Materials and methods for the microfabrication of microfluidic biomedical devices

Wu, Wen-I; Rezai, Pouya; Hsu, Hsiao-Ting; Selvaganapathy, P.R.

doi:10.1533/9780857097040.1.3

Cited by 17 publications

(14 citation statements)

References 247 publications

(252 reference statements)

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“…Wet chemical etching is isotropic and produces rounded side wall microchannels. The depth of the these channels is controlled by the etch rate and etch duration whereas the width of the microchannel can be estimated by the mask opening plus twice the channel depth [49]. Therefore, high aspect ratio features are hard to build using these methods.…”

Section: Siliconmentioning

confidence: 99%

Recent developments and trends in miniaturized gas preconcentrators for portable gas chromatography systems: A review

Lara-Ibeas¹,

Cuevas²,

Calvé³

2021

Sensors and Actuators B: Chemical

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Section: Siliconmentioning

confidence: 99%

Recent developments and trends in miniaturized gas preconcentrators for portable gas chromatography systems: A review

Lara-Ibeas¹,

Cuevas²,

Calvé³

2021

Sensors and Actuators B: Chemical

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“…The device is made of superimposed polymethyl methacrylate (PMMA) sheets. This rigid thermoplastic is biocompatible, cost-effective, suitable for mass fabrication, and has excellent optical performance of light transmission [19,20]. A panel of different thicknesses of PMMA sheets is available.…”

Section: Materials For Device Fabricationmentioning

confidence: 99%

Blood–Brain Barrier Dynamic Device with Uniform Shear Stress Distribution for Microscopy and Permeability Measurements

et al. 2021

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Neurology has always been one of the therapeutic areas with higher attrition rates. One of the main difficulties is the presence of the blood–brain barrier (BBB) that restricts access to the brain for major drugs. This low success rate has led to an increasing demand for in vitro tools. The shear stress, which positively affects endothelial cell differentiation by mimicking blood flow, is required for a more physiological in vitro BBB model. We created an innovative device specifically designed for cell culture under shear stress to investigate drug permeability. Our dynamic device encompasses two compartments communicating together via a semi-permeable membrane, on which human cerebral microvascular endothelial (hCMEC/D3) cells were seeded. The fluidic controlled environment ensures a laminar and homogenous flow to culture cells for at least seven days. Cell differentiation was characterized by immunodetection of inter-endothelial junctions directly in the device by confocal microscopy. Finally, we performed permeability assay with lucifer yellow in both static and dynamic conditions in parallel. Our dynamic device is suited to the evaluation of barrier function and the study of drug transport across the BBB, but it could also be used with other human cell types to reproduce intestinal or kidney barriers.

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“…We note that the thickness of the walls between the meandering channels is omitted in the theoretical analysis. In practice, using state-of-the-art nanofabrication, wall thicknesses down to 300 nm are easily feasible due to well-established vertical etch processes, 26 thereby not significantly impacting the conclusions from our calculations. To investigate the analyte transport to the binding sites, we have calculated the mass transfer coefficient, k m , and compared it to values of k on Γ 0 typical for protein interactions ( Figure 2 e).…”

Section: Infinite Sample Volumementioning

confidence: 99%

Nanoplasmonic–Nanofluidic Single-Molecule Biosensors for Ultrasmall Sample Volumes

et al. 2020

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Detection of small amounts of biological compounds is of ever-increasing importance but also remains an experimental challenge. In this context, plasmonic nanoparticles have emerged as strong contenders enabling label-free optical sensing with single-molecule resolution. However, the performance of a plasmonic single-molecule biosensor is not only dependent on its ability to detect a molecule but equally importantly on its efficiency to transport it to the binding site. Here, we present a theoretical study of the impact of downscaling fluidic structures decorated with plasmonic nanoparticles from conventional microfluidics to nanofluidics. We find that for ultrasmall picolitre sample volumes, nanofluidics enables unprecedented binding characteristics inaccessible with conventional microfluidic devices, and that both detection times and number of detected binding events can be improved by several orders of magnitude. Therefore, we propose nanoplasmonic–nanofluidic biosensing platforms as an efficient tool that paves the way for label-free single-molecule detection from ultrasmall volumes, such as single cells.

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Materials and methods for the microfabrication of microfluidic biomedical devices

Cited by 17 publications

References 247 publications

Recent developments and trends in miniaturized gas preconcentrators for portable gas chromatography systems: A review

Recent developments and trends in miniaturized gas preconcentrators for portable gas chromatography systems: A review

Blood–Brain Barrier Dynamic Device with Uniform Shear Stress Distribution for Microscopy and Permeability Measurements

Nanoplasmonic–Nanofluidic Single-Molecule Biosensors for Ultrasmall Sample Volumes

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