Holo-GPC

Light Shaping with Holography, GPC and Holo-GPC

A Next Generation BioPhotonics Workstation

Fabrication and optical trapping of handling structures for re-configurable microsphere magnifiers

Contact Info

Product

Resources

About