Materials and Methods for Noise Reduction in Cannery Environments

Waggoner, S. A.; Shackelford, James F.; Robbins, F.F.; Burkhardt, Thomas; O'Brien, M.

doi:10.13031/2013.35431

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To measure the rate of volume changes elicited by energydependent extrusion of KC1 from the cells, the light scattering method in [1] was used. The crossmembranal ApH and A,/, were assessed with the aid of the fluorescent dyes 9-AA and Dis-C3-(5), respectively [8,9].…”

Section: Methodsmentioning

confidence: 99%

Valinomycin as a tool for the reversal of the effect of protonophoric uncouplers

1982

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Valinomycin as a tool for the reversal of the effect of protonophoric uncouplers

1982

View full text Add to dashboard Cite

“…Membrane potential in intact Indo-l-loaded platelets was estimated by using the fluorescent dye diS-Cr(5) based on the method described Published by Elsevier Science Publishers B. V. (Biomedical Division) in [22,23]. For calibration the cells were treated with valinomycin (1 #M), the extracellular K ÷ concentration was raised gradually and the changes of the fluorescence were followed (620 nm excitation and 670 nm emission wavelengths).…”

Section: For the [Cag÷]i Measurements Platelets Were Loaded Inmentioning

confidence: 99%

Membrane depolarization inhibits thrombin‐induced calcium influx and aggregation in human platelets

et al. 1990

View full text Add to dashboard Cite

The relationship between thrombin-evoked changes in intracellular calcium concentration ([Ca2+]i) and aggregation was examined in Indo-l-loaded human platelets. The stimulus-induced intracellular calcium release and external calcium influx, as well as platelet aggregation, were studied in the same cell preparation. A close correlation between the sustained high [Ca2+]i level, depending on calcium entry, and the aggregation response was found. Gramicidin, at a concentration high enough to induce membrane depolarization, strongly inhibited the calcium influx and aggregation, but did not influence the thrombin-induced intracellular calcium release. We conclude that calcium influx through depolarization-inhibited calcium channels is a prerequisite of thrombin-induced platelet aggregation.

show abstract

“…Artificially imposed A y, interior negative, was recorded using the fluorescence quenching of diS-C3-(5) [27]. Membrane vesicles (0.1 mg protein) prepared in 50 mM potassium phosphate (pH 7.0) were diluted 100-fold into 50 mM sodium phosphate (pH 7.0) supplemented with 10 mM MgS04 and 2 pM diS-C3- (5).…”

Section: Measurement Of' the Proton Motive Forcementioning

confidence: 99%

Calcium transport in membrane vesicles of Streptococcus cremoris

Driessen¹,

Konings²

1986

European Journal of Biochemistry

View full text Add to dashboard Cite

Rightside‐out membrane vesicles of Streptococcus cremoris were fused with proteoliposomes containing the light‐driven proton pump bacteriorhodopsin by a low‐pH fusion procedure reported earlier [Driessen, A. J. M., Hellingwerf, K. J. & Konings, W. N. (1985) Biochim. Biophys. Acta 808, 1–12]. In these fused membranes a proton motive force, interior positive and acid, can be generated in the light and this proton motive force can drive the uptake of Ca2+. Collapsing ΔΨ with a concomitant increase in ΔpH stimulates Ca2+ uptake while dissipation of the ΔpH results in a reduced rate of Ca2+ uptake. Also an artificially generated ΔpH, interior acid, can drive Ca2+ uptake in S. cremoris membrane vesicles. Ca2+ uptake depends strongly on the presence of external phosphate while Ca2+‐efflux‐induced proton flux is independent of the presence of external phosphate. Ca2+ accumulation is abolished by the divalent cation ionophore A23187. Calcium extrusion from intact cells is accelerated by lactose. Collapse of the proton motive force by the uncoupler carbonylcyanide p‐trifluoromethoxyphenylhydrazone or inhibition of the membrane‐bound ATPase by N,N′‐dicyclohexylcarbodiimide strongly inhibits Ca2+ release. Further studies on Ca2+ efflux at different external pH values in the presence of either valinomycin or nigericin suggested that Ca2+ exit from intact cells is an electrogenic process. It is concluded that Ca2+ efflux in S. cremoris is mediated by a secondary transport system catalyzing exchange of calcium ions and protons.

show abstract

Materials and Methods for Noise Reduction in Cannery Environments

Cited by 3 publications

References 0 publications

Valinomycin as a tool for the reversal of the effect of protonophoric uncouplers

Valinomycin as a tool for the reversal of the effect of protonophoric uncouplers

Membrane depolarization inhibits thrombin‐induced calcium influx and aggregation in human platelets

Calcium transport in membrane vesicles of Streptococcus cremoris

Contact Info

Product

Resources

About